Open Access

Optimized protocols for γδ T cell expansion and lentiviral transduction

  • Authors:
    • Rui‑Ning Wang
    • Qian Wen
    • Wen‑Ting He
    • Jia‑Hui Yang
    • Chao‑Ying Zhou
    • Wen‑Jing Xiong
    • Li Ma
  • View Affiliations

  • Published online on: January 8, 2019     https://doi.org/10.3892/mmr.2019.9831
  • Pages: 1471-1480
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

γδ T cells are a subset of unconventional T cells that serve a critical role in infectious diseases and various types of cancer. Cell therapy with genetically‑modified γδ T cells is regarded as a promising tool for tumor treatment. However, since γδ T cells constitute a minority of T cells, their large‑scale expansion is difficult to realize in an efficient and cost‑effective manner. In the present study, based on previous studies, culture protocols for γδ T cells were tested using different combinations of isopentenyl pyrophosphate and interleukin 2 in order to satisfy different experimental purposes. One protocol was demonstrated to be the most suitable for lentiviral transduction. These results greatly reinforce the promising prospects of using γδ T cells in basic research and for clinical applications.
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March-2019
Volume 19 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Wang RN, Wen Q, He WT, Yang JH, Zhou CY, Xiong WJ and Ma L: Optimized protocols for γδ T cell expansion and lentiviral transduction. Mol Med Rep 19: 1471-1480, 2019
APA
Wang, R., Wen, Q., He, W., Yang, J., Zhou, C., Xiong, W., & Ma, L. (2019). Optimized protocols for γδ T cell expansion and lentiviral transduction. Molecular Medicine Reports, 19, 1471-1480. https://doi.org/10.3892/mmr.2019.9831
MLA
Wang, R., Wen, Q., He, W., Yang, J., Zhou, C., Xiong, W., Ma, L."Optimized protocols for γδ T cell expansion and lentiviral transduction". Molecular Medicine Reports 19.3 (2019): 1471-1480.
Chicago
Wang, R., Wen, Q., He, W., Yang, J., Zhou, C., Xiong, W., Ma, L."Optimized protocols for γδ T cell expansion and lentiviral transduction". Molecular Medicine Reports 19, no. 3 (2019): 1471-1480. https://doi.org/10.3892/mmr.2019.9831