Anti‑inflammatory effects of Dendropanax morbifera in lipopolysaccharide‑stimulated RAW264.7 macrophages and in an animal model of atopic dermatitis

Choo,Gang‑Sik; Lim,Dong‑Pyo; Kim,Sae‑Man; Yoo,Eun‑Seon; Kim,Sung‑Hyun; Kim,Chang‑Hyun; Woo,Joong‑Seok; Kim,Hyeong‑Jin; Jung,Ji‑Youn

doi:10.3892/mmr.2019.9887

Anti‑inflammatory effects of Dendropanax morbifera in lipopolysaccharide‑stimulated RAW264.7 macrophages and in an animal model of atopic dermatitis

Authors:
- Gang‑Sik Choo
- Dong‑Pyo Lim
- Sae‑Man Kim
- Eun‑Seon Yoo
- Sung‑Hyun Kim
- Chang‑Hyun Kim
- Joong‑Seok Woo
- Hyeong‑Jin Kim
- Ji‑Youn Jung
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Affiliations: Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Chungcheongnam 32439, Republic of Korea, MBG Group, Daejeon 35240, Republic of Korea
Published online on: January 22, 2019 https://doi.org/10.3892/mmr.2019.9887
Pages: 2087-2096
Copyright: © Choo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Dendropanax morbifera (D. morbifera), known as Dendro, means ‘omnipotent drug’ (Panax), and has been called the panacea tree. Various studies on D. morbifera are currently ongoing, aiming to determine its medicinal uses. The present study investigated the anti‑inflammatory effects and underlying mechanism of a natural extract of D. morbifera leaves (DPL) in lipopolysaccharide (LPS)‑stimulated RAW264.7 macrophages. In the present study, the following assays and models were used: MTT assay, nitric oxide (NO) assay, western blotting, ELISA and mouse models of atopic dermatitis. DPL extract markedly reduced the production of NO, inducible NO synthase and interleukin‑6, as well as the nuclear translocation of nuclear factor‑κB (NF‑κB). Additionally, the LPS‑induced activation of extracellular signal‑regulated kinase 1/2 (ERK1/2), P38 and c‑Jun N‑terminal kinase (JNK) was suppressed by DPL extract. Taken together, these results indicate that NF‑κB, ERK1/2, P38 and JNK may be potential molecular targets of DPL extract in the LPS‑induced inflammatory response. Subsequently, the present study investigated the effects of DPL extract in a 2,4‑dinitrochlorobenzene‑induced atopic dermatitis mouse model. Ear thickness, serum immunoglobulin E levels and histological analysis revealed that the DPL extract was effective in attenuating the inflammatory response. These results indicate that DPL extract has anti‑inflammatory potential and may be developed as a botanical drug to treat atopic dermatitis.

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