Open Access

miR‑675 promotes colorectal cancer cell growth dependent on tumor suppressor DMTF1

  • Authors:
    • Xueliang Yang
    • Yang Lou
    • Minghua Wang
    • Chunlei Liu
    • Yanbo Liu
    • Weili Huang
  • View Affiliations

  • Published online on: December 20, 2018     https://doi.org/10.3892/mmr.2018.9780
  • Pages: 1481-1490
  • Copyright : © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

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Abstract

Colorectal cancer (CRC) has become a worldwide health concern, particularly in developing countries. Therefore, the present study focuses on the investigation of oncogenic microRNA (miR)‑675‑3p, and its role in colorectal carcinogenesis. miR‑675‑3p expression was either overexpressed or inhibited in SW480 CRC cells in order to demonstrate its positive effect on the cell proliferation, as determined by MTS and flow cytometry. Then the present study utilized a luciferase assay to demonstrate that cyclin D binding myb like transcription factor 1 (DMTF1) was modulated by miR‑675‑3p directly at its 3'untranslated region. Overexpression or inhibition of miR‑675‑3p affected the expression of DMTF1, as determined by reverse transcription‑quantitative polymerase chain reaction and western blotting. In addition, the overexpression of miR‑675‑3p promoted cell proliferation, whereas the additional introduction of DMTF1 rescued the overgrowth of the SW480 cells. These results were also confirmed in HT29 CRC cells. In summary, the results of the study demonstrated that miR‑675‑3p directly regulated the expression of DMTF1, which contributed to the further regulation of CRC cell proliferation.
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March-2019
Volume 19 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Yang X, Lou Y, Wang M, Liu C, Liu Y and Huang W: miR‑675 promotes colorectal cancer cell growth dependent on tumor suppressor DMTF1. Mol Med Rep 19: 1481-1490, 2019
APA
Yang, X., Lou, Y., Wang, M., Liu, C., Liu, Y., & Huang, W. (2019). miR‑675 promotes colorectal cancer cell growth dependent on tumor suppressor DMTF1. Molecular Medicine Reports, 19, 1481-1490. https://doi.org/10.3892/mmr.2018.9780
MLA
Yang, X., Lou, Y., Wang, M., Liu, C., Liu, Y., Huang, W."miR‑675 promotes colorectal cancer cell growth dependent on tumor suppressor DMTF1". Molecular Medicine Reports 19.3 (2019): 1481-1490.
Chicago
Yang, X., Lou, Y., Wang, M., Liu, C., Liu, Y., Huang, W."miR‑675 promotes colorectal cancer cell growth dependent on tumor suppressor DMTF1". Molecular Medicine Reports 19, no. 3 (2019): 1481-1490. https://doi.org/10.3892/mmr.2018.9780