Synthetic E-selectin prevents postoperative vascular restenosis by inhibiting nuclear factor κB in rats

Liu,Jiangang; Liu,Zhongjie; Hu,Xiaohui; Zhang,Yuan; Zhang,Shiming

doi:10.3892/mmr.2018.8536

Synthetic E-selectin prevents postoperative vascular restenosis by inhibiting nuclear factor κB in rats

Authors:
- Jiangang Liu
- Zhongjie Liu
- Xiaohui Hu
- Yuan Zhang
- Shiming Zhang
View Affiliations

Affiliations: Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China, Department of Neurosurgery, Shiqianxian People's Hospital, Guizhou 555100, P.R. China, Department of Radiology, Suzhou Guangji Hospital, Suzhou, Jiangsu 215006, P.R. China
Published online on: February 1, 2018 https://doi.org/10.3892/mmr.2018.8536
Pages: 5065-5073
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

During the development of postoperative vascular restenosis, the aberrant proliferation of vascular smooth muscle cells (VSMCs) is a critical event resulting in intimal hyperplasia. Inflammatory responses involving the activation of nuclear factor (NF)‑κB are among the major molecular processes underlying restenosis. The present study aimed to investigate the roles of NF‑κB in VSMC proliferation and restenosis following vascular anastomosis, as well as to evaluate the potential of synthetic E‑selectin to downregulate NF‑κB and thus inhibit vascular hyperplasia. A total of 72 adult male Sprague‑Dawley rats were randomly assigned to three groups: Control, operation and treatment groups. Rats in the operation and treatment groups received longitudinal incisions in the right carotid arteries, which were closed using interrupted sutures. Following vascular anastomosis, synthetic E‑selectin (10 mg/kg), or an equal volume of saline, was immediately injected into the right femoral vein of rats in the treatment and operation groups, respectively. Following surgery, the mRNA and protein expression levels of NF‑κB at the site of anastomosis, the levels of tumor necrosis factor‑α and interleukin‑6 in the serum, NF‑κB binding activity, and the presence of proliferating cell nuclear antigen (PCNA)‑positive cells were evaluated by western blotting, reverse transcription‑quantitative polymerase chain reaction, ELISA, electrophoretic mobility shift assay and immunofluorescence staining. The present results demonstrated that following treatment with synthetic E‑selectin, the levels of NF‑κB and the inflammatory response, as well as the presence of PCNA‑positive cells, were significantly reduced (P<0.01). In conclusion, the results of the present study suggested that synthetic E‑selectin may exert anti‑inflammatory and anti‑restenotic effects following vascular anastomosis in vivo.

View Figures

View References

1	Jia Q, Liu LP and Wang YJ: Stroke in China. Clin Exp Pharmacol Physiol. 37:259–264. 2010. View Article : Google Scholar : PubMed/NCBI
2	Kim HJ, Kim SK, Park HJ, Chung JH, Chun J, Yun DH and Kim YO: Polymorphisms of IGFI contribute to the development of ischemic stroke. Exp Ther Med. 3:93–98. 2012. View Article : Google Scholar : PubMed/NCBI
3	Chaturvedi S, Bruno A, Feasby T, Holloway R, Benavente O, Cohen SN, Cote R, Hess D, Saver J, Spence JD, et al: Carotid endarterectomy-an evidence-based review: Report of the therepeutics and technology assessment subcommittee of the american academy of neurology. Neurology. 65:794–801. 2005. View Article : Google Scholar : PubMed/NCBI
4	Matter CM, Ma L, von Lukowicz T, Meier P, Lohmann C, Zhang D, Kilic U, Hofmann E, Ha SW, Hersberger M, et al: Increased balloon-induced inflammation, proliferation, and neointima formation in apolipoprotein E (ApoE) knockout mice. Stroke. 37:2625–2632. 2006. View Article : Google Scholar : PubMed/NCBI
5	Arguizan C, Tringuart L, Touboul PJ, Long A, Feasson S, Terriat B, Gobin-Metteil MP, Guidolin B, Cohen S and Mas JL: EVA-3S Investigators: Restenosis is more frequent after carotid stenting than after endarterectomy: The EVA-3S study. Stroke. 42:1015–1020. 2011. View Article : Google Scholar : PubMed/NCBI
6	Matter CM, Chadjichristos CE, Meier P, von Lukowicz T, Lohmann C, Schuler PK, Zhang D, Odermatt B, Hofmann E, Brunner T, et al: Role of endogenous Fas (CD95/Apo-1) ligand in balloon-induced apoptosis, inflammation, and neointima formation. Circulation. 113:1879–1887. 2006. View Article : Google Scholar : PubMed/NCBI
7	Chaturvedi S, Bruno A, Feasby T, Holloway R, Benavente O, Cohen SN, Cote R, Hess D, Saver J, Spence JD, et al: Carotid endarterectomy-an evidence-based review: Report of the therepeutics and technology assessment subcommittee of the american academy of neurology. Neurology. 65:794–801. 2005. View Article : Google Scholar : PubMed/NCBI
8	Hay C, Micko C, Prescott MF, Liau G, Robinson K and De Leon H: Differential cell cycle progression patterns of infiltrating leukocytes and resident cells after balloon injury of the rat carotid artery. Arterioscler Thromb Vasc Biol. 21:1948–1954. 2001. View Article : Google Scholar : PubMed/NCBI
9	Li YT, Swales KE, Thomas GJ, Warner TD and Bishop-Bailey D: Farnesoid X receptor ligands inhibit vascular smooth muscle cell inflammation and migration. Arterioscler Thromb Vasc Biol. 27:2606–2611. 2007. View Article : Google Scholar : PubMed/NCBI
10	De Simone V, Franzè E, Ronchetti G, Colantoni A, Fantini MC, Di Fusco D, Sica GS, Sileri P, MacDonald TT, Pallone F, et al: Th17-type cytokines, IL-6 and TNF-α synergistically activate STAT3 and NF-κB to promote colorectal cancer cell growth. Oncogene. 34:3493–3503. 2015. View Article : Google Scholar : PubMed/NCBI
11	Couffinhal T, Duplàa C, Labat L, Lamaziere JM, Moreau C, Printseva O and Bonnet J: Tumor necrosis factor-alpha stimulates ICAM-1 expression in human vascular smooth muscle cells. Arterioscler Thromb. 13:407–414. 1993. View Article : Google Scholar : PubMed/NCBI
12	Selzman CH, Shames BD, Reznikov LL, Miller SA, Meng X, Barton HA, Werman A, Harken AH, Dinarello CA and Banerjee A: Liposomal delivery of purified inhibitory-kappBalpha inhibits tumor necrosis factor-alpha-induced human vascular smooth muscle proliferation. Circ Res. 84:867–875. 1999. View Article : Google Scholar : PubMed/NCBI
13	Nurmi A, Lindsberg PJ, Koistinaho M, Zhang W, Juettler E, Karjalainen-Lindsberg ML, Weih F, Frank N, Schwaninger M and Koistinaho J: Nuclear factor-kappaB contributes to infarction after permanent focal ischemia. Stroke. 35:987–991. 2004. View Article : Google Scholar : PubMed/NCBI
14	Williams AJ, Hale SL, Moffett JR, Dave JR, Elliott PJ, Adams J and Tortella FC: Delayed treatment with MLN519 reduces infarction and associated neurologic deficit caused by focal ischemic brain injury in rats via antiinflammatory mechanisms involving nuclear factor-kappaB activation, gliosis, and leukocyte infiltration. J Cereb Blood Flow Metab. 23:75–87. 2003. View Article : Google Scholar : PubMed/NCBI
15	Ross G, Jiang Y, Landberg G, Nielsen NH, Zhang P and Lee MY: Determination of the epitope of an inhibitory antibody to proliferating cell nuclear antigen. Exp Cell Res. 226:208–213. 1996. View Article : Google Scholar : PubMed/NCBI
16	Morikawa E, Zhang SM, Seko Y, Toyoda T and Kirino T: Treatment of focal cerebral ischemia with synthetic oligopeptide corresponding to lectin domain of selectin. Stroke. 27:951–955. 1996. View Article : Google Scholar : PubMed/NCBI
17	National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals: Guide for the Care and Use of Laboratory Animals. 8th. Washington (DC): National Academies Press (US); 2011
18	Chen G, Shi JX, Hang CH, Xie W, Liu J and Liu X: Inhibitory effect on cerebral inflammatory agents that accompany traumatic brain injury in a rat model: A potential neuroprotective mechanism of recombinant human erythropoietin (rhEPO). Neurosci Lett. 425:177–182. 2007. View Article : Google Scholar : PubMed/NCBI
19	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
20	Bassiouny HS, White S, Glagov S, Choi E, Giddens DP and Zarins CK: Anastomotic intimal hyperplasia: Mechanical injury or flow induced. J Vasc Surg. 15:708–716. 1992. View Article : Google Scholar : PubMed/NCBI
21	Austin GE, Ratliff NB, Hollman J, Tabei S and Phillips DF: Intimal proliferation of smooth muscle cells as an explanation for recurrent coronary artery stenosis after percutaneous transluminal coronary angioplasty. J Am Coll Cardiol. 6:369–375. 1985. View Article : Google Scholar : PubMed/NCBI
22	Hong YJ, Jeong MH, Song SJ, Sim DS, Kim JH, Lim KS, Hachinohe D, Ahmed K, Hwang SH, Lee MG, et al: Effects of ramiprilat-coated stents on neointimal hyperplasia, inflammation, and arterial healing in a porcine coronary restenosis model. Korean Circ J. 41:535–541. 2011. View Article : Google Scholar : PubMed/NCBI
23	Zhou XC, Huang RC, Zhang B, Yin D, Liang B, Wang SP, Guan QG, Sun XZ, Miao ZL, He XZ, et al: Inflammation inhibitory effects of sirolimus and paclitaxel-eluting stents on interleukin-1β-induced coronary artery in-stent restenosis in pigs. Chin Med J (Engl). 123:2405–2409. 2010.PubMed/NCBI
24	Warner SJ and Libby P: Human vascular smooth muscle cells. Target for and source of tumor necrosis factor. J Immunol. 142:100–109. 1989.PubMed/NCBI
25	Kranzhöfer R, Schmidt J, Pfeiffer CA, Hagl S, Libby P and Kübler W: Angiotensin induces inflammatory activation of human vascular smooth muscle cells. Arterioscler Thromb Vasc Biol. 19:1623–1629. 1999. View Article : Google Scholar : PubMed/NCBI
26	Sekiguchi K, Li X, Coker M, Flesch M, Barger PM, Sivasubramanian N and Mann DL: Cross-regulation between the renin-angiotensin system and inflammatory mediators in cardiac hypertrophy and failure. Cardiovasc Res. 63:433–442. 2004. View Article : Google Scholar : PubMed/NCBI
27	Hinz M, Krappmann D, Eichten A, Heder A, Scheidereit C and Strauss M: NF-kappaB function in growth control: Regulation of cyclin D1 expression and G0/G1-to-S-phase transition. Mol Cell Biol. 19:2690–2698. 1999. View Article : Google Scholar : PubMed/NCBI
28	Ramana KV, Chandra D, Srivastava S, Bhatnagar A, Aggarwal BB and Srivastava SK: Aldose reductase mediates mitogenic signaling in vascular smooth muscle cells. J Biol Chem. 277:32063–32070. 2002. View Article : Google Scholar : PubMed/NCBI
29	Shimizu N, Azuma N, Nishikawa T, Hirata S, Morishita R, Kaneda Y and Sasajima T: Effect on vein graft intimal hyperplasia of nuclear factor-kB decoy transfection using the second generation of HVJ vector. J Cardiovasc Surg (Torino). 48:463–470. 2007.PubMed/NCBI
30	Miyake T, Aoki M, Shiraya S, Tanemoto K, Ogihara T, Kaneda Y and Morishita R: Inhibitory effects of NFkappaB decoy oligodeoxynucleotides on neointimal hyperplasia in a rabbit vein graft model. J Mol Cell Cardiol. 41:431–440. 2006. View Article : Google Scholar : PubMed/NCBI
31	Gareus R, Kotsaki E, Xanthoulea S, van der Made I, Gijbels MJ, Kardakaris R, Polykratis A, Kollias G, de Winther MP and Pasparakis M: Endothelial cell-specific NF-kappaB inhibition protects mice from atherosclerosis. Cell Metab. 8:372–383. 2008. View Article : Google Scholar : PubMed/NCBI