Open Access

MicroRNA‑103 regulates tumorigenesis in colorectal cancer by targeting ZO‑1

  • Authors:
    • Jin Ke
    • Weiwei Shao
    • Yasu Jiang
    • Junfei Xu
    • Feng Li
    • Jun Qin
  • View Affiliations

  • Published online on: November 7, 2017     https://doi.org/10.3892/mmr.2017.8007
  • Pages: 783-788
  • Copyright: © Ke et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Given the emerging role of microRNAs (miRs) in cancer progression, the present study investigated the role and underlying mechanism of miR‑103 in colorectal cancer (CRC). Reverse transcription‑quantitative polymerase chain reaction was conducted to quantify the expression levels of miR‑103 in clinical specimens and cell lines. The role of miR‑103 in CRC was examined using MTT, colony formation and transwell assays. In addition, a luciferase reporter assay was used to confirm an associated between the 3' untranslated region of zonula occuldens‑1 (ZO‑1) and miR‑103. The results demonstrated that miR‑103 was upregulated in CRC. Overexpression of miR‑103 promoted CRC cell proliferation and migration in vitro, whereas downregulation of miR‑103 inhibited cell proliferation and migration. ZO‑1 was identified as a direct target of miR‑103, revealing its expression to be inversely correlated with miR‑103 expression in CRC samples. In conclusion, the present study revealed that miR‑103 has strong tumor‑promoting effects via of targeting ZO‑1 in CRC and has potential development of miRNA‑based targeted approaches for the treatment of CRC.
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January-2018
Volume 17 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Ke J, Shao W, Jiang Y, Xu J, Li F and Qin J: MicroRNA‑103 regulates tumorigenesis in colorectal cancer by targeting ZO‑1. Mol Med Rep 17: 783-788, 2018.
APA
Ke, J., Shao, W., Jiang, Y., Xu, J., Li, F., & Qin, J. (2018). MicroRNA‑103 regulates tumorigenesis in colorectal cancer by targeting ZO‑1. Molecular Medicine Reports, 17, 783-788. https://doi.org/10.3892/mmr.2017.8007
MLA
Ke, J., Shao, W., Jiang, Y., Xu, J., Li, F., Qin, J."MicroRNA‑103 regulates tumorigenesis in colorectal cancer by targeting ZO‑1". Molecular Medicine Reports 17.1 (2018): 783-788.
Chicago
Ke, J., Shao, W., Jiang, Y., Xu, J., Li, F., Qin, J."MicroRNA‑103 regulates tumorigenesis in colorectal cancer by targeting ZO‑1". Molecular Medicine Reports 17, no. 1 (2018): 783-788. https://doi.org/10.3892/mmr.2017.8007