Inhibitory effect of brassinin on TNF‑α‑induced vascular inflammation in human umbilical vein endothelial cells

Han,Byung  Hyuk; Yoon,Jung  Joo; Choi,Eun  Sik; Jeong,Da  Hye; Lee,Yun  Jung; Kang,Dae  Gill; Lee,Ho  Sub

doi:10.3892/mmr.2017.7406

Inhibitory effect of brassinin on TNF‑α‑induced vascular inflammation in human umbilical vein endothelial cells

Authors:
- Byung Hyuk Han
- Jung Joo Yoon
- Eun Sik Choi
- Da Hye Jeong
- Yun Jung Lee
- Dae Gill Kang
- Ho Sub Lee
View Affiliations

Affiliations: College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea
Published online on: August 31, 2017 https://doi.org/10.3892/mmr.2017.7406
Pages: 6890-6895

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Brassinin, a phytoalexin firstly identified as a constituent of Chinese cabbage, has been demonstrated to exhibit antiproliferative effects on various cancer cell lines, by reducing reactive oxygen species (ROS) production via regulation of the antioxidant pathway. The present study aimed to explore the protective effects of brassinin in TNF‑α‑induced vascular inflammation in human umbilical vein endothelial cells (HUVECs). Pretreatment with brassinin significantly inhibited adhesion of U937 cells to TNF‑α‑induced HUVECs in a dose‑dependent manner. Brassinin treatment decreased the expression levels of cell adhesion molecules, including intracellular adhesion molecule‑1 (ICAM‑1), vascular cell adhesion molecule‑1 (VCAM‑1), and endothelial‑selectin (E‑selectin) following stimulation with TNF‑α in HUVECs. In addition, pretreatment with brassinin decreased the protein expression levels of nuclear factor (NF)‑κB p65 in the nucleus, suggesting that brassinin inhibited NF‑κB p65 nuclear translocation. Brassinin treatment also markedly decreased the mRNA expression levels of interleukin‑8 in a dose‑dependent manner. Finally, brassinin pretreatment significantly decreased TNF‑α‑induced intracellular reactive oxygen species (ROS) production in HUVECs compared with control. The present results therefore suggest that brassinin may serve as a potential therapeutic agent for atherosclerosis.

View Figures

View References

1	Lee YJ, Moon MK, Hwang SM, Yoon JJ, Lee SM, Seo KS, Kim JS, Kang DG and Lee HS: Anti-Inflammatory effect of Buddleja officinalis on vascular inflammation in human umbilical vein endothelial cells. Am J Chin Med. 38:585–598. 2010. View Article : Google Scholar : PubMed/NCBI
2	Liu X, Pan L, Wang X, Gong Q and Zhu YZ: Leonurine protects against tumor necrosis factor-α-mediated inflammation in human umbilical vein endothelial cells. Atherosclerosis. 222:34–42. 2012. View Article : Google Scholar : PubMed/NCBI
3	Ross EA, Douglas MR, Wong SH, Ross EJ, Curnow SJ, Nash GB, Rainger E, Scheel-Toellner D, Lord JM, Salmon M and Buckley CD: Interaction between integrin alpha9beta1 and vascular cell adhesion molecule-1 (VCAM-1) inhibits neutrophil apoptosis. Blood. 107:1178–1183. 2006. View Article : Google Scholar : PubMed/NCBI
4	Sana TR, Janatpour MJ, Sathe M, McEvoy LM and McClanahan TK: Microarray analysis of primary endothelial cells challenged with different inflammatory and immune cytokines. Cytokines. 29:256–269. 2005.
5	Gerszten RE, Garcia-Zepeda EA, Lim YC, Yoshida M, Ding HA, Gimbrone MA Jr, Luster AD, Luscinskas FW and Rosenzweig A: MCP-1 and IL-8 trigger firm adhesion of monocytes to vascular endothelium under flow conditions. Nature. 398:718–723. 1999. View Article : Google Scholar : PubMed/NCBI
6	Tak PP, Gerlag DM, Aupperle KR, van de Geest DA, Overbeek M, Bennett BL, Boyle DL, Manning AM and Firestein GS: Inhibitor of nuclear factor kappaB kinase beta is a key regulator of synovial inflammation. Arthritis Rheum. 44:1897–1907. 2001. View Article : Google Scholar : PubMed/NCBI
7	Chen JW, Chen YH, Lin FY, Chen YL and Lin SJ: Ginkgo biloba extract inhibits tumor necrosis factor-alpha-induced reactive oxygen species generation, transcription factor activation, and cell adhesion molecule expression in human aortic endothelal cells. Arterioscler Thromb Vasc Biol. 23:1559–1566. 2003. View Article : Google Scholar : PubMed/NCBI
8	Iademarco MF, Mcquillan JJ, Rosen GD and Dean DC: Characterization of the promoter for vascular cell adhesion molecule-1 (VCAM-1). J Biol Chem. 267:16323–16329. 1992.PubMed/NCBI
9	Gloire G, Legrand-Poels S and Piette J: NF-kappaB activation by reactive oxygen species: Fifteen years later. Biochem Pharmacol. 72:1493–1505. 2006. View Article : Google Scholar : PubMed/NCBI
10	Lee SM, Lee YJ, Kim YC, Kim JS, Kang DG and Lee HS: Vascular protective role of vitexicarpin isolated from Vitex rotundifolia in human umbilical vein endothelial cells. Inflammation. 35:584–593. 2012. View Article : Google Scholar : PubMed/NCBI
11	Kumar S, Sharma A, Madan B, Singhal V and Ghosh B: Isoliquiritigenin inhibits IKappaB kinase activity and ROS generation to block TNF-alpha induced expression of cell adhesion molecules on human endothelial cells. Biochem Pharmacol. 73:1602–1612. 2007. View Article : Google Scholar : PubMed/NCBI
12	Kim SM, Oh EY, Lee JH, Nam DW, Lee SG, Lee JH, Kim SH, Shin BS and Ahn KS: Brassinin combined with capsaicin enhances apoptotic and anti-metastatic effects in PC-3 human prostate cancer cells. Phytother Res. 29:1828–1836. 2015. View Article : Google Scholar : PubMed/NCBI
13	Banerjee T, DuHadaway JB, Gaspari P, Sutanto-Ward E, Munn DH, Mellor AL, Malachowski WP, Prendergast GC and Muller AJ: A key in vivo antitumor mechanism of action of natural product-based brassinins is inhibition of indoleamine 2,3-dioxygenase. Oncogene. 27:2851–2857. 2008. View Article : Google Scholar : PubMed/NCBI
14	Kello M, Drutovic D, Chripkova M, Pilatova M, Budovska M, Kulikova L, Urdzik P and Mojzis J: Ros-dependent antiproliferative effect of brassinin derivative hombrassinin in human colorectal cancer Caco2 cells. Molecules. 19:10877–10897. 2014. View Article : Google Scholar : PubMed/NCBI
15	Schneider CA, Rasband WS and Eliceiri KW: NIH Image to ImageJ: 25 years of image analysis. Nat Methods. 9:671–675. 2012. View Article : Google Scholar : PubMed/NCBI
16	Mackman N, Brand K and Edgington TS: Lipopolysaccharide mediated transcriptional activation of the human tissue factor gene in THP-1 cells requires both activator protein-1 and nuclear factor kappa B binding sites. J Exp Med. 174:1517–1526. 1991. View Article : Google Scholar : PubMed/NCBI
17	Bourdillon MC, Poston RN, Covacho C, Chignier E, Bricca G and McGregor JL: ICAM-1 deficiency reduces atherosclerotic lesions in double-knockout mice (ApoE(−/-)/ICAM-1(−/-)) fed a fat or a chow diet. Arterioscler Thromb Vasc Biol. 20:2630–2635. 2000. View Article : Google Scholar : PubMed/NCBI
18	Dustin ML, Rothlein R, Bhan AK, Dinarello CA and Springer TA: Induction by IL 1 and interferion-gamma: Tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1). J Immunol. 137:245–254. 1986.PubMed/NCBI
19	Bradley JR: TNF-mediated inflammatory disease. J Pathol. 214:149–160. 2008. View Article : Google Scholar : PubMed/NCBI
20	Hattori Y, Kasai K and Gross SS: NO suppresses while peroxynitrite sustains NF-kappaB: A paradigm to rationalize cytoprotective and cytotoxic actions attributed to NO. Cardiovasc Res. 63:31–40. 2004. View Article : Google Scholar : PubMed/NCBI
21	Brasier AR: The nuclear factor-kappaB-interleukin-6 signalling pathway mediating vascular inflammation. Cardiovasc Res. 86:211–218. 2010. View Article : Google Scholar : PubMed/NCBI
22	Simonini A, Moscucci M, Muller DW, Bates ER, Pagani FD, Burdick MD and Strieter RM: IL-8 is an angiogenic factor in human coronary atherectomy tissue. Circulation. 101:1519–1526. 2000. View Article : Google Scholar : PubMed/NCBI
23	Victor VM, Rocha M, Solá E, Bañuls C, Garcia-Malpartida K and Hernández-Mijares A: Oxidative stress, endothelial dysfunction and atherosclerosis. Curr Pharm Des. 15:2988–3002. 2009. View Article : Google Scholar : PubMed/NCBI