Open Access

IL-1β stimulates IL-8 production, including prostaglandin E2 receptor EP4-triggered pathways, in synoviocyte MH7A cells

  • Authors:
    • Yasuko Shibata
    • Hideo Kasai
    • Masaaki Shimada
    • Tohru Koitabashi
    • Toshiaki Arai
    • Rika Sai
    • Hiroko Terao
    • Masafumi Horikiri
    • Hideyuki Negishi
    • Keiji Miyazawa
    • Yoshimitsu Abiko
  • View Affiliations

  • Published online on: May 1, 2009     https://doi.org/10.3892/mmr_00000108
  • Pages: 359-363
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Abstract

Prostaglandin E2 (PGE2) is an important modulator of cytokine-driven inflammation. Using GeneChip analysis, we found that interleukin (IL)-1β induces the gene expression of PTGER4, which encodes the PGE2 receptor subtype EP4 (PGE2EP4). This subtype is one of four PGE2 receptors occurring in synoviocyte MH7A cells. Immunofluorescence microscopy revealed a corresponding upregulation in the production of PGE2EP4 protein in IL-1β-pretreated MH7A cells. PGE2 alone has no effect on IL-8 production, but in cells pretreated with IL-1β it markedly enhances IL-8 production. Moreover, a stimulatory effect of PGE2 on IL-8 production in the synoviocyte MH7A cells was observed. These results indicate that, in the synovial tissues of patients with rheumatoid arthritis, PGE2 stimulates the release of IL-8 from the fibroblastic cells classified as present, thereby exacerbating inflammation.

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May 2009
Volume 2 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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APA
Shibata, Y., Kasai, H., Shimada, M., Koitabashi, T., Arai, T., Sai, R. ... Abiko, Y. (2009). IL-1β stimulates IL-8 production, including prostaglandin E2 receptor EP4-triggered pathways, in synoviocyte MH7A cells. Molecular Medicine Reports, 2, 359-363. https://doi.org/10.3892/mmr_00000108
MLA
Shibata, Y., Kasai, H., Shimada, M., Koitabashi, T., Arai, T., Sai, R., Terao, H., Horikiri, M., Negishi, H., Miyazawa, K., Abiko, Y."IL-1β stimulates IL-8 production, including prostaglandin E2 receptor EP4-triggered pathways, in synoviocyte MH7A cells". Molecular Medicine Reports 2.3 (2009): 359-363.
Chicago
Shibata, Y., Kasai, H., Shimada, M., Koitabashi, T., Arai, T., Sai, R., Terao, H., Horikiri, M., Negishi, H., Miyazawa, K., Abiko, Y."IL-1β stimulates IL-8 production, including prostaglandin E2 receptor EP4-triggered pathways, in synoviocyte MH7A cells". Molecular Medicine Reports 2, no. 3 (2009): 359-363. https://doi.org/10.3892/mmr_00000108