Involvement of endothelial nitric oxide synthase pathway in IGF‑1 protects endothelial progenitor cells against injury from oxidized LDLs

  • Authors:
    • Hao Jing‑Wen
    • Guo‑Feng Liu
    • Li‑Zhi Xiao
    • Yong‑Gang Wu
  • View Affiliations

  • Published online on: November 8, 2018     https://doi.org/10.3892/mmr.2018.9633
  • Pages: 660-666
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Abstract

A high level of oxidized low‑density lipoproteins (oxLDLs) is an independent risk factor for cardiovascular disease. The aim of the present study was to investigate whether insulin‑like growth factor‑1 (IGF‑1) protected endothelial progenitor cells (EPCs) from injury caused by ox‑LDLs, and whether the endothelial nitric oxide synthase (eNOS)/nitric oxide (NO) pathway was involved in this process. EPCs were isolated from human peripheral blood and characterized. In order to evaluate the effect of IGF‑1 on EPCs, cells were incubated with ox‑LDLs (100 mg/ml) for 24 h to induce a model of EPC dysfunction in vitro, which demonstrated a decrease in the number of EPCs, concomitant with increased apoptosis and decreased proliferation rates. IGF‑1 dose‑dependently increased the number of EPCs. Concurrently, IGF‑1 decreased the levels of apoptosis of EPCs and improved EPCs proliferation following ox‑LDLs challenge. In addition, IGF‑1 significantly increased NO levels in ox‑LDLs‑treated EPCs, accompanied by an upregulation in eNOS expression. The protective effects of IGF‑1 on EPCs and NO production were abolished by L‑NAME, a specific eNOS inhibitor. These results suggested that IGF‑1 protects EPCs from dysfunction induced by oxLDLs through a mechanism involving the eNOS/NO pathway.
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January-2019
Volume 19 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Jing‑Wen H, Liu GF, Xiao LZ and Wu YG: Involvement of endothelial nitric oxide synthase pathway in IGF‑1 protects endothelial progenitor cells against injury from oxidized LDLs. Mol Med Rep 19: 660-666, 2019
APA
Jing‑Wen, H., Liu, G., Xiao, L., & Wu, Y. (2019). Involvement of endothelial nitric oxide synthase pathway in IGF‑1 protects endothelial progenitor cells against injury from oxidized LDLs. Molecular Medicine Reports, 19, 660-666. https://doi.org/10.3892/mmr.2018.9633
MLA
Jing‑Wen, H., Liu, G., Xiao, L., Wu, Y."Involvement of endothelial nitric oxide synthase pathway in IGF‑1 protects endothelial progenitor cells against injury from oxidized LDLs". Molecular Medicine Reports 19.1 (2019): 660-666.
Chicago
Jing‑Wen, H., Liu, G., Xiao, L., Wu, Y."Involvement of endothelial nitric oxide synthase pathway in IGF‑1 protects endothelial progenitor cells against injury from oxidized LDLs". Molecular Medicine Reports 19, no. 1 (2019): 660-666. https://doi.org/10.3892/mmr.2018.9633