MiR‑30e and miR‑92a are related to atherosclerosis by targeting ABCA1

Wang,Zhisheng; Zhang,Jiayun; Zhang,Songlan; Yan,Shifang; Wang,Zihui; Wang,Chao; Zhang,Xiaojiang

doi:10.3892/mmr.2019.9983

MiR‑30e and miR‑92a are related to atherosclerosis by targeting ABCA1

Authors:
- Zhisheng Wang
- Jiayun Zhang
- Songlan Zhang
- Shifang Yan
- Zihui Wang
- Chao Wang
- Xiaojiang Zhang
View Affiliations

Affiliations: Medical Department of Shandong Medical College, Shandong 250002, P.R. China, Department of Cardiology, Staff Hospital of Hebei GEO University, Hebei 050031, P.R. China, Second Department of Internal Medicine, People's Hospital of Shizhong District, Shandong 250002, P.R. China, Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, P.R. China, Department of Cardiology, People's Hospital of Mancheng District, Hebei 071000, P.R. China, Department of Cardiology, People's Hospital of Zouping County, Shandong 256200, P.R. China
Published online on: February 25, 2019 https://doi.org/10.3892/mmr.2019.9983
Pages: 3298-3304

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Atherosclerosis is a chronic disease characterized by the accumulation of lipids and fibrous elements in the large arteries, which is the principal cause of coronary artery disease. Dysregulated exosomal microRNA (miRNA) levels in serum have been identified in patients with various diseases, including CAD. In the present study, nine candidate miRNAs were detected in the plasma exosome from 42 patients with coronary atherosclerosis, and a higher expression of miR‑30e and miR‑92a was identified in patients. Following bioinformatics analysis and confirmation through immunoblotting, it was demonstrated that ATP binding cassette (ABC)A1 is a direct target of miR‑30e, and miR‑92a. Furthermore, a negative correlation was identified between plasma miR‑30e and ABCA1, or miR‑30e and cholesterol. Thus, the results of the present study suggest that the miR‑30e level in exosomes from serum may have the potential to be a novel diagnostic biomarker for coronary atherosclerosis.

View Figures

View References

1	Libby P: Inflammation in atherosclerosis. Nature. 420:868–874. 2002. View Article : Google Scholar : PubMed/NCBI
2	Miller GJ and Miller NE: Plasma-high-density-lipoprotein concentration and development of ischaemic heart-disease. Lancet. 1:16–19. 1975. View Article : Google Scholar : PubMed/NCBI
3	Hausenloy DJ and Yellon DM: Targeting residual cardiovascular risk: Raising high-density lipoprotein cholesterol levels. Heart. 94:706–714. 2008. View Article : Google Scholar : PubMed/NCBI
4	Duong PT, Collins HL, Nickel M, Lund-Katz S, Rothblat GH and Phillips MC: Characterization of nascent HDL particles and microparticles formed by ABCA1-mediated efflux of cellular lipids to apoA-I. J Lipid Res. 47:832–843. 2006. View Article : Google Scholar : PubMed/NCBI
5	Abd El-Aziz TA, Mohamed RH and Hagrass HA: Increased risk of premature coronary artery disease in egyptians with ABCA1 (R219K), CETP (TaqIB), and LCAT (4886C/T) genes polymorphism. J Clin Lipidol. 8:381–389. 2014. View Article : Google Scholar : PubMed/NCBI
6	Koopal C, Visseren FL, Kastelein JJ and Westerink J: Premature atherosclerosis, extremely low HDL-cholesterol and concurrent defects in APOA1 and ABCA1 genes: A family case report. Int J Cardiol. 177:e19–e21. 2014. View Article : Google Scholar : PubMed/NCBI
7	Lv YC, Tang YY, Peng J, Zhao GJ, Yang J, Yao F, Ouyang XP, He PP, Xie W, Tan YL, et al: MicroRNA-19b promotes macrophage cholesterol accumulation and aortic atherosclerosis by targeting ATP-binding cassette transporter A1. Atherosclerosis. 236:215–226. 2014. View Article : Google Scholar : PubMed/NCBI
8	Xu Y, Liu Q, Xu Y, Liu C, Wang X, He X, Zhu N, Liu J, Wu Y, Li Y, et al: Rutaecarpine suppresses atherosclerosis in ApoE-/- mice through upregulating ABCA1 and SR-BI within RCT. J Lipid Res. 55:1634–1647. 2014. View Article : Google Scholar : PubMed/NCBI
9	Maegdefessel L: The emerging role of microRNAs in cardiovascular disease. J Intern Med. 276:633–644. 2014. View Article : Google Scholar : PubMed/NCBI
10	Fichtlscherer S, De Rosa S, Fox H, Schwietz T, Fischer A, Liebetrau C, Weber M, Hamm CW, Röxe T, Müller-Ardogan M, et al: Circulating microRNAs in patients with coronary artery disease. Circ Res. 107:677–684. 2010. View Article : Google Scholar : PubMed/NCBI
11	Chistiakov DA, Orekhov AN and Bobryshev YV: Cardiac extracellular vesicles in normal and infarcted heart. Int J Mol Sci. 17(pii): E632016. View Article : Google Scholar : PubMed/NCBI
12	Huber HJ and Holvoet P: Exosomes: Emerging roles in communication between blood cells and vascular tissues during atherosclerosis. Curr Opin Lipidol. 26:412–419. 2015. View Article : Google Scholar : PubMed/NCBI
13	Sayed AS, Xia K, Li F, Deng X, Salma U, Li T, Deng H, Yang D, Haoyang Z, Yang T and Peng J: The diagnostic value of circulating microRNAs for middle-aged (40-60-year-old) coronary artery disease patients. Clinics (Sao Paulo). 70:257–263. 2015. View Article : Google Scholar : PubMed/NCBI
14	Niculescu LS, Simionescu N, Sanda GM, Carnuta MG, Stancu CS, Popescu AC, Popescu MR, Vlad A, Dimulescu DR, Simionescu M and Sima AV: MiR-486 and miR-92a identified in circulating HDL discriminate between stable and vulnerable coronary artery disease patients. PLoS One. 10:e01409582015. View Article : Google Scholar : PubMed/NCBI
15	Zhang T, Tian F, Wang J, Jing J, Zhou SS and Chen YD: Endothelial cell autophagy in atherosclerosis is regulated by miR-30-mediated translational control of ATG6. Cell Physiol Biochem. 37:1369–1378. 2015. View Article : Google Scholar : PubMed/NCBI
16	Suzuki E, Fujita D, Takahashi M, Oba S and Nishimatsu H: Stem cell-derived exosomes as a therapeutic tool for cardiovascular disease. World J Stem Cells. 8:297–305. 2016. View Article : Google Scholar : PubMed/NCBI
17	Raitoharju E, Oksala N and Lehtimäki T: MicroRNAs in the atherosclerotic plaque. Clin Chem. 59:1708–1721. 2013. View Article : Google Scholar : PubMed/NCBI
18	Narahari A, Hussain M and Sreeram V: MicroRNAs as biomarkers for psychiatric conditions: A review of current research. Innov Clin Neurosci. 14:53–55. 2017.PubMed/NCBI
19	Schumacher T and Benndorf RA: ABC transport proteins in cardiovascular disease-A brief summary. Molecules. 22(pii): E5892017. View Article : Google Scholar : PubMed/NCBI
20	Rotllan N, Price N, Pati P, Goedeke L and Fernández-Hernando C: microRNAs in lipoprotein metabolism and cardiometabolic disorders. Atherosclerosis. 246:352–360. 2016. View Article : Google Scholar : PubMed/NCBI
21	Yang Z, Cappello T and Wang L: Emerging role of microRNAs in lipid metabolism. Acta Pharm Sin B. 5:145–150. 2015. View Article : Google Scholar : PubMed/NCBI