Effects of long‑term scopolamine treatment on cognitive deficits and calcium binding proteins immunoreactivities in the mouse hippocampus

Ahn,Ji Hyeon; Chen,Bai Hui; Yan,Bing Chun; Park,Joon Ha; Kang,Il Jun; Lee,Tae‑Kyeong; Cho,Jeong Hwi; Shin,Bich‑Na; Lee,Jae‑Chul; Jeon,Yong Hwan; Hong,Seongkweon; Lee,Young Joo; Choi,Soo Young; Won,Moo‑Ho

doi:10.3892/mmr.2017.7928

Effects of long‑term scopolamine treatment on cognitive deficits and calcium binding proteins immunoreactivities in the mouse hippocampus

Authors:
- Ji Hyeon Ahn
- Bai Hui Chen
- Bing Chun Yan
- Joon Ha Park
- Il Jun Kang
- Tae‑Kyeong Lee
- Jeong Hwi Cho
- Bich‑Na Shin
- Jae‑Chul Lee
- Yong Hwan Jeon
- Seongkweon Hong
- Young Joo Lee
- Soo Young Choi
- Moo‑Ho Won
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Affiliations: Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea, Department of Histology and Embryology, Institute of Neuroscience, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China, Department of Traditional Chinese and Western Medicine, Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, Jiangsu 225001, P.R. China, Department of Food Science and Nutrition, Hallym University, Chuncheon 24252, Republic of Korea, Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea, Department of Radiology, School of Medicine, Kangwon National University, Kangwon National University Hospital, Chuncheon 24341, Republic of Korea, Department of Surgery, School of Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea, Department of Emergency Medicine, Seoul Hospital, College of Medicine, Sooncheonhyang University, Seoul 04401, Republic of Korea
Published online on: October 27, 2017 https://doi.org/10.3892/mmr.2017.7928
Pages: 293-299
Copyright: © Ahn et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

GABAergic projections terminate on numerous hippocampal interneurons containing calcium binding proteins (CBPs), including calbindin D‑28k (CB), calretinin (CR) and parvalbumin (PV). Memory deficits and expression levels of CB, CR, and PV were examined in the hippocampal subregions following systemic scopolamine (Scop; 1 mg/kg) treatment for 4 weeks in mice. Scop treatment induced significant memory deficits from 1 week after Scop treatment. CB, CR and PV immunoreactivities distributions were in hippocampal subregions [CA1 and CA3 regions, and the dentate gyrus (DG)]. CB immunoreactivity (CB+) was gradually decreased in all subregions until 2 weeks after Scop treatment, and CB+ was decreased to the lowest level in all subregions at 3 and 4 weeks. CR+ in the CA1 region was gradually decreased until 2 weeks and hardly observed at 3 and 4 weeks; in the CA3 region, CR+ was not altered in all subregions at any time. In the DG, CR+ was gradually decreased until 2 weeks and lowest at 3 and 4 weeks. PV+ in the CA1 region was not altered at 1 week, and gradually decreased from 2 weeks. In the CA3 region, PV+ did not change in any subregions at any time. In the DG, PV+ was not altered at 1 week, decreased at 2 weeks, and lowest at 3 and 4 weeks. In brief, Scop significantly decreased CBPs expressions in the hippocampus ≥3 weeks after the treatment although memory deficits had developed at 1 week. Therefore, it is suggested that Scop (1 mg/kg) must be systemically treated for ≥3 weeks to investigate changes in expression levels of CBPs in the hippocampus.

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