Open Access

Epithelial‑mesenchymal transition induced by bone morphogenetic protein 9 hinders cisplatin efficacy in ovarian cancer cells

  • Authors:
    • Ying Wang
    • Bin Yang
    • Jinping Zhao
    • Xiaohui Yu
    • Xing Liu
    • Long Zhang
    • Yunjing Zhang
    • Xiaoli Li
    • Zhenhua Zhai
  • View Affiliations

  • Published online on: January 3, 2019     https://doi.org/10.3892/mmr.2019.9814
  • Pages: 1501-1508
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Bone morphogenetic protein 9 (BMP9) belongs to the transforming growth factor‑β (TGF‑β) superfamily, and has been reported to promote cancer cell proliferation and epithelial‑mesenchymal transition (EMT). Cisplatin (DDP) is the first line treatment for ovarian cancer. However, initiation of EMT confers insensitivity to chemotherapy. The present study aimed to verify and examine the mechanisms underlying the effects of BMP9 on treatment with DDP for ovarian cancer. Prior to treatment with DDP, ovarian cancer cells were exposed to BMP9 for 3 days. Following this, cell viability, apoptosis rate and the extent of DNA damage were evaluated to compare the effects of DDP on BMP9‑pretreated and non‑pretreated ovarian cancer cells. In addition, EMT marker expression was evaluated by western blotting and immunofluorescence. The results demonstrated that BMP9 pretreatment inhibited the cytotoxicity of DDP on ovarian cancer cells. Additionally, BMP9‑pretreated ovarian cancer cells had downregulated expression of the epithelial marker E‑cadherin, which was accompanied by an upregulation of the mesenchymal markers N‑cadherin, Snail, Slug, and Twist. Taken together, the findings of the present study indicated that BMP9 conferred resistance to DDP in ovarian cancer cells by inducing EMT. The present study provided valuable insight into the mechanisms of chemotherapy in ovarian cancer and highlighted the potential of BMP9 as a novel therapeutic target for improving cisplatin chemosensitivity.
View Figures
View References

Related Articles

Journal Cover

March-2019
Volume 19 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Yang B, Zhao J, Yu X, Liu X, Zhang L, Zhang Y, Li X and Zhai Z: Epithelial‑mesenchymal transition induced by bone morphogenetic protein 9 hinders cisplatin efficacy in ovarian cancer cells. Mol Med Rep 19: 1501-1508, 2019
APA
Wang, Y., Yang, B., Zhao, J., Yu, X., Liu, X., Zhang, L. ... Zhai, Z. (2019). Epithelial‑mesenchymal transition induced by bone morphogenetic protein 9 hinders cisplatin efficacy in ovarian cancer cells. Molecular Medicine Reports, 19, 1501-1508. https://doi.org/10.3892/mmr.2019.9814
MLA
Wang, Y., Yang, B., Zhao, J., Yu, X., Liu, X., Zhang, L., Zhang, Y., Li, X., Zhai, Z."Epithelial‑mesenchymal transition induced by bone morphogenetic protein 9 hinders cisplatin efficacy in ovarian cancer cells". Molecular Medicine Reports 19.3 (2019): 1501-1508.
Chicago
Wang, Y., Yang, B., Zhao, J., Yu, X., Liu, X., Zhang, L., Zhang, Y., Li, X., Zhai, Z."Epithelial‑mesenchymal transition induced by bone morphogenetic protein 9 hinders cisplatin efficacy in ovarian cancer cells". Molecular Medicine Reports 19, no. 3 (2019): 1501-1508. https://doi.org/10.3892/mmr.2019.9814