Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box 1 protein

Zhang,Yunzhong; Feng,Jing; Cui,Jizhen; Yang,Guozheng; Zhu,Xianai

doi:10.3892/mmr.2018.8609

Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box 1 protein

Authors:
- Yunzhong Zhang
- Jing Feng
- Jizhen Cui
- Guozheng Yang
- Xianai Zhu
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Affiliations: Intensive Care Unit, Qingzhou Hospital, Taishan Medical University, Qingzhou, Shandong 262500, P.R. China, Department of Gynecology, Qingzhou Hospital, Taishan Medical University, Qingzhou, Shandong 262500, P.R. China
Published online on: February 15, 2018 https://doi.org/10.3892/mmr.2018.8609
Pages: 5805-5813
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study investigated the roles of pre-B cell leukemia transcription factor 3 (PBX3) in sepsis. Reverse transcription-quantitative polymerase chain reaction and western blot analysis indicated that overexpression of the PBX 3'‑untranslated region (UTR) promoted high mobility group box 1 (HMGB1) protein expression in human umbilical vein endothelial cells (HUVECs) (P<0.01). Furthermore, post‑treatment of PBX3 small interfering (si)RNA suppressed lipopolysaccharide (LPS)‑mediated HMGB1 release and attenuated HMGB1‑mediated hyperpermeability and leukocyte migration in HUVECs and septic mice (P<0.01). Additionally, post‑injection of PBX3 siRNA also induced the downregulation of cecal ligation and puncture‑induced HMGB1 release, production of IL‑6 and mortality (P<0.01). Mechanistically, the 3'UTRs of PBX3 and HMGB1 were identified to harbor six common micro (mi)RNA binding sites, and PBX 3'UTR increased HMGB1 expression in a 3'UTR‑ and miRNA‑dependent manner. Notably, the coding sequence of PBX3 did not increase HMGB1 expression in HUVECs. Collectively, the present study indicates that PBX 3'UTR may induce inflammatory responses and sepsis via acting as a competing endogenous RNA for HMGB1.

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