Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
August-2018 Volume 18 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2018 Volume 18 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Recombinant club cell protein 16 (CC16) ameliorates cigarette smoke‑induced lung inflammation in a murine disease model of COPD

  • Authors:
    • Min Pang
    • Hong‑Yan Liu
    • Ting Li
    • Dan Wang
    • Xiao‑Yun Hu
    • Xin‑Ri Zhang
    • Bao‑Feng Yu
    • Rui Guo
    • Hai‑Long Wang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, The First Hospital, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China, School of Basic Medicine; Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China
    Copyright: © Pang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2198-2206
    |
    Published online on: June 25, 2018
       https://doi.org/10.3892/mmr.2018.9216
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Club cell protein (CC16) is expressed primarily by club cells possesses anti‑inflammatory properties and is located in the bronchiolar epithelium. Previous studies have demonstrated that CC16 deficiency is associated with the progression of chronic obstructive pulmonary disease (COPD). In the present study, the therapeutic effects of recombinant rat CC16 protein in mice with COPD were examined and the underlying mechanisms investigated. A total of 30 adult male C57/BL6 mice were randomly divided into three groups (10 mice/group). A mouse COPD model was generated by exposing 20 mice to cigarette smoke (CS) for 24 weeks. A total of 10 mice were treated intranasally with rCC16 (2.5 µg/g body weight) and control mice were exposed to normal room air. Results indicated that rCC16 treatment ameliorated pathological damage in the lungs and reduced the production of tumor necrosis factor (TNF)‑α, interleukin (IL)‑6 and IL‑8, which were induced by CS exposure. After rCC16 administration, endogenous CC16 was upregulated and the body weight of COPD mice was increased, whereas the opposite was observed in CS‑exposed mice. Additionally, rCC16 treatment inhibited the DNA binding of NF‑κB/p65 in lung tissues and reduced nuclear translocation of NF‑κB/p65 in BALF and epithelial cells. Moreover, rCC16 treatment lead to a decrease in the total number of BALF cells, including macrophages, which was elevated in COPD mice. In conclusion, the present results demonstrate that rCC16 has therapeutic effects on COPD by downregulating pro‑inflammatory factors via the NF‑κB pathway.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Khan MA, Kianpour S, Stämpfli MR and Janssen LJ: Kinetics of in vitro bronchoconstriction in an elastolytic mouse model of emphysema. Eur Respir J. 30:691–700. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Van Dijk EM, Culha S, Menzen MH, Bidan CM and Gosens R: Elastase-induced parenchymal disruption and airway hyper responsiveness in mouse precision cut lung slices: Toward an ex vivo COPD model. Front Physiol. 7:6572016.PubMed/NCBI

3 

Rabe KF, Hurd S, Anzueto A, Barnes PJ, Buist SA, Calverley P, Fukuchi Y, Jenkins C, Rodriguez-Roisin R, van Weel C, et al: Global strategy for the diagnosis, management and prevention of chronic obstructive pulmonary disease: GOLD executive summary. Am J Respir Crit Care Med. 176:532–555. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Hogg JC, Chu F, Utokaparch S, Woods R, Elliott WM, Buzatu L, Cherniack RM, Rogers RM, Sciurba FC, Coxson HO and Paré PD: The nature of small-airway obstruction in chronic obstructive pulmonary disease. N Engl J Med. 350:2645–2653. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Barnes PJ: Alveolar macrophages as orchestrators of COPD. Copd. 1:59–70. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Barnes PJ: Inflammatory mechanisms in patients with chronic obstructive pulmonary disease. J Allergy Clin Immunol. 138:16–27. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Di Marco F, Santus P, Scichilone N, Solidoro P, Contoli M, Braido F and Corsico AG: Symptom variability and control in COPD: Advantages of dual bronchodilation therapy. Respir Med. 125:49–56. 2017. View Article : Google Scholar : PubMed/NCBI

8 

Barnes PJ: Corticosteroid resistance in patients with asthma and chronic obstructive pulmonary disease. J Allergy Clin Immunol. 131:636–645. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Lee JH, Koo TH, Yoon H, Jung HS, Jin HZ, Lee K, Hong YS and Lee JJ: Inhibition of NF-kappa B activation through targeting I kappa B kinase by celastrol, a quinone methide triterpenoid. Biochem Pharmacol. 72:1311–1321. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Park HJ, Kim IT, Won JH, Jeong SH, Park EY, Nam JH, Choi J and Lee KT: Anti-inflammatory activities of ent-16alphaH,17-hydroxy-kauran-19-oic acid isolated from the roots of Siegesbeckia pubescens are due to the inhibition of iNOS and COX-2 expression in RAW 264.7 macrophages via NF-kappaB inactivation. Eur J Pharmacol. 558:185–193. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Caramori G, Romagnoli M, Casolari P, Bellettato C, Casoni G, Boschetto P, Chung KF, Barnes PJ, Adcock IM, Ciaccia A, et al: Nuclear localisation of p65 in sputum macrophages but not in sputum neutrophils during COPD exacerbations. Thorax. 58:348–351. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Di Stefano A, Caramori G, Oates T, Capelli A, Lusuardi M, Gnemmi I, Ioli F, Chung KF, Donner CF, Barnes PJ and Adcock IM: Increased expression of nuclear factor-kappaB in bronchial biopsies from smokers and patients with COPD. Eur Respir J. 20:556–563. 2002. View Article : Google Scholar : PubMed/NCBI

13 

Zhuan B, Yu Y, Yang Z, Zhao X and Li P: Mechanisms of oxidative stress effects of the NADPH oxidase-ROS-NF-κB transduction pathway and VPO1 on patients with chronic obstructive pulmonary disease combined with pulmonary hypertension. Eur Rev Med Pharmacol Sci. 21:3459–3464. 2017.PubMed/NCBI

14 

Edwards MR, Bartlett NW, Clarke D, Birrell M, Belvisi M and Johnston SL: Targeting the NF-kappaB pathway in asthma and chronic obstructive pulmonary disease. Pharmacol Ther. 121:1–13. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Nogaki T, Asano K, Furuta A, Kanai K, Suzaki I, Kanei A and Suzaki H: Enhancement of clara cell 10-kD protein (CC10) production from nasal epithelial cells by fexofenadine hydrochloride. Asian Pac J Allergy Immunol. 30:139–145. 2012.PubMed/NCBI

16 

Mukherjee AB, Kundu GC, Mantile-Selvaggi G, Yuan CJ, Mandal AK, Chattopadhyay S, Zheng F, Pattabiraman N and Zhang Z: Uteroglobin: A novel cytokine? Cell Mol Life Sci. 55:771–787. 1999. View Article : Google Scholar : PubMed/NCBI

17 

Johansson S, Keen C, Ståhl A, Wennergren G and Benson M: Low levels of CC16 in nasal fluid of children with birch pollen-induced rhinitis. Allergy. 60:638–642. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Wang H, Long XB, Cao PP, Wang N, Liu Y, Cui YH, Huang SK and Liu Z: Clara cell 10-kD protein suppresses chitinase 3-like 1 expression associated with eosinophilic chronic rhinosinusitis. Am J Respir Crit Care Med. 181:908–916. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Long XB, Hu S, Wang N, Zhen HT, Cui YH and Liu Z: Clara cell 10-kDa protein gene transfection inhibits NF-κB activity in airway epithelial cells. PLoS one. 7:e359602012. View Article : Google Scholar : PubMed/NCBI

20 

Van Vyve T, Chanez P, Bernard A, Bousquet J, Godard P, Lauwerijs R and Sibille Y: Protein content in bronchoalveolar lavage fluid of patients with asthma and control subjects. J Allergy Clin Immunol. 95:60–68. 1995. View Article : Google Scholar : PubMed/NCBI

21 

Pang M, Wang H, Bai JZ, Cao D, Jiang Y, Zhang C, Liu Z, Zhang X, Hu X, Xu J and Du Y: Recombinant rat CC16 protein inhibits LPS-induced MMP-9 expression via NF-kappaB pathway in rat tracheal epithelial cells. Exp Biol Med. 240:1266–1278. 2015. View Article : Google Scholar

22 

Pang M, Yuan Y, Wang D, Li T, Wang D, Shi X, Guo M, Wang C, Zhang X, Zheng G, et al: Recombinant CC16 protein inhibits the production of pro-inflammatory cytokines via NF-kappaB and p38 MAPK pathways in LPS-activated RAW264.7 macrophages. Acta biochimica et biophysica Sinica. 1–9. 2017.

23 

Bayne K: Revised Guide for the Care and Use of Laboratory Animals available. American Physiological Society. Physiologist. 39(199): 208–111. 1996.

24 

Ma R, Gong X, Jiang H, Lin C, Chen Y, Xu X, Zhang C, Wang J, Lu W and Zhong N: Reduced nuclear translocation of serum response factor is associated with skeletal muscle atrophy in a cigarette smoke-induced mouse model of COPD. Int J Chron Obstruct Pulmon Dis. 12:581–587. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Wolfson MR, Funanage VL, Kirwin SM, Pilon AL, Shashikant BN, Miller TL and Shaffer TH: Recombinant human Clara cell secretory protein treatment increases lung mRNA expression of surfactant proteins and vascular endothelial growth factor in a premature lamb model of respiratory distress syndrome. Am J Perinatol. 25:637–645. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Levine CR, Gewolb IH, Allen K, Welch RW, Melby JM, Pollack S, Shaffer T, Pilon AL and Davis JM: The safety, pharmacokinetics and anti-inflammatory effects of intratracheal recombinant human Clara cell protein in premature infants with respiratory distress syndrome. Pediatr Res. 58:15–21. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Chilkoti A, Tan PH and Stayton PS: Site-directed mutagenesis studies of the high-affinity streptavidin-biotin complex: Contributions of tryptophan residues 79, 108 and 120. Proc Natl Acad Sci USA. 92:1754–1758. 1995. View Article : Google Scholar : PubMed/NCBI

28 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

29 

Yan C, Wang H, Aggarwal B and Boyd DD: A novel homologous recombination system to study 92 kDa type IV collagenase transcription demonstrates that the NF-kappaB motif drives the transition from a repressed to an activated state of gene expression. FASEB J. 18:540–541. 2004. View Article : Google Scholar : PubMed/NCBI

30 

Laucho-Contreras ME, Polverino F, Tesfaigzi Y, Pilon A, Celli BR and Owen CA: Club cell protein 16 (CC16) augmentation: A potential disease-modifying approach for chronic obstructive pulmonary disease (COPD). Expert Opin Ther Targets. 20:869–883. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Nicholas A, Jeon H, Selasi GN, Na SH, Kwon HI, Kim YJ, Choi CW, Kim SI and Lee JC: Clostridium difficile-derived membrane vesicles induce the expression of pro-inflammatory cytokine genes and cytotoxicity in colonic epithelial cells in vitro. Microb Pathog. 107:6–11. 2017. View Article : Google Scholar : PubMed/NCBI

32 

Nishino R, Fukuyama T, Watanabe Y, Kurosawa Y, Kosaka T and Harada T: Significant upregulation of cytokine secretion from T helper type 9 and 17 cells in a NC/Nga mouse model of ambient chemical exposure-induced respiratory allergy. J Pharmacol Toxicol Methods. 80:35–42. 2016. View Article : Google Scholar : PubMed/NCBI

33 

Shen LL, Liu YN, Shen HJ, Wen C, Jia YL, Dong XW, Jin F, Chen XP, Sun Y and Xie QM: Inhalation of glycopyrronium inhibits cigarette smoke-induced acute lung inflammation in a murine model of COPD. Int Immunopharmacol. 18:358–364. 2014. View Article : Google Scholar : PubMed/NCBI

34 

Irander K, Palm JP, Borres MP and Ghafouri B: Clara cell protein in nasal lavage fluid and nasal nitric oxide-biomarkers with anti-inflammatory properties in allergic rhinitis. Clin Mol Allergy. 10:42012. View Article : Google Scholar : PubMed/NCBI

35 

Bernard A, Marchandise FX, Depelchin S, Lauwerys R and Sibille Y: Clara cell protein in serum and bronchoalveolar lavage. Eur Respir J. 5:1231–1238. 1992.PubMed/NCBI

36 

Lomas DA, Silverman EK, Edwards LD, Locantore NW, Miller BE, Horstman DH and Tal-Singer R: Evaluation of COPD Longitudinally to Identify Predictive Surrogate Endpoints study investigators: Serum surfactant protein D is steroid sensitive and associated with exacerbations of COPD. Eur Respir J. 34:95–102. 2009. View Article : Google Scholar : PubMed/NCBI

37 

Pilette C, Godding V, Kiss R, Delos M, Verbeken E, Decaestecker C, De Paepe K, Vaerman JP, Decramer M and Sibille Y: Reduced epithelial expression of secretory component in small airways correlates with airflow obstruction in chronic obstructive pulmonary disease. Am J Respir Crit Care Med. 163:185–194. 2001. View Article : Google Scholar : PubMed/NCBI

38 

Laucho-Contreras ME, Polverino F, Gupta K, Taylor KL, Kelly E, Pinto-Plata V, Divo M, Ashfaq N, Petersen H, Stripp B, et al: Protective role for club cell secretory protein-16 (CC16) in the development of COPD. Eur Respir J. 45:1544–1556. 2015. View Article : Google Scholar : PubMed/NCBI

39 

Chen J, Lam S, Pilon A, McWilliams A, Macaulay C and Szabo E: Higher levels of the anti-inflammatory protein CC10 are associated with improvement in bronchial dysplasia and sputum cytometric assessment in individuals at high risk for lung cancer. Clin Cancer Res. 14:1590–1597. 2008. View Article : Google Scholar : PubMed/NCBI

40 

Zhu L, Di PY, Wu R, Pinkerton KE and Chen Y: Repression of CC16 by cigarette smoke (CS) exposure. PLoS One. 10:e01161592015. View Article : Google Scholar : PubMed/NCBI

41 

Miller TL, Shashikant BN, Melby JM, Pilon AL, Shaffer TH and Wolfson MR: Recombinant human Clara cell secretory protein in acute lung injury of the rabbit: effect of route of administration. Pediatr Crit Care Med. 6:698–706. 2005. View Article : Google Scholar : PubMed/NCBI

42 

Ma H, Wang H, Luo Y, Guo S and Song C: Mir-20b-induced increase in myeloid-derived suppressor cells in the lungs of mice with chronic asthma. Ann Clin Lab Sci. 47:76–82. 2017.PubMed/NCBI

43 

Reno FE, Normand P, McInally K, Silo S, Stotland P, Triest M, Carballo D and Piché C: A novel nasal powder formulation of glucagon: Toxicology studies in animal models. BMC Pharmacol Toxicol. 16:292015. View Article : Google Scholar : PubMed/NCBI

44 

Drost EM and MacNee W: Potential role of IL-8, platelet-activating factor and TNF-alpha in the sequestration of neutrophils in the lung: Effects on neutrophil deformability, adhesion receptor expression and chemotaxis. Eur J Immunol. 32:393–403. 2002. View Article : Google Scholar : PubMed/NCBI

45 

Gibson PG, Simpson JL and Saltos N: Heterogeneity of airway inflammation in persistent asthma: Evidence of neutrophilic inflammation and increased sputum interleukin-8. Chest. 119:1329–1336. 2001. View Article : Google Scholar : PubMed/NCBI

46 

Kaku Y, Imaoka H, Morimatsu Y, Komohara Y, Ohnishi K, Oda H, Takenaka S, Matsuoka M, Kawayama T, Takeya M, et al: Overexpression of CD163, CD204 and CD206 on alveolar macrophages in the lungs of patients with severe chronic obstructive pulmonary disease. PLoS One. 9:e874002014. View Article : Google Scholar : PubMed/NCBI

47 

Shapiro SD: The macrophage in chronic obstructive pulmonary disease. Am J Respir Crit Care Med. 160:S29–S32. 1999. View Article : Google Scholar : PubMed/NCBI

48 

Oeckinghaus A, Hayden MS and Ghosh S: Crosstalk in NF-κB signaling pathways. Nat Immunol. 12:695–708. 2011. View Article : Google Scholar : PubMed/NCBI

49 

Zhao L, Song Y, Pu J, Guo J, Wang Y, Chen Z, Chen T, Gu Y and Jia G: Effects of repeated Cr (VI) intratracheal instillation on club (Clara) cells and activation of nuclear factor-kappa B pathway via oxidative stress. Toxicol Lett. 231:72–81. 2014. View Article : Google Scholar : PubMed/NCBI

50 

Song W, Liu G, Bosworth CA, Walker JR, Megaw GA, Lazrak A, Abraham E, Sullender WM and Matalon S: Respiratory syncytial virus inhibits lung epithelial Na+ channels by up-regulating inducible nitric-oxide synthase. J Biol Chem. 284:7294–7306. 2009. View Article : Google Scholar : PubMed/NCBI

51 

Baeuerle PA and Henkel T: Function and activation of NF-kappa B in the immune system. Annu Rev Immunol. 12:141–179. 1994. View Article : Google Scholar : PubMed/NCBI

52 

Sahebjami H and Sathianpitayakul E: Influence of body weight on the severity of dyspnea in chronic obstructive pulmonary disease. Am J Respir Crit Care Med. 161:886–890. 2000. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Pang M, Liu HY, Li T, Wang D, Hu XY, Zhang XR, Yu BF, Guo R and Wang HL: Recombinant club cell protein 16 (CC16) ameliorates cigarette smoke‑induced lung inflammation in a murine disease model of COPD. Mol Med Rep 18: 2198-2206, 2018.
APA
Pang, M., Liu, H., Li, T., Wang, D., Hu, X., Zhang, X. ... Wang, H. (2018). Recombinant club cell protein 16 (CC16) ameliorates cigarette smoke‑induced lung inflammation in a murine disease model of COPD. Molecular Medicine Reports, 18, 2198-2206. https://doi.org/10.3892/mmr.2018.9216
MLA
Pang, M., Liu, H., Li, T., Wang, D., Hu, X., Zhang, X., Yu, B., Guo, R., Wang, H."Recombinant club cell protein 16 (CC16) ameliorates cigarette smoke‑induced lung inflammation in a murine disease model of COPD". Molecular Medicine Reports 18.2 (2018): 2198-2206.
Chicago
Pang, M., Liu, H., Li, T., Wang, D., Hu, X., Zhang, X., Yu, B., Guo, R., Wang, H."Recombinant club cell protein 16 (CC16) ameliorates cigarette smoke‑induced lung inflammation in a murine disease model of COPD". Molecular Medicine Reports 18, no. 2 (2018): 2198-2206. https://doi.org/10.3892/mmr.2018.9216
Copy and paste a formatted citation
x
Spandidos Publications style
Pang M, Liu HY, Li T, Wang D, Hu XY, Zhang XR, Yu BF, Guo R and Wang HL: Recombinant club cell protein 16 (CC16) ameliorates cigarette smoke‑induced lung inflammation in a murine disease model of COPD. Mol Med Rep 18: 2198-2206, 2018.
APA
Pang, M., Liu, H., Li, T., Wang, D., Hu, X., Zhang, X. ... Wang, H. (2018). Recombinant club cell protein 16 (CC16) ameliorates cigarette smoke‑induced lung inflammation in a murine disease model of COPD. Molecular Medicine Reports, 18, 2198-2206. https://doi.org/10.3892/mmr.2018.9216
MLA
Pang, M., Liu, H., Li, T., Wang, D., Hu, X., Zhang, X., Yu, B., Guo, R., Wang, H."Recombinant club cell protein 16 (CC16) ameliorates cigarette smoke‑induced lung inflammation in a murine disease model of COPD". Molecular Medicine Reports 18.2 (2018): 2198-2206.
Chicago
Pang, M., Liu, H., Li, T., Wang, D., Hu, X., Zhang, X., Yu, B., Guo, R., Wang, H."Recombinant club cell protein 16 (CC16) ameliorates cigarette smoke‑induced lung inflammation in a murine disease model of COPD". Molecular Medicine Reports 18, no. 2 (2018): 2198-2206. https://doi.org/10.3892/mmr.2018.9216
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team