Baicalein protects tert‑butyl hydroperoxide‑induced hepatotoxicity dependent of reactive oxygen species removal

  • Authors:
    • Ya‑Fang Wang
    • Zheng‑Hai Tang
    • Ting Li
    • Xiao‑Huang Xu
    • Xiuping Chen
    • Ying Wang
    • Yi‑Tao Wang
    • Jin‑Jian Lu
  • View Affiliations

  • Published online on: September 22, 2017     https://doi.org/10.3892/mmr.2017.7592
  • Pages:8392-8398
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Abstract

Baicalein (BA), one of the major bioactive flavonoids isolated from Scutellariae Radix, possesses various pharmacological activities. The present study aimed to investigate the protective effects of BA on tert‑butyl hydroperoxide (t‑BHP)‑induced hepatotoxicity, and to investigate the potential mechanisms in LO2 cells. BA was demonstrated to possess protective properties against t‑BHP injury in LO2 cells, as evidenced by MTT and lactate dehydrogenase assays. BA significantly prevented t‑BHP‑induced depolarization of mitochondrial membrane potential (MMP), decreased the percentage of apoptotic cells caused by t‑BHP, and prevented intracellular reactive oxygen species (ROS) generation in LO2 cells. Furthermore, BA slightly triggered autophagy in LO2 cells, as evidenced by the elevation of LC3‑II expression, while BA combined treatment with an autophagy inhibitor (chloroquine) or activator (rapamycin) did not alter the hepatoprotective properties. In conclusion, BA may possess a hepatoprotective effect against t‑BHP‑induced liver cell injury, dependent on ROS removal. Therefore, BA may represent a potential drug candidate in protecting hepatotoxicity.

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December 2017
Volume 16 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

2016 Impact Factor: 1.692
Ranked #19/128 Medicine Research and Experimental
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APA
Wang, Y., Tang, Z., Li, T., Xu, X., Chen, X., Wang, Y. ... Lu, J. (2017). Baicalein protects tert‑butyl hydroperoxide‑induced hepatotoxicity dependent of reactive oxygen species removal. Molecular Medicine Reports, 16, 8392-8398. https://doi.org/10.3892/mmr.2017.7592
MLA
Wang, Y., Tang, Z., Li, T., Xu, X., Chen, X., Wang, Y., Wang, Y., Lu, J."Baicalein protects tert‑butyl hydroperoxide‑induced hepatotoxicity dependent of reactive oxygen species removal". Molecular Medicine Reports 16.6 (2017): 8392-8398.
Chicago
Wang, Y., Tang, Z., Li, T., Xu, X., Chen, X., Wang, Y., Wang, Y., Lu, J."Baicalein protects tert‑butyl hydroperoxide‑induced hepatotoxicity dependent of reactive oxygen species removal". Molecular Medicine Reports 16, no. 6 (2017): 8392-8398. https://doi.org/10.3892/mmr.2017.7592