Open Access

Preparation and characterization of acellular adipose tissue matrix using a combination of physical and chemical treatments

  • Authors:
    • Mei Song
    • Yi Liu
    • Ling Hui
  • View Affiliations

  • Published online on: October 24, 2017     https://doi.org/10.3892/mmr.2017.7857
  • Pages:138-146
  • Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Decellularized adipose extracellular matrix (ECM) has been used in the clinic to support the regeneration of adipose tissues. The methods used to produce adipose tissue ECM scaffolds exhibit distinct effects upon the structural and functional components of the resultant scaffold material. The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion. Cellular components, including nucleic acids were effectively removed without significant disruption of the morphology or structure of the ECM. The compositions of major ECM components were evaluated, including acid/pepsin soluble collagen, sulfated glycosaminoglycan and laminin. The decellularized ECM exhibited satisfactory mechanical properties. Cell seeding experiments involving human adipose‑derived stem cells indicated that the decellularized ECM provided an inductive microenvironment for adipogenesis without the need for exogenous differentiation factors. Higher levels of glycerol‑3‑phosphate dehydrogenase activity were observed among induced cells in the ECM scaffolds when compared with induced cells in collagen type I scaffolds. In conclusion, the results suggested that the decellularized ECM, containing biological and chemical cues of native human ECM, may be an ideal scaffold material for autologous and allograft tissue engineering.

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January 2018
Volume 17 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

2016 Impact Factor: 1.692
Ranked #19/128 Medicine Research and Experimental
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APA
Song, M., Liu, Y., & Hui, L. (2018). Preparation and characterization of acellular adipose tissue matrix using a combination of physical and chemical treatments. Molecular Medicine Reports, 17, 138-146. https://doi.org/10.3892/mmr.2017.7857
MLA
Song, M., Liu, Y., Hui, L."Preparation and characterization of acellular adipose tissue matrix using a combination of physical and chemical treatments". Molecular Medicine Reports 17.1 (2018): 138-146.
Chicago
Song, M., Liu, Y., Hui, L."Preparation and characterization of acellular adipose tissue matrix using a combination of physical and chemical treatments". Molecular Medicine Reports 17, no. 1 (2018): 138-146. https://doi.org/10.3892/mmr.2017.7857