Preparation of chitosan‑silicon dioxide/BCSG1‑siRNA nanoparticles to enhance therapeutic efficacy in breast cancer cells

  • Authors:
    • Lan Cui
    • Ruifeng Zheng
    • Wentao Liu
    • Peihong Shen
    • Youcai Tang
    • Junbo Luo
    • Wei Zhang
    • Guocong Jia
    • Yongfeng Wang
    • Shuaihua Zhao
    • Qiaoting Xie
    • Yanwei Li
  • View Affiliations

  • Published online on: October 26, 2017     https://doi.org/10.3892/mmr.2017.7887
  • Pages: 436-441
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Abstract

Breast cancer is one of the most serious diseases, posing threats to women's physical and mental health. Gene therapy has been gradually regarded as an important part of tumor therapeutics. In the present study, the breast cancer‑specific gene 1‑small interference RNA (BCSG1‑siRNA) plasmid was designed, then encapsulated by chitosan‑silicon dioxide nanometer carriers. The results demonstrated a successful encapsulation of BCSG1‑siRNA in chitosan‑silicon dioxide nanoparticles (encapsulation efficiency exceeded 90%). BCSG1‑siRNA was released slowly (the release rate was almost 30% after 24 h). The cytotoxic effect on MCF‑7 cells was enhanced by increasing the concentration of nanoparticle (the proliferation rate was reduced to 13.4±5.3% and apoptosis rate was increased to 71.5±6.8%). Therefore, the materials presented in the current study acted as successful gene carriers and exhibited significant antitumor effects in breast cancer cells.
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January-2018
Volume 17 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Cui L, Zheng R, Liu W, Shen P, Tang Y, Luo J, Zhang W, Jia G, Wang Y, Zhao S, Zhao S, et al: Preparation of chitosan‑silicon dioxide/BCSG1‑siRNA nanoparticles to enhance therapeutic efficacy in breast cancer cells. Mol Med Rep 17: 436-441, 2018
APA
Cui, L., Zheng, R., Liu, W., Shen, P., Tang, Y., Luo, J. ... Li, Y. (2018). Preparation of chitosan‑silicon dioxide/BCSG1‑siRNA nanoparticles to enhance therapeutic efficacy in breast cancer cells. Molecular Medicine Reports, 17, 436-441. https://doi.org/10.3892/mmr.2017.7887
MLA
Cui, L., Zheng, R., Liu, W., Shen, P., Tang, Y., Luo, J., Zhang, W., Jia, G., Wang, Y., Zhao, S., Xie, Q., Li, Y."Preparation of chitosan‑silicon dioxide/BCSG1‑siRNA nanoparticles to enhance therapeutic efficacy in breast cancer cells". Molecular Medicine Reports 17.1 (2018): 436-441.
Chicago
Cui, L., Zheng, R., Liu, W., Shen, P., Tang, Y., Luo, J., Zhang, W., Jia, G., Wang, Y., Zhao, S., Xie, Q., Li, Y."Preparation of chitosan‑silicon dioxide/BCSG1‑siRNA nanoparticles to enhance therapeutic efficacy in breast cancer cells". Molecular Medicine Reports 17, no. 1 (2018): 436-441. https://doi.org/10.3892/mmr.2017.7887