Aberrant promoter methylation of multiple genes in VSMC proliferation induced by Hcy

  • Authors:
    • Sheng‑Chao Ma
    • Jian‑Cheng Cao
    • Hui‑Ping Zhang
    • Yun Jiao
    • Hui Zhang
    • Yang‑Yang He
    • Yan‑Hua Wang
    • Xiao‑Ling Yang
    • An‑Ning Yang
    • Jue Tian
    • Ming‑Hao Zhang
    • Xiao‑Ming Yang
    • Guan‑Jun Lu
    • Shao‑Ju Jin
    • Yue‑Xia Jia
    • Yi‑Deng Jiang
  • View Affiliations

  • Published online on: September 19, 2017     https://doi.org/10.3892/mmr.2017.7521
  • Pages: 7775-7783
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Abstract

Vascular smooth muscle cell (VSMC) proliferation is a primary pathological event in atherosclerosis (AS), and homocysteine (Hcy) is an independent risk factor for AS. However, the underlying mechanisms are still lagging. Studies have used the combination of methylation of promoters of multiple genes to diagnose tumors, thus the aim of the current study was to investigate the role of methylation status of several genes in VSMCs treated with Hcy. CpG islands were identified in the promoters of platelet‑derived growth factor (PDGF), p53, phosphatase and tensin homologue on chromosome 10 (PTEN) and mitofusin 2 (MFN2). Hypomethylation was observed to occur in the promoter region of PDGF, hypermethylation in p53, PTEN and MFN2, and hypomethylation in two global methylation indicators, aluminium (Alu) and long interspersed nucleotide element‑1 (Line‑1). This was accompanied by an increase in the expression of PDGF, and reductions of p53, PTEN and MFN2, both in mRNA and protein levels. An elevation of S‑adenosylmethionine (SAM) and a reduction of S‑adenosylhomocysteine (SAH) and the SAM/SAH ratio were also identified. In conclusion, Hcy impacted methylation the of AS‑associated genes and global methylation status that mediate the cell proliferation, which may be a character of VSMCs treated with Hcy. The data provided evidence for mechanisms of VSMCs proliferation in AS induced by Hcy and may provide a new perspective for AS induced by Hcy.
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November-2017
Volume 16 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Ma SC, Cao JC, Zhang HP, Jiao Y, Zhang H, He YY, Wang YH, Yang XL, Yang AN, Tian J, Tian J, et al: Aberrant promoter methylation of multiple genes in VSMC proliferation induced by Hcy. Mol Med Rep 16: 7775-7783, 2017
APA
Ma, S., Cao, J., Zhang, H., Jiao, Y., Zhang, H., He, Y. ... Jiang, Y. (2017). Aberrant promoter methylation of multiple genes in VSMC proliferation induced by Hcy. Molecular Medicine Reports, 16, 7775-7783. https://doi.org/10.3892/mmr.2017.7521
MLA
Ma, S., Cao, J., Zhang, H., Jiao, Y., Zhang, H., He, Y., Wang, Y., Yang, X., Yang, A., Tian, J., Zhang, M., Yang, X., Lu, G., Jin, S., Jia, Y., Jiang, Y."Aberrant promoter methylation of multiple genes in VSMC proliferation induced by Hcy". Molecular Medicine Reports 16.5 (2017): 7775-7783.
Chicago
Ma, S., Cao, J., Zhang, H., Jiao, Y., Zhang, H., He, Y., Wang, Y., Yang, X., Yang, A., Tian, J., Zhang, M., Yang, X., Lu, G., Jin, S., Jia, Y., Jiang, Y."Aberrant promoter methylation of multiple genes in VSMC proliferation induced by Hcy". Molecular Medicine Reports 16, no. 5 (2017): 7775-7783. https://doi.org/10.3892/mmr.2017.7521