The therapeutic effect of artesunate on rosacea through the inhibition of the JAK/STAT signaling pathway Retraction in /10.3892/mmr.2023.13127

Li,Ting; Zeng,Qingwen; Chen,Xingming; Wang,Guojiang; Zhang,Haiqing; Yu,Aihua; Wang,Hairui; Hu,Yang

doi:10.3892/mmr.2018.8887

The therapeutic effect of artesunate on rosacea through the inhibition of the JAK/STAT signaling pathway

Retraction in: /10.3892/mmr.2023.13127

Authors:
- Ting Li
- Qingwen Zeng
- Xingming Chen
- Guojiang Wang
- Haiqing Zhang
- Aihua Yu
- Hairui Wang
- Yang Hu
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Affiliations: Department of Dermatology, Nanxiang Hospital of Jiading, Shanghai 201802, P.R. China, Department of Pediatric Endocrinology/Genetics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai Institute for Pediatric Research, Shanghai 200092, P.R. China, Department of Dermatology, Zhoupu Hospital of Pudong, Shanghai 201318, P.R. China
Published online on: April 16, 2018 https://doi.org/10.3892/mmr.2018.8887
Pages: 8385-8390

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Abstract

Acne rosacea is a type of chronic dermatosis with the characteristics of erubescence, angiotelectasis and pustule formation. However, current treatment methods are limited due to the side effects. Artesunate demonstrated a promising therapeutic efficacy with a high safety margin. HaCaT cells were treated with antibacterial peptide LL‑37 to simulate rosacea caused by Demodex folliculorum (D. folliculorum) infection. Cell Counting kit 8 and flow cytometry assays were performed to measure cellular proliferation, apoptosis, the stage of the cell cycle and reactive oxygen species generation in order to determine the level of cell damage. Then the damaged cells were treated with different concentrations of artesunate and doxycycline to determine the therapeutic effect of artesunate. Pro‑inflammatory cytokines tumor necrosis factor‑α (TNF‑α), interleukin (IL)‑6, IL‑8 and C‑C motif chemokine 2 (MCP‑1) were measured using an ELISA, while western blotting was used to detect the expression of Janus kinase 2 (JAK2) and signal transducer and transcription activator (STAT3). As a result, LL‑37 treated HaCaT cells decreased in cell viability, had an increased apoptotic rate and cell cycle arrest, indicating that cell damage caused by rosacea was simulated. In addition, upregulated concentrations of the pro‑inflammatory cytokines TNF‑α, IL‑6, IL‑8 and MCP‑1 were attenuated in the artesunate group in a dose‑dependent fashion, indicating the therapeutic effect of artesunate. Furthermore, higher concentrations of artesunate exhibited an improved effect compared with the doxycycline group. In addition, increased expression levels of JAK2 and STAT3 following treatment with LL‑37 suggested that rosacea caused by D. folliculorum infection may lead to inflammation through the JAK/STAT signaling pathway. In conclusion, the potential mechanism by which damage occurs in rosacea was revealed and a promising therapeutic method against rosacea was demonstrated.

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