Cellular properties of the fermented microalgae Pavlova lutheri and its isolated active peptide in osteoblastic differentiation of MG‑63 cells

Qian,Zhong‑Ji; Ryu,Bomi; Kang,Kyong‑Hwa; Heo,Soo‑Jin; Kang,Do‑Hyung; Bae,Sung  Yong; Park,Sun‑Joo; Kim,Jae‑Il; Kim,Young‑Mog; Kim,Yong‑Tae; Jung,Won‑Kyo

doi:10.3892/mmr.2017.8087

Cellular properties of the fermented microalgae Pavlova lutheri and its isolated active peptide in osteoblastic differentiation of MG‑63 cells

Authors:
- Zhong‑Ji Qian
- Bomi Ryu
- Kyong‑Hwa Kang
- Soo‑Jin Heo
- Do‑Hyung Kang
- Sung Yong Bae
- Sun‑Joo Park
- Jae‑Il Kim
- Young‑Mog Kim
- Yong‑Tae Kim
- Won‑Kyo Jung
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Affiliations: Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, Guangdong 524088, P.R. China, School of Pharmacy, The University of Queensland, Brisbane, QLD 4072, Australia, Marine Bioprocess Research Center, Pukyong National University, Busan 48513, Republic of Korea, Jeju International Marine Science Center for Research and Education, Korea Institute of Ocean Science and Technology, Jeju 63349, Republic of Korea, Department of Naval Architecture and Marine Systems Engineering, Pukyong National University, Busan 48513, Republic of Korea, Department of Chemistry, Pukyong National University, Busan 48513, Republic of Korea, Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea, Department of Food Science and Technology, Pukyong National University, Busan 48513, Republic of Korea, Department of Food Science and Biotechnology, Kunsan National University, Kunsan, North Jeolla 573‑701, Republic of Korea, Department of Biomedical Engineering, and Center for Marine‑Integrated Biomedical Technology (BK21 Plus) and Marine‑Integrated Bionics Research Center, Pukyong National University, Busan 48513, Republic of Korea
Published online on: November 15, 2017 https://doi.org/10.3892/mmr.2017.8087
Pages: 2044-2050

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Abstract

Fermented microalgae Pavlova lutheri (P. lutheri), the product of Hansenula polymorpha fermentation, exhibited an increase in alkaline phosphatase (ALP) activity in MG‑63 osteoblastic cells when compared to that of non‑fermented P. lutheri. Fractionation of the fermented P. lutheri resulted in identification of the active peptide [peptide of P. lutheri fermentation (PPLF)] with the sequence of EPQWFL. PPLF significantly increased ALP release from MG‑63 cells and mineralization in a dose‑dependent manner. In addition, the intracellular levels of ALP and osteocalcin (OCN) proteins were augmented by PPLF treatment. To identify the molecular mechanism underlying the effect of PPLF on osteoblastic differentiation, the phosphorylation levels of the mitogen‑activated protein kinases, p38, extracellular signal‑regulated kinases 1/2 and Jun, and nuclear factor (NF)‑κB were determined following PPLF treatment and the differences in expression were analyzed using p38 and NF‑κB selective inhibitors. These results concluded that PPLF from fermented P. lutheri induced osteoblastic differentiation by increasing ALP and OCN release in MG‑63 cells via the p38/p65 signaling pathway, indicating that PPLF supplement may be effective for therapeutic application in the field of bone health.

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