A novel NHS mutation in a Chinese family with Nance‑Horan Syndrome

Wei,Meirong; Qi,Anhui; Mo,Haiming; Wu,Kailin; Ma,Xu; Wang,Binbin

doi:10.3892/mmr.2019.10106

A novel NHS mutation in a Chinese family with Nance‑Horan Syndrome

Authors:
- Meirong Wei
- Anhui Qi
- Haiming Mo
- Kailin Wu
- Xu Ma
- Binbin Wang
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Affiliations: Department of Ophthalmology, Liuzhou Maternal and Child Healthcare Hospital, Liuzhou, Guangxi 545001, P.R. China, Graduate School of Peking Union Medical College, Beijing 100730, P.R. China, Center for Genetics, National Research Institute for Family Planning, Beijing 100081, P.R. China
Published online on: March 29, 2019 https://doi.org/10.3892/mmr.2019.10106
Pages: 4419-4424

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Abstract

Nance‑Horan syndrome (NHS) is a rare X‑linked disorder with various clinical manifestations. The present study aimed to identify the pathogenic mutation causing NHS in a three‑generation Chinese family with 4 individuals presenting primarily with congenital cataracts. The genomic DNA of 5 individuals was collected, and family history and clinical information were recorded. Whole exome sequencing was performed on the proband, and candidate mutations were filtered by a series of screening processes and validated by Sanger sequencing. The identified pathogenic mutation was confirmed by co‑segregation analysis. Finally, a novel frameshift mutation (NM_001291867.1: c.302dupA; p.Ala102fs) was identified in the NHS actin remodeling regulator (NHS) gene, which co‑segregated with congenital cataracts in this family. Carrier females exhibited similar but milder clinical symptoms compared with the affected male. These clinical symptoms were consistent with the phenotypic features of the NHS‑associated disease, NHS. In summary, the present study identified a novel NHS mutation in a Chinese family with atypical NHS; the results broaden the known pathogenic mutation spectrum of NHS and will aid in the genetic counseling of patients with NHS. The data from the present study also suggest that genetic analysis may be required for the diagnosis of this disease.

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