Open Access

Downregulation of miR‑29b targets DNMT3b to suppress cellular apoptosis and enhance proliferation in pancreatic cancer

Retraction in: /10.3892/mmr.2023.13049

  • Authors:
    • Li‑Hua Wang
    • Ju Huang
    • Cheng‑Rong Wu
    • Liu‑Ye Huang
    • Jun Cui
    • Zhi‑Zhi Xing
    • Chun‑Yu Zhao
  • View Affiliations

  • Published online on: November 23, 2017     https://doi.org/10.3892/mmr.2017.8145
  • Pages: 2113-2120
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].

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Abstract

As one of the most aggressive types of tumor, pancreatic cancer is a principal cause of tumor‑associated mortality. Negative associations between microRNA‑29 (miR‑29) and DNA methyltransferases (DNMT) 3a and 3b have been demonstrated to be associated with the carcinogenesis of a number of types of cancer; however, this has not been completely elucidated in pancreatic cancer. In the present study, pancreatic cancer tissues (n=15) and corresponding paracancerous tissues (n=15) were obtained and the results of reverse transcription‑quantitative polymerase chain reaction analysis indicated decreased expression of miR‑29b and enhanced mRNA expression of DNMT3b in pancreatic cancer tissues, compared with the corresponding paracancerous tissues. Increased protein expression of DNMT3b was demonstrated by western blotting and immunohistochemistry. In addition, the negative association between miR‑29b and DNMT3b was noted in pancreatic cancer tissues, and luciferase reporter assays confirmed that miR‑29b was able to directly target DNMT3b in vitro. Notably, miR‑29b overexpression was able to decrease cell viability and to promote the apoptosis by targeting DNMT3b, and the knockdown of DNMT3b exhibited consistent results in vitro and in vivo. The results of the present study suggested that miR‑29b, as a tumor suppressor, may be a novel target for the development of treatments for pancreatic cancer.
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February-2018
Volume 17 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wang LH, Huang J, Wu CR, Huang LY, Cui J, Xing ZZ and Zhao CY: Downregulation of miR‑29b targets DNMT3b to suppress cellular apoptosis and enhance proliferation in pancreatic cancer Retraction in /10.3892/mmr.2023.13049. Mol Med Rep 17: 2113-2120, 2018
APA
Wang, L., Huang, J., Wu, C., Huang, L., Cui, J., Xing, Z., & Zhao, C. (2018). Downregulation of miR‑29b targets DNMT3b to suppress cellular apoptosis and enhance proliferation in pancreatic cancer Retraction in /10.3892/mmr.2023.13049. Molecular Medicine Reports, 17, 2113-2120. https://doi.org/10.3892/mmr.2017.8145
MLA
Wang, L., Huang, J., Wu, C., Huang, L., Cui, J., Xing, Z., Zhao, C."Downregulation of miR‑29b targets DNMT3b to suppress cellular apoptosis and enhance proliferation in pancreatic cancer Retraction in /10.3892/mmr.2023.13049". Molecular Medicine Reports 17.2 (2018): 2113-2120.
Chicago
Wang, L., Huang, J., Wu, C., Huang, L., Cui, J., Xing, Z., Zhao, C."Downregulation of miR‑29b targets DNMT3b to suppress cellular apoptosis and enhance proliferation in pancreatic cancer Retraction in /10.3892/mmr.2023.13049". Molecular Medicine Reports 17, no. 2 (2018): 2113-2120. https://doi.org/10.3892/mmr.2017.8145