Epithelial‑mesenchymal transition in colorectal carcinoma cells is mediated by DEK/IMP3

  • Authors:
    • Shuping You
    • Yun Guan
    • Weihong Li
  • View Affiliations

  • Published online on: November 3, 2017     https://doi.org/10.3892/mmr.2017.7943
  • Pages: 1065-1070
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Abstract

To investigate the inhibitory effects of DEK/insulin‑like growth factor II mRNA binding protein 3 (IMP3) on epithelial‑mesenchymal transition (EMT) in colorectal carcinoma cells. SW620 and SW480 cell lines were selected. DEK‑interfering lentivirus was transfected to knockdown DEK expression. Subsequently, MTT assays and flow cytometry were utilized to measure cell viability, and apoptosis, respectively. Cell invasion was detected using a Transwell assay. Quantitative polymerase chain reaction and western blot analysis were used to detect the expression of E‑cadherin, vimentin, and matrix metalloproteinase (MMP)‑9. Compared with the blank control, cells transfected with DEK‑interfering lentivirus demonstrated a remarkable reduction in cell viability (P<0.05). The apoptotic rate in the DEK‑interfering lentivirus group was significantly enhanced compared with the blank control group (P<0.05). In the DEK‑interfering lentivirus group, the expression of E‑cadherin was significantly elevated (P<0.05), while the expression of vimentin and MMP‑9 were significantly reduced in both cell lines (P<0.05). The results of the present study demonstrated that EMT of colorectal carcinoma cells was partially mediated by DEK, which likely affected the invasive ability of colorectal carcinoma cells. In addition, cell proliferation and apoptosis were susceptible to DEK silencing. The current study has provided experimental evidence for the treatment of colorectal carcinoma using DEK silencing.
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January-2018
Volume 17 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
You S, Guan Y and Li W: Epithelial‑mesenchymal transition in colorectal carcinoma cells is mediated by DEK/IMP3. Mol Med Rep 17: 1065-1070, 2018
APA
You, S., Guan, Y., & Li, W. (2018). Epithelial‑mesenchymal transition in colorectal carcinoma cells is mediated by DEK/IMP3. Molecular Medicine Reports, 17, 1065-1070. https://doi.org/10.3892/mmr.2017.7943
MLA
You, S., Guan, Y., Li, W."Epithelial‑mesenchymal transition in colorectal carcinoma cells is mediated by DEK/IMP3". Molecular Medicine Reports 17.1 (2018): 1065-1070.
Chicago
You, S., Guan, Y., Li, W."Epithelial‑mesenchymal transition in colorectal carcinoma cells is mediated by DEK/IMP3". Molecular Medicine Reports 17, no. 1 (2018): 1065-1070. https://doi.org/10.3892/mmr.2017.7943