MicroRNA‑24‑2 is associated with cell proliferation, invasion, migration and apoptosis in renal cell carcinoma

  • Authors:
    • Lu Jin
    • Yifan Li
    • Liping Nie
    • Tao He
    • Jia Hu
    • Jiaju Liu
    • Mingwei Chen
    • Min Shi
    • Zhimao Jiang
    • Yaoting Gui
    • Shangqi Yang
    • Yongqing Lai
  • View Affiliations

  • Published online on: October 4, 2017     https://doi.org/10.3892/mmr.2017.7705
  • Pages:9157-9164
0

Abstract

Micro (mi)RNAs are involved in multiple cellular processes, and alterations in miRNA expression have been demonstrated to lead to tumorigenesis. Previous microarray analysis revealed that miRNA (miR)‑24 was downregulated in renal cell carcinoma (RCC). Additionally, miR‑24 has been identified as an oncogene and tumor suppressor in various cancers. The present study assessed the expression levels of two stem‑loops of miR‑24, miR‑24‑1 and miR‑24‑2, in RCC tissues and paired healthy tissues by reverse transcription‑quantitative polymerase chain reaction. The results revealed that miR‑24‑2 was upregulated in RCC tissues and ACHN, 786‑O and 769P cell lines compared with healthy tissues and HEK‑293T cells, respectively, whereas miR‑24‑1 was almost absent in RCC and healthy kidney tissues. To investigate the role of miR‑24‑2 in RCC, a synthesized miR‑24‑2 mimic, negative control (NC), inhibitor or inhibitor NC was transfected into 786‑O and ACHN RCC cells, and cell proliferation, mobility and apoptosis assays were performed. The results of the present study revealed that miR‑24‑2 was associated with cell proliferation, migration, invasion and apoptosis, thus demonstrating that miR‑24‑2 may serve a role as an oncogene in RCC. Further studies are required to investigate the signaling pathways of miR‑24‑2, and the potential of miR‑24‑2 as a therapeutic target or biomarker for the early detection of RCC.

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December 2017
Volume 16 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

2016 Impact Factor: 1.692
Ranked #19/128 Medicine Research and Experimental
(total number of cites)

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APA
Jin, L., Li, Y., Nie, L., He, T., Hu, J., Liu, J. ... Lai, Y. (2017). MicroRNA‑24‑2 is associated with cell proliferation, invasion, migration and apoptosis in renal cell carcinoma. Molecular Medicine Reports, 16, 9157-9164. https://doi.org/10.3892/mmr.2017.7705
MLA
Jin, L., Li, Y., Nie, L., He, T., Hu, J., Liu, J., Chen, M., Shi, M., Jiang, Z., Gui, Y., Yang, S., Lai, Y."MicroRNA‑24‑2 is associated with cell proliferation, invasion, migration and apoptosis in renal cell carcinoma". Molecular Medicine Reports 16.6 (2017): 9157-9164.
Chicago
Jin, L., Li, Y., Nie, L., He, T., Hu, J., Liu, J., Chen, M., Shi, M., Jiang, Z., Gui, Y., Yang, S., Lai, Y."MicroRNA‑24‑2 is associated with cell proliferation, invasion, migration and apoptosis in renal cell carcinoma". Molecular Medicine Reports 16, no. 6 (2017): 9157-9164. https://doi.org/10.3892/mmr.2017.7705