Open Access

Paeoniflorin induces G2/M cell cycle arrest and caspase-dependent apoptosis through the upregulation of Bcl-2 X-associated protein and downregulation of B-cell lymphoma 2 in human osteosarcoma cells

  • Authors:
    • Li‑Bin Jin
    • Jian Zhu
    • Cheng‑Zhen Liang
    • Li‑Jiang Tao
    • Bing Liu
    • Wei Yu
    • Han Hui Zou
    • Jun‑Jie Wang
    • Huimin Tao
  • View Affiliations

  • Published online on: January 22, 2018     https://doi.org/10.3892/mmr.2018.8464
  • Pages: 5095-5101
  • Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Paeoniflorin (PF), extracted from the peony root, has been proved to possess antineoplastic activity in different cancer cell lines. However, it remains unclear whether PF has an antineoplastic effect against osteosarcoma cells. The present study investigated the effects and the specific mechanism of PF on various human osteosarcoma cell lines. Using the multiple methods to detect the activity of PF on HOS and Saos‑2 human osteosarcoma cell lines, including an MTS assay, flow cytometry, transmission electron microscopy and western blotting, it was demonstrated that PF induces inhibition of proliferation, G2/M phase cell cycle arrest and apoptosis in the osteosarcoma cell lines in vitro, and activation of cleaved‑caspase‑3 and cleaved‑poly (ADPribose) polymerase in a dose‑dependent manner. Furthermore, the pro‑apoptotic factors Bcl‑2 X‑associated protein and BH3 interacting domain death agonist were uregulated, while the anti‑apoptotic factors B‑cell lymphoma 2 (Bcl‑2) and Bcl‑2‑extra large were downregulated. In conclusion, these results demonstrated that PF has a promising therapeutic potential in for osteosarcoma.
View Figures
View References

Related Articles

Journal Cover

April-2018
Volume 17 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Jin LB, Zhu J, Liang CZ, Tao LJ, Liu B, Yu W, Zou HH, Wang JJ and Tao H: Paeoniflorin induces G2/M cell cycle arrest and caspase-dependent apoptosis through the upregulation of Bcl-2 X-associated protein and downregulation of B-cell lymphoma 2 in human osteosarcoma cells. Mol Med Rep 17: 5095-5101, 2018
APA
Jin, L., Zhu, J., Liang, C., Tao, L., Liu, B., Yu, W. ... Tao, H. (2018). Paeoniflorin induces G2/M cell cycle arrest and caspase-dependent apoptosis through the upregulation of Bcl-2 X-associated protein and downregulation of B-cell lymphoma 2 in human osteosarcoma cells. Molecular Medicine Reports, 17, 5095-5101. https://doi.org/10.3892/mmr.2018.8464
MLA
Jin, L., Zhu, J., Liang, C., Tao, L., Liu, B., Yu, W., Zou, H. H., Wang, J., Tao, H."Paeoniflorin induces G2/M cell cycle arrest and caspase-dependent apoptosis through the upregulation of Bcl-2 X-associated protein and downregulation of B-cell lymphoma 2 in human osteosarcoma cells". Molecular Medicine Reports 17.4 (2018): 5095-5101.
Chicago
Jin, L., Zhu, J., Liang, C., Tao, L., Liu, B., Yu, W., Zou, H. H., Wang, J., Tao, H."Paeoniflorin induces G2/M cell cycle arrest and caspase-dependent apoptosis through the upregulation of Bcl-2 X-associated protein and downregulation of B-cell lymphoma 2 in human osteosarcoma cells". Molecular Medicine Reports 17, no. 4 (2018): 5095-5101. https://doi.org/10.3892/mmr.2018.8464