LncRNA BANCR participates in polycystic ovary syndrome by promoting cell apoptosis

Yang,Ruirui; Chen,Jianmei; Wang,Li; Deng,Aijing

doi:10.3892/mmr.2018.9793

LncRNA BANCR participates in polycystic ovary syndrome by promoting cell apoptosis

Authors:
- Ruirui Yang
- Jianmei Chen
- Li Wang
- Aijing Deng
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Affiliations: Department of Obstetrics and Gynecology, Binzhou People's Hospital of Shandong, Binzhou, Shandong 256600, P.R. China, Department of Gynaecology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China
Published online on: December 24, 2018 https://doi.org/10.3892/mmr.2018.9793
Pages: 1581-1586
Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].

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Abstract

The long non‑coding RNA (lncRNA) BANCR is a well‑studied lncRNA that serves pivotal roles in various malignancies; however, to the best of our knowledge, its involvement in polycystic ovary syndrome (PCOS) remains unknown. In the present study, the expression levels of lncRNA BANCR were detected in granulosa cells (GCs) from patients with PCOS and non‑PCOS patients undergoing in vitro fertilization by reverse transcription‑quantitative polymerase chain reaction. Subsequently, GCs and the KGN human granulosa‑like tumor cell line were treated with insulin, and BANCR expression was detected. KGN cells were also transfected with a BANCR expression vector, after which, cell proliferation, apoptosis and the expression levels of pro‑apoptotic B‑cell lymphoma 2‑associated X protein (Bax) and p53 were detected by Cell Counting kit‑8 assay, MTT assay and western blotting, respectively. The results revealed that the expression levels of lncRNA BANCR in GCs were significantly higher in patients with PCOS compared with in non‑PCOS patients. In addition, insulin treatment significantly upregulated the expression of BANCR in GCs and KGN cells. Transfection with the BANCR expression vector significantly inhibited proliferation and promoted apoptosis of KGN cells, and significantly promoted the expression levels of pro‑apoptotic Bax and p53. Therefore, it may be concluded that lncRNA BANCR participates in PCOS by promoting cell apoptosis through the upregulation of Bax and p53.

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1	Azziz R, Carmina E, Chen ZJ, Dunaif A, Laven JS, Legro RS, Lizneva D, Natterson-Horowtiz B, Teede HJ and Yildiz BO: Polycystic ovary syndrome. Nat Rev Dis Primers. 2:160572016. View Article : Google Scholar : PubMed/NCBI
2	Franks S: Polycystic ovary syndrome. Medicine. 45:527–531. 2017. View Article : Google Scholar
3	Ollila MM, West S, Keinänen-Kiukaanniemi S, Jokelainen J, Auvinen J, Puukka K, Ruokonen A, Järvelin MR, Tapanainen JS, Franks S, et al: Overweight and obese but not normal weight women with PCOS are at increased risk of Type 2 diabetes mellitus-a prospective, population-based cohort study. Hum Reprod. 32:423–431. 2017. View Article : Google Scholar : PubMed/NCBI
4	Diamanti-Kandarakis E and Dunaif A: Insulin resistance and the polycystic ovary syndrome revisited: An update on mechanisms and implications. Endocr Rev. 33:981–1030. 2012. View Article : Google Scholar : PubMed/NCBI
5	Franks S, Stark J and Hardy K: Follicle dynamics and anovulation in polycystic ovary syndrome. Hum Reprod Update. 14:367–378. 2008. View Article : Google Scholar : PubMed/NCBI
6	De Leo V, Musacchio MC, Cappelli V, Massaro MG, Morgante G and Petraglia F: Genetic, hormonal and metabolic aspects of PCOS: An update. Reprod Biol Endocrinol. 14:382016. View Article : Google Scholar : PubMed/NCBI
7	Mattick JS and Makunin IV: Non-coding RNA. Hum Mol Genet 15 Spec No. 1:R17–R29. 2006. View Article : Google Scholar
8	Wapinski O and Chang HY: Long noncoding RNAs and human disease. Trends Cell Biol. 21:354–361. 2011. View Article : Google Scholar : PubMed/NCBI
9	Huang X, Hao C, Bao H, Wang M and Dai H: Aberrant expression of long noncoding RNAs in cumulus cells isolated from PCOS patients. J Assist Reprod Genet. 33:111–121. 2016. View Article : Google Scholar : PubMed/NCBI
10	Wang D, Wang D, Wang N, Long Z and Ren X: Long non-coding RNA BANCR promotes endometrial cancer cell proliferation and invasion by regulating MMP2 and MMP1 via ERK/MAPK signaling pathway. Cell Physiol Biochem. 40:644–656. 2016. View Article : Google Scholar : PubMed/NCBI
11	Li R, Zhang L, Jia L, Duan Y, Li Y, Bao L and Sha N: Long non-coding RNA BANCR promotes proliferation in malignant melanoma by regulating MAPK pathway activation. PLoS One. 9:e1008932014. View Article : Google Scholar : PubMed/NCBI
12	Nishi Y, Yanase T, Mu YM, Oba K, Ichino I, Saito M, Nomura M, Mukasa C, Okabe T, Goto K, et al: Establishment and characterization of a steroidogenic human granulosa-like tumor cell line, KGN, that expresses functional follicle-stimulating hormone receptor. Endocrinology. 142:437–445. 2001. View Article : Google Scholar : PubMed/NCBI
13	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
14	Liu Z, Hao C, Huang X, Zhang N, Bao H and Qu Q: Peripheral blood leukocyte expression level of lncRNA steroid receptor RNA activator SRA. and its association with polycystic ovary syndrome: A case control study. Gynecol Endocrinol. 31:363–368. 2015. View Article : Google Scholar : PubMed/NCBI
15	Eppig JJ: Oocyte control of ovarian follicular development and function in mammals. Reproduction. 122:829–838. 2001. View Article : Google Scholar : PubMed/NCBI
16	Legro RS, Finegood D and Dunaif A: A fasting glucose to insulin ratio is a useful measure of insulin sensitivity in women with polycystic ovary syndrome. J Clin Endocrinol Metab. 83:2694–2698. 1998. View Article : Google Scholar : PubMed/NCBI
17	Wang L, Li H, Yang S, Ma W, Liu M, Guo S, Zhan J, Zhang H, Tsang SY, Zhang Z, et al: Cyanidin-3-o-glucoside directly binds to ERα36 and inhibits EGFR-positive triple-negative breast cancer. Oncotarget. 7:68864–68882. 2016.PubMed/NCBI
18	Ni XR, Sun ZJ, Hu GH and Wang RH: High concentration of insulin promotes apoptosis of primary cultured rat ovarian granulosa cells via its increase in extracellular HMGB1. Reprod Sci. 22:271–277. 2015. View Article : Google Scholar : PubMed/NCBI
19	Das M, Djahanbakhch O, Hacihanefioglu B, Saridogan E, Ikram M, Ghali L, Raveendran M and Storey A: Granulosa cell survival and proliferation are altered in polycystic ovary syndrome. J Clin Endocrinol Metab. 93:881–887. 2008. View Article : Google Scholar : PubMed/NCBI
20	Onalan G, Selam B, Baran Y, Cincik M, Onalan R, Gündüz U, Ural AU and Pabuccu R: Serum and follicular fluid levels of soluble Fas, soluble Fas ligand and apoptosis of luteinized granulosa cells in PCOS patients undergoing IVF. Hum Reprod. 20:2391–2395. 2005. View Article : Google Scholar : PubMed/NCBI
21	Gillissen B, Richter A, Richter A, Preissner R, Schulze-Osthoff K, Essmann F and Daniel PT: Bax/Bak-independent mitochondrial depolarization and reactive oxygen species induction by sorafenib overcome resistance to apoptosis in renal cell carcinoma. J Biol Chem. 292:6478–6492. 2017. View Article : Google Scholar : PubMed/NCBI
22	Mei S, Li L, Wei Q, Hao J, Su Y, Mei C and Dong Z: Double knockout of Bax and Bak from kidney proximal tubules reduces unilateral urethral obstruction associated apoptosis and renal interstitial fibrosis. Sci Rep. 7:448922017. View Article : Google Scholar : PubMed/NCBI
23	Wu XQ, Wang YQ, Xu SM, Liu JF, Bi XY, Wang ZQ and Zhang JP: The WNT/β-catenin signaling pathway may be involved in granulosa cell apoptosis from patients with PCOS in North China. J Gynecol Obstet Hum Reprod. 46:93–99. 2017. View Article : Google Scholar : PubMed/NCBI