Enhanced adhesion and proliferation of bone marrow mesenchymal stem cells on β‑tricalcium phosphate modified by an affinity peptide

Wang,Guozong; Man,Zhentao; Xin,Hua; Li,Yi; Wu,Changshun; Sun,Shui

doi:10.3892/mmr.2018.9655

Enhanced adhesion and proliferation of bone marrow mesenchymal stem cells on β‑tricalcium phosphate modified by an affinity peptide

Authors:
- Guozong Wang
- Zhentao Man
- Hua Xin
- Yi Li
- Changshun Wu
- Shui Sun
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Affiliations: Department of Joint Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Department of Neurology, People's Hospital of Rizhao, Rizhao, Shandong 222000, P.R. China
Published online on: November 13, 2018 https://doi.org/10.3892/mmr.2018.9655
Pages: 375-381
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Mesenchymal stem cells (MSCs) are often used in orthopedic tissue engineering, and bone marrow‑derived mesenchymal stem cells (BMSCs) are currently considered the gold standard. One of the most important issues in MSC‑based tissue engineering therapy is the low number of MSCs in pathological tissues. Achieving efficient recruitment of MSCs to defective or damaged tissues in vivo has been a difficult hurdle. The aim of the present study was to construct a biomaterial that can effectively recruit BMSCs to facilitate the repair of pathological tissues. So functional β‑tricalcium phosphate (β‑TCP) was synthesized using the BMSC affinity peptide DPIYALSWSGMA (DPI) adsorbed onto β‑TCP through an adsorption/freeze‑drying strategy. C57BL/6 mouse‑derived BMSCs were seeded onto the DPI peptide‑modified β‑TCP (β‑TCP‑DPI); in vitro experiments demonstrated that β‑TCP‑DPI enhanced BMSC adhesion and proliferation compared with unmodified β‑TCP. Results from the present study indicated that functional β‑TCP may be used as an ideal scaffold in tissue engineering and in regenerative medicine.

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