Long noncoding RNA NEAT1 sponges miR‑125a‑5p to suppress cardiomyocyte apoptosis via BCL2L12

  • Authors:
    • Hong Yan
    • Huasheng Liang
    • Lie Liu
    • Dongli Chen
    • Qianhuan Zhang
  • View Affiliations

  • Published online on: March 27, 2019     https://doi.org/10.3892/mmr.2019.10095
  • Pages: 4468-4474
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Abstract

Increasing evidence has suggested that long non‑coding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) has critical roles in multiple biological processes; however, few studies have reported on its function in heart disease. The present study indicated that NEAT1 expression is markedly downregulated in cardiomyocytes following ischemia/reperfusion injury in vivo and hydrogen peroxide treatment in vitro. Further experiments suggested that ectopic overexpression of NEAT1 suppresses cardiomyocyte apoptosis induced by hydrogen peroxide, as assessed by TUNEL assay and flow cytometry. In addition, using a dual‑luciferase reporter assay, NEAT1 was demonstrated to directly interact with microRNA (miR)‑125a‑5p and overexpression of miR‑125a‑5p efficiently reversed the stimulatory effect of NEAT1 on B‑cell lymphoma‑2‑like 12 (BCL2L12) expression. Furthermore, the results indicated that NEAT1 inhibits cardiomyocyte apoptosis via regulating the expression of BCL2L12, which appeared to be mediated via miR‑125a‑5p. In conclusion, the present study suggested that NEAT1 functions as a miR sponge to inhibit cardiomyocyte apoptosis and may be a novel therapeutic target for cardiomyocyte apoptosis‑associated heart diseases.
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May-2019
Volume 19 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Yan H, Liang H, Liu L, Chen D and Zhang Q: Long noncoding RNA NEAT1 sponges miR‑125a‑5p to suppress cardiomyocyte apoptosis via BCL2L12. Mol Med Rep 19: 4468-4474, 2019
APA
Yan, H., Liang, H., Liu, L., Chen, D., & Zhang, Q. (2019). Long noncoding RNA NEAT1 sponges miR‑125a‑5p to suppress cardiomyocyte apoptosis via BCL2L12. Molecular Medicine Reports, 19, 4468-4474. https://doi.org/10.3892/mmr.2019.10095
MLA
Yan, H., Liang, H., Liu, L., Chen, D., Zhang, Q."Long noncoding RNA NEAT1 sponges miR‑125a‑5p to suppress cardiomyocyte apoptosis via BCL2L12". Molecular Medicine Reports 19.5 (2019): 4468-4474.
Chicago
Yan, H., Liang, H., Liu, L., Chen, D., Zhang, Q."Long noncoding RNA NEAT1 sponges miR‑125a‑5p to suppress cardiomyocyte apoptosis via BCL2L12". Molecular Medicine Reports 19, no. 5 (2019): 4468-4474. https://doi.org/10.3892/mmr.2019.10095