Qiyusanlong decoction suppresses lung cancer in mice via Wnt/β-catenin pathway

Tong,Jia‑Bing; Zhang,Xing‑Xing; Wang,Xin‑Heng; Zeng,Shi‑Jie; Wang,Dan‑Yang; Zhang,Zhi‑Qiang; Hu,Jian; Yang,Chen; Li,Ze‑Geng

doi:10.3892/mmr.2018.8478

Qiyusanlong decoction suppresses lung cancer in mice via Wnt/β-catenin pathway

Authors:
- Jia‑Bing Tong
- Xing‑Xing Zhang
- Xin‑Heng Wang
- Shi‑Jie Zeng
- Dan‑Yang Wang
- Zhi‑Qiang Zhang
- Jian Hu
- Chen Yang
- Ze‑Geng Li
View Affiliations

Affiliations: Department of Respiratory, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui 230031, P.R. China, Key Laboratory of Xin An Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei, Anhui 230012, P.R. China
Published online on: January 24, 2018 https://doi.org/10.3892/mmr.2018.8478
Pages: 5320-5327

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Lung cancer is one of the most fatal cancers due to its high metastatic rate. Traditional Chinese medicine has been used in cancer patients for decades to improve quality of life and prolong survival time. The present study used a novel Qiyusanlong (QYSL) decoction composed of 10 kinds of Chinese medicine including astragalus membranaceus (Huangqi), polygonatumod oratum (yuzu), scolopendra (tianlong), pberetima (dilong), solanum nigrum (longkui), herbahedyotis (baihushecao), semen coicis (yiyiren), euphorbia helioscopia (zeqi), curcuma longa (eshu) and tendril-leaved fritillary bulb (chuanbei). The effects and function of the QYSL decoction remain to be elucidated. The present study established a mouse xenograft model using Lewis lung carcinoma cell injection and administered different doses of QYSL decoction to the mice. It was demonstrated that the chemotherapy drug Cisplatin (DDP) and QYSL decoction repressed lung tumor growth, and the inhibitory effect of DDP was more significant. Furthermore, QYSL decoction and DDP modulated the expression of regulatory proteins in the Wnt/β‑catenin pathway, including Wnt1, Wnt2, Wnt5a and glycogen synthase kinase 3β, detected by western blotting, and affected the signals of cluster of differentiation 44 variation 6 and Survivin in tumor tissues, examined via immunohistochemistry. The combination of QYSL decoction and DDP enhanced the inhibitory effect. These data demonstrated that the QYSL decoction repressed lung tumor development via the Wnt/β‑catenin pathway. The therapeutic effect of QYSL decoction alone was milder compared with DDP, however the combination of QYSL decoction and chemotherapy exhibited an increased the rapeutic effect compared with the treatments administered alone. These findings revealed the function of QYSL decoction as a lung cancer treatment and provided insight for a novel lung cancer therapy.

View Figures

View References

1	Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI
2	Hwang KE and Kim HR: Response evaluation of chemotherapy for lung cancer. Tuberc Respir Dis (Seoul). 80:136–142. 2017. View Article : Google Scholar : PubMed/NCBI
3	Yu N, Xiong Y and Wang C: Bu-Zhong-Yi-Qi decoction, the water extract of chinese traditional herbal medicine, enhances cisplatin cytotoxicity in A549/DDP cells through induction of apoptosis and autophagy. Biomed Res Int. 2017:36927972017. View Article : Google Scholar : PubMed/NCBI
4	Somasundaram A and Burns TF: The next generation of immunotherapy: Keeping lung cancer in check. J Hematol Oncol. 10:872017. View Article : Google Scholar : PubMed/NCBI
5	Ou Y, Zhai D, Wu N and Li X: Downregulation of miR-363 increases drug resistance in cisplatin-treated HepG2 by dysregulating Mcl-1. Gene. 572:116–122. 2015. View Article : Google Scholar : PubMed/NCBI
6	Lin HQ, Gong AG, Wang HY, Duan R, Dong TT, Zhao KJ and Tsim KW: Danggui Buxue Tang (Astragali Radix and Angelicae Sinensis Radix) for menopausal symptoms: A review. J Ethnopharmacol. 199:205–210. 2017. View Article : Google Scholar : PubMed/NCBI
7	Liu Z, Chen S, Cai J, Zhang E, Lan L, Zheng J, Liao L, Yang X, Zhou C and Du J: Traditional Chinese medicine syndrome-related herbal prescriptions in treatment of malignant tumors. J Tradit Chin Med. 33:19–26. 2013. View Article : Google Scholar : PubMed/NCBI
8	Zhang X, Tong J and Li Z: Qiyusanlong decoction inhibits the level of PD-1/PD-L1 in mice bearing Lewis lung carcinoma. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 32:770–774. 2016.(In Chinese). PubMed/NCBI
9	Jiang Q, He M, Guan S, Ma M, Wu H, Yu Z, Jiang L, Wang Y, Zong X, Jin F and Wei M: MicroRNA-100 suppresses the migration and invasion of breast cancer cells by targeting FZD-8 and inhibiting Wnt/β-catenin signaling pathway. Tumour Biol. 37:5001–5011. 2016. View Article : Google Scholar : PubMed/NCBI
10	Zhan P, Zhang B, Xi GM, Wu Y, Liu HB, Liu YF, Xu WJ, Zhu QQ, Cai F, Zhou ZJ, et al: PRC1 contributes to tumorigenesis of lung adenocarcinoma in association with the Wnt/β-catenin signaling pathway. Mol Cancer. 16:1082017. View Article : Google Scholar : PubMed/NCBI
11	Hu T and Li C: Convergence between Wnt-β-catenin and EGFR signaling in cancer. Mol Cancer. 9:2362010. View Article : Google Scholar : PubMed/NCBI
12	Shang S, Hua F and Hu ZW: The regulation of β-catenin activity and function in cancer: Therapeutic opportunities. Oncotarget. 8:33972–33989. 2017. View Article : Google Scholar : PubMed/NCBI
13	Salahshor S, Naidoo R, Serra S, Shih W, Tsao MS, Chetty R and Woodgett JR: Frequent accumulation of nuclear E-cadherin and alterations in the Wnt signaling pathway in esophageal squamous cell carcinomas. Mod Pathol. 21:271–281. 2008. View Article : Google Scholar : PubMed/NCBI
14	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
15	Ponta H, Sherman L and Herrlich PA: CD44: From adhesion molecules to signalling regulators. Nat Rev Mol Cell Biol. 4:33–45. 2003. View Article : Google Scholar : PubMed/NCBI
16	Wang S, Zhu L, Zuo W, Zeng Z, Huang L, Lin F, Lin R, Wang J, Lu J, Wang Q, et al: MicroRNA-mediated epigenetic targeting of survivin significantly enhances the antitumor activity of paclitaxel against non-small cell lung cancer. Oncotarget. 7:37693–37713. 2016.PubMed/NCBI
17	Zhuang B, Du L, Xu H, Xu X, Wang C, Fan Y, Cong M, Yin J, Li H and Guan H: Self-assembled Micelle Loading Cabazitaxel for therapy of Lung Cancer. Int J Pharm. 499:146–155. 2016. View Article : Google Scholar : PubMed/NCBI
18	Taixiang W, Munro AJ and Guanjian L: Chinese medical herbs for chemotherapy side effects in colorectal cancer patients. Cochrane Database Syst Rev: CD004540. 2005.
19	Xiao WL, Motley TJ, Unachukwu UJ, Lau CB, Jiang B, Hong F, Leung PC, Wang QF, Livingston PO, Cassileth BR and Kennelly EJ: Chemical and genetic assessment of variability in commercial Radix Astragali (Astragalus spp.) by ion trap LC-MS and nuclear ribosomal DNA barcoding sequence analyses. J Agric Food Chem. 59:1548–1556. 2011. View Article : Google Scholar : PubMed/NCBI
20	Tai Y, Sun YM, Zou X, Pan Q, Lan YD, Huo Q, Zhu JW, Guo F, Zheng CQ, Wu CZ and Liu H: Effect of Polygonatum odoratum extract on human breast cancer MDA-MB-231 cell proliferation and apoptosis. Exp Ther Med. 12:2681–2687. 2016. View Article : Google Scholar : PubMed/NCBI
21	Wu L, Liu T, Xiao Y, Li X, Zhu Y, Zhao Y, Bao J and Wu C: Polygonatum odoratum lectin induces apoptosis and autophagy by regulation of microRNA-1290 and microRNA-15a-3p in human lung adenocarcinoma A549 cells. Int J Biol Macromol. 85:217–226. 2016. View Article : Google Scholar : PubMed/NCBI
22	Ma W, Liu R, Qi J and Zhang Y: Extracts of centipede Scolopendra subspinipes mutilans induce cell cycle arrest and apoptosis in A375 human melanoma cells. Oncol Lett. 8:414–420. 2014. View Article : Google Scholar : PubMed/NCBI
23	Yang MY, Hung CH, Chang CH, Tseng TH and Wang CJ: Solanum nigrum suppress angiogenesis-mediated tumor growth through inhibition of the AKT/mTOR pathway. Am J Chin Med. 44:1273–1288. 2016. View Article : Google Scholar : PubMed/NCBI
24	Liu X, Xu F, Wang G, Diao X and Li Y: Kanglaite injection plus chemotherapy versus chemotherapy alone for non-small cell lung cancer patients: A systematic review and meta-analysis. Curr Ther Res Clin Exp. 69:381–411. 2008. View Article : Google Scholar : PubMed/NCBI
25	Cheng J, Han W, Wang Z, Shao Y, Wang Y and Zhang Y, Li Z, Xu X and Zhang Y: Hepatocellular carcinoma growth is inhibited by euphorbia helioscopia L. Extract in nude mice xenografts. Biomed Res Int. 2015:6010152015. View Article : Google Scholar : PubMed/NCBI
26	Kocaadam B and Şanlier N: Curcumin, an active component of turmeric (Curcuma longa), and its effects on health. Crit Rev Food Sci Nutr. 57:2889–2895. 2017. View Article : Google Scholar : PubMed/NCBI
27	MacDonald BT, Tamai K and He X: Wnt/beta-catenin signaling: Components, mechanisms, and diseases. Dev Cell. 17:9–26. 2009. View Article : Google Scholar : PubMed/NCBI
28	Zhao Y, Yang ZQ, Wang Y, Miao Y, Liu Y, Dai SD, Han Y and Wang EH: Dishevelled-1 and dishevelled-3 affect cell invasion mainly through canonical and noncanonical Wnt pathway, respectively and associate with poor prognosis in nonsmall cell lung cancer. Mol Carcinog. 49:760–770. 2010.PubMed/NCBI
29	Liu Y, Zhou Q, Zhou D, Huang C, Meng X and Li J: Secreted frizzled-related protein 2-mediated cancer events: Friend or foe? Pharmacol Rep. 69:403–408. 2017. View Article : Google Scholar : PubMed/NCBI
30	Wang J, Xiao L, Luo CH, Zhou H, Zeng L, Zhong J, Tang Y, Zhao XH, Zhao M and Zhang Y: CD44v6 promotes β-catenin and TGF-β expression, inducing aggression in ovarian cancer cells. Mol Med Rep. 11:3505–3510. 2015. View Article : Google Scholar : PubMed/NCBI
31	Sun W and Chen G: Impact and mechanism of non-steroidal anti-inflammatory drugs combined with chemotherapeutic drugs on human lung cancer-nude mouse transplanted tumors. Oncol Lett. 11:4193–4199. 2016. View Article : Google Scholar : PubMed/NCBI
32	Garg H, Suri P, Gupta JC, Talwar GP and Dubey S: Survivin: A unique target for tumor therapy. Cancer Cell Int. 16:492016. View Article : Google Scholar : PubMed/NCBI