Open Access

Inhibition of mammalian target of rapamycin complex 1 signaling by n-3 polyunsaturated fatty acids promotes locomotor recovery after spinal cord injury

  • Authors:
    • Jiping Nie
    • Jian Chen
    • Jianguo Yang
    • Qinqin Pei
    • Jing Li
    • Jia Liu
    • Lixin Xu
    • Nan Li
    • Youhao Chen
    • Xiaohua Chen
    • Hao Luo
    • Tiansheng Sun
  • View Affiliations

  • Published online on: February 8, 2018     https://doi.org/10.3892/mmr.2018.8583
  • Pages: 5894-5902
  • Copyright: © Nie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to explore the effects of n‑3 polyunsaturated fatty acids (PUFAs) on autophagy and their potential for promoting locomotor recovery after spinal cord injury (SCI). Primary neurons were isolated and cultured. Sprague‑Dawley rats were randomly divided into three groups and fed diets with different amounts of n‑3 PUFAs. A model of spinal cord contusion was created at the T10 spinal segment and the composition of PUFAs was analyzed using gas chromatography. Spinal repair and motor function were evaluated postoperatively. Assessment of the effects of n‑3 PUFAs on autophagy and mammalian target of rapamycin complex 1 (mTORC1) was performed using immunofluorescence staining and western blotting. In vitro, n‑3 PUFAs inhibited mTORC1 and enhanced autophagy. The n‑3 PUFA levels and the ratio of n‑3 PUFA to n‑6 PUFA in the spinal cord and serum of rats fed a high‑n‑3 PUFA diet were higher before and after operation (P<0.05). Additionally, rats in the high‑n‑3 PUFA group showed improved motor function recovery, spinal cord repair‑related protein expression level (MBP, Galc and GFAP). Expression levels if these protiens in the high‑n‑3 PUFA diet group expressed the highest levels, followed by the low‑n‑3 PUFA diet group and finally the control group (P<0.05). high‑n‑3 PUFA diet promoted autophagy ability and inhibited activity of the mTORC1 signaling pathway compared with the low‑n‑3 PUFA diet group or the control group (P<0.05). These results suggest that exogenous dietary n‑3 PUFAs can inhibit mTORC1 signaling and enhance autophagy, promoting functional recovery of rats with SCI.
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April-2018
Volume 17 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Nie J, Chen J, Yang J, Pei Q, Li J, Liu J, Xu L, Li N, Chen Y, Chen X, Chen X, et al: Inhibition of mammalian target of rapamycin complex 1 signaling by n-3 polyunsaturated fatty acids promotes locomotor recovery after spinal cord injury. Mol Med Rep 17: 5894-5902, 2018
APA
Nie, J., Chen, J., Yang, J., Pei, Q., Li, J., Liu, J. ... Sun, T. (2018). Inhibition of mammalian target of rapamycin complex 1 signaling by n-3 polyunsaturated fatty acids promotes locomotor recovery after spinal cord injury. Molecular Medicine Reports, 17, 5894-5902. https://doi.org/10.3892/mmr.2018.8583
MLA
Nie, J., Chen, J., Yang, J., Pei, Q., Li, J., Liu, J., Xu, L., Li, N., Chen, Y., Chen, X., Luo, H., Sun, T."Inhibition of mammalian target of rapamycin complex 1 signaling by n-3 polyunsaturated fatty acids promotes locomotor recovery after spinal cord injury". Molecular Medicine Reports 17.4 (2018): 5894-5902.
Chicago
Nie, J., Chen, J., Yang, J., Pei, Q., Li, J., Liu, J., Xu, L., Li, N., Chen, Y., Chen, X., Luo, H., Sun, T."Inhibition of mammalian target of rapamycin complex 1 signaling by n-3 polyunsaturated fatty acids promotes locomotor recovery after spinal cord injury". Molecular Medicine Reports 17, no. 4 (2018): 5894-5902. https://doi.org/10.3892/mmr.2018.8583