Preparation and characterization of acellular adipose tissue matrix using a combination of physical and chemical treatments

Song,Mei; Liu,Yi; Hui,Ling

doi:10.3892/mmr.2017.7857

Preparation and characterization of acellular adipose tissue matrix using a combination of physical and chemical treatments

Authors:
- Mei Song
- Yi Liu
- Ling Hui
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Affiliations: Burns and Plastic Surgery Center, General Hospital of Lanzhou Military Command of The People's Liberation Army, Institute of Orthopedics of Gansu Province, Lanzhou, Gansu 730050, P.R. China, Department of Clinical Laboratories, General Hospital of Lanzhou Military Command of The People's Liberation Army, Key Laboratory of Stem Cells and Gene Medicine of Gansu Province, Lanzhou, Gansu 730050, P.R. China
Published online on: October 24, 2017 https://doi.org/10.3892/mmr.2017.7857
Pages: 138-146
Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Decellularized adipose extracellular matrix (ECM) has been used in the clinic to support the regeneration of adipose tissues. The methods used to produce adipose tissue ECM scaffolds exhibit distinct effects upon the structural and functional components of the resultant scaffold material. The current study presents an acellular ECM scaffold from human adipose tissues derived using successive physical and chemical treatments, including repeated freeze‑thaw cycles followed by centrifugation, polar solvent extraction and enzymatic digestion. Cellular components, including nucleic acids were effectively removed without significant disruption of the morphology or structure of the ECM. The compositions of major ECM components were evaluated, including acid/pepsin soluble collagen, sulfated glycosaminoglycan and laminin. The decellularized ECM exhibited satisfactory mechanical properties. Cell seeding experiments involving human adipose‑derived stem cells indicated that the decellularized ECM provided an inductive microenvironment for adipogenesis without the need for exogenous differentiation factors. Higher levels of glycerol‑3‑phosphate dehydrogenase activity were observed among induced cells in the ECM scaffolds when compared with induced cells in collagen type I scaffolds. In conclusion, the results suggested that the decellularized ECM, containing biological and chemical cues of native human ECM, may be an ideal scaffold material for autologous and allograft tissue engineering.

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