Open Access

Integrative analysis of long non-coding RNAs and messenger RNA expression profiles in systemic lupus erythematosus

  • Authors:
    • Qing Luo
    • Xue Li
    • Chuxin Xu
    • Lulu Zeng
    • Jianqing Ye
    • Yang Guo
    • Zikun Huang
    • Junming Li
  • View Affiliations

  • Published online on: December 22, 2017     https://doi.org/10.3892/mmr.2017.8344
  • Pages: 3489-3496
  • Copyright: © Luo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Thousands of long noncoding RNAs (lncRNAs) have been reported and represent an important subset of pervasive genes associated with a broad range of biological functions. Abnormal expression levels of lncRNAs have been demonstrated in multiple types of human disease. However, the role of lncRNAs in systemic lupus erythematosus (SLE) remains poorly understood. In the present study, the expression patterns of lncRNAs and messenger RNAs (mRNAs) were investigated in peripheral blood mononuclear cells (PBMCs) in SLE using Human lncRNA Array v3.0 (8x60 K; Arraystar, Inc., Rockville, MD, USA). The microarray results indicated that 8,868 lncRNAs (3,657 upregulated and 5,211 downregulated) and 6,876 mRNAs (2,862 upregulated and 4,014 downregulated) were highly differentially expressed in SLE samples compared with the healthy group. Gene ontology (GO) analysis of lncRNA target prediction indicated the presence of 474 matched lncRNA‑mRNA pairs for 293 differentially expressed lncRNAs (fold change, ≥3.0) and 381 differentially expressed mRNAs (fold change, ≥3.0). The most enriched pathways were ‘Transcriptional misregulation in cancer’ and ‘Valine, leucine and isoleucine degradation’. Furthermore, reverse transcription‑quantitative polymerase chain reaction data verified six abnormal lncRNAs and mRNAs in SLE. The results indicate that the lncRNA expression profile in SLE was significantly changed. In addition, a range of SLE‑associated lncRNAs were identified. Thus, the present results provide important insights regarding lncRNAs in the pathogenesis of SLE.
View Figures
View References

Related Articles

Journal Cover

March-2018
Volume 17 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Luo Q, Li X, Xu C, Zeng L, Ye J, Guo Y, Huang Z and Li J: Integrative analysis of long non-coding RNAs and messenger RNA expression profiles in systemic lupus erythematosus. Mol Med Rep 17: 3489-3496, 2018
APA
Luo, Q., Li, X., Xu, C., Zeng, L., Ye, J., Guo, Y. ... Li, J. (2018). Integrative analysis of long non-coding RNAs and messenger RNA expression profiles in systemic lupus erythematosus. Molecular Medicine Reports, 17, 3489-3496. https://doi.org/10.3892/mmr.2017.8344
MLA
Luo, Q., Li, X., Xu, C., Zeng, L., Ye, J., Guo, Y., Huang, Z., Li, J."Integrative analysis of long non-coding RNAs and messenger RNA expression profiles in systemic lupus erythematosus". Molecular Medicine Reports 17.3 (2018): 3489-3496.
Chicago
Luo, Q., Li, X., Xu, C., Zeng, L., Ye, J., Guo, Y., Huang, Z., Li, J."Integrative analysis of long non-coding RNAs and messenger RNA expression profiles in systemic lupus erythematosus". Molecular Medicine Reports 17, no. 3 (2018): 3489-3496. https://doi.org/10.3892/mmr.2017.8344