Attenuated endothelial function is associated with decreased endothelial progenitor cells and nitric oxide in premenopausal diabetic women

Liu,Juan; Hu,Da‑Jun; Yan,Huiyang; Liu,Jianbin; Ai,Xixong; Ren,Zi; Zeng,Haitao; He,Hao; Yang,Zhen

doi:10.3892/mmr.2018.9451

Attenuated endothelial function is associated with decreased endothelial progenitor cells and nitric oxide in premenopausal diabetic women

Authors:
- Juan Liu
- Da‑Jun Hu
- Huiyang Yan
- Jianbin Liu
- Xixong Ai
- Zi Ren
- Haitao Zeng
- Hao He
- Zhen Yang
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Affiliations: Department of Endocrinology, The First Affiliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China, Department of Cardiology, The First Hospital of Chenzhou, Chenzhou, Hunan 423000, P.R. China, Guangzhou Beijing Community Health Service Center, Guangzhou, Guangdong 510080, P.R. China, Centre for Eye Research Australia, Faculty of Medicine, Dentistry and Health Science, The University of Melbourne, East Melbourne, VIC 3002, Australia, Center for Reproductive Medicine, The Sixth Affiliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China, Department of Cardiology, The Fifth Affiliated Hospital of Zunyi Medical University, Zhuhai, Guangdong 519100, P.R. China, Division of Emergency Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510080, P.R. China
Published online on: September 4, 2018 https://doi.org/10.3892/mmr.2018.9451
Pages: 4666-4674

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Abstract

Previous studies have demonstrated that the deleterious effect of diabetes mellitus (DM) on the risk of cardiovascular disease also occurs in premenopausal women, in spite of their relatively high estrogen levels; however, the underlying mechanism remains unclear. The present study aimed to investigate the sex‑related differences in circulating endothelial progenitor cells (EPCs) in a relatively young population with type 2 DM (T2DM) and its underlying mechanism. The number and functional activity of circulating EPCs, and vascular endothelial function assessed using flow‑mediated dilation (FMD), were compared in premenopausal women and age‑matched men with or without T2DM. Nitric oxide (NO) in the plasma or NO secreted by EPCs was also measured. The number and activity of circulating EPCs, and NO levels in the plasma or culture medium, were lower in premenopausal women with T2DM compared with those without T2DM. In addition, the number and activity of circulating EPCs and NO levels were decreased in men with T2DM compared with in age‑matched premenopausal women with T2DM. FMD was positively correlated with the number and activity of circulating EPCs, and NO levels. In conclusion, DM in premenopausal women may significantly impair the repair capability of EPCs and lead to endothelial dysfunction, which may be associated with reduced NO production. In patients with both DM and normal glucose tolerance, sex‑related differences of EPCs are presented in a young population.

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