Oxymatrine induces A549 human non‑small lung cancer cell apoptosis via extrinsic and intrinsic pathways

Zhou,Guang‑Zhou; Shi,Yan‑Yan; Cui,Liu‑Su; Li,A‑Fang; Wang,Qing‑Qing; Liu,Min

doi:10.3892/mmr.2017.7982

Oxymatrine induces A549 human non‑small lung cancer cell apoptosis via extrinsic and intrinsic pathways

Authors:
- Guang‑Zhou Zhou
- Yan‑Yan Shi
- Liu‑Su Cui
- A‑Fang Li
- Qing‑Qing Wang
- Min Liu
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Affiliations: Department of Biotechnology, College of Bioengineering, Henan University of Technology, Zhengzhou, Henan 450001, P.R. China, Laboratory of Morphology, College of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Infectious Diseases, The Xiang'an Hospital of Xiamen University, Xiamen, Fujian 361005, P.R. China
Published online on: November 6, 2017 https://doi.org/10.3892/mmr.2017.7982
Pages: 1071-1076

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Abstract

Oxymatrine is one of the primary natural compounds extracted from the Sophora flavescens, and has been reported to exhibit numerous pharmacological properties including cancer‑preventive and anti‑cancer effects, however the mechanisms as to how oxymatrine exhibits anti‑proliferative activity in non‑small cell lung carcinoma cells remains uncertain. The present study aimed to explore the mechanism of its anti‑cancer effect, and whether it is due to apoptosis induction and anti‑migration in the A549 lung cancer cell line. Detection of morphological alterations, MTT analysis, Hoechst/propidium iodide dual staining and terminal deoxynucleotidyl transferase dUTP nick end labeling assays verified that oxymatrine induced A549 cell apoptosis. The caspase pan‑inhibitor z‑VAD‑FMK resulted in disappearance of oxymatrine‑elicited nuclei fragmentation via Hoechst 33342 staining. JC‑1 staining demonstrated a decrease in mitochondrial membrane potential which further verified the induction of apoptosis by oxymatrine. The caspase‑3, 8 and 9 activities of oxymatrine‑treated cells were activated, which suggested that extrinsic and intrinsic apoptotic pathways were involved in the anti‑proliferative effects of oxymatrine in A549 cells. Furthermore, the wound healing assay verified the anti‑migratory effects of oxymatrine in A549 cells.

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