Open Access

Identification and functional analysis of microRNAs in rats following focal cerebral ischemia injury

  • Authors:
    • Xianchun Duan
    • Jianghua Gan
    • Dai‑Yin Peng
    • Qiuyu Bao
    • Ling Xiao
    • Liangbing Wei
    • Jian Wu
  • View Affiliations

  • Published online on: March 21, 2019     https://doi.org/10.3892/mmr.2019.10073
  • Pages: 4175-4184
  • Copyright: © Duan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNA sequencing (miRNA‑seq) was performed in the present study to investigate miRNA expression profiles in infarcted brain areas following focal cerebral ischemia induced by middle cerebral artery occlusion in rats. In total, 20 miRNAs were identified to be upregulated and 17 to be downregulated in the infarct area. The expression levels of six differentially expressed miRNAs (DEmiRs), miR‑211‑5p, miR‑183‑5p, miR‑10b‑3p, miR‑182, miR‑217‑5p and miR‑96‑5p, were examined by reverse transcription‑​quantitative polymerase chain reaction. Subsequently, a miRNA‑mRNA network was constructed. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to investigate the functions of the mRNAs targeted by these DEmiRs. The present study aimed to investigate the association between miRNAs and cerebral ischemia to provide potential insight into the molecular mechanisms underlying ischemic stroke.
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May-2019
Volume 19 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Duan X, Gan J, Peng DY, Bao Q, Xiao L, Wei L and Wu J: Identification and functional analysis of microRNAs in rats following focal cerebral ischemia injury. Mol Med Rep 19: 4175-4184, 2019
APA
Duan, X., Gan, J., Peng, D., Bao, Q., Xiao, L., Wei, L., & Wu, J. (2019). Identification and functional analysis of microRNAs in rats following focal cerebral ischemia injury. Molecular Medicine Reports, 19, 4175-4184. https://doi.org/10.3892/mmr.2019.10073
MLA
Duan, X., Gan, J., Peng, D., Bao, Q., Xiao, L., Wei, L., Wu, J."Identification and functional analysis of microRNAs in rats following focal cerebral ischemia injury". Molecular Medicine Reports 19.5 (2019): 4175-4184.
Chicago
Duan, X., Gan, J., Peng, D., Bao, Q., Xiao, L., Wei, L., Wu, J."Identification and functional analysis of microRNAs in rats following focal cerebral ischemia injury". Molecular Medicine Reports 19, no. 5 (2019): 4175-4184. https://doi.org/10.3892/mmr.2019.10073