Homology modeling and prediction of B‑cell and T‑cell epitopes of the house dust mite allergen Der f 20

Teng,Feixiang; Yu,Lili; Sun,Jinxia; Wang,Nan; Cui,Yubao

doi:10.3892/mmr.2017.8066

Homology modeling and prediction of B‑cell and T‑cell epitopes of the house dust mite allergen Der f 20

Authors:
- Feixiang Teng
- Lili Yu
- Jinxia Sun
- Nan Wang
- Yubao Cui
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Affiliations: Department of Basic Medicine, Jiangsu Vocational College of Medicine, Yancheng, Jiangsu 224005, P.R. China, Department of Clinical Laboratory, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214023, P.R. China
Published online on: November 15, 2017 https://doi.org/10.3892/mmr.2017.8066
Pages: 1807-1812

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Abstract

House dust mite allergens can cause allergic diseases, including asthma, atopic dermatitis and rhinitis. Der f 20 is a novel allergen of Dermatophagoides farina (Der f), which is an arginine kinase. In the present study, the B‑cell and T‑cell epitopes of Der f 20 were predicted. The protein attribution, patterns, physicochemical properties and secondary structure of Der f 20 were also predicted. Der f 20 is a member of the ATP:guanido phosphotransferase family and contains a phosphagen kinase pattern. Using homology modeling, the present study constructed a reasonable tertiary structure of Der f 20. Using BcePred, ABCpred, BCPred and BPAP systems, B‑cell epitopes at 20‑25, 41‑49, 111‑118, 131‑141, 170‑174 and 312‑321 were predicted. Using NetMHCIIpan‑3.0 and NetMHCII‑2.2, T‑cell epitopes were predicted at 194‑202, 239‑247 and 274‑282. These results provide a theoretical basis for the design off Der f 20 epitope‑based vaccines.

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