Antitumor effect of extracts from moutan cortex on DLD-1 human colon cancer cells in vitro

  • Authors:
    • Guoqing Xing
    • Zhenya Zhang
    • Jiqiang Liu
    • Honghai Hu
    • Norio Sugiura
  • View Affiliations

  • Published online on: January 1, 2010     https://doi.org/10.3892/mmr_00000218
  • Pages: 57-61
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Abstract

Moutan cortex, the root bark of Paeonia suffruticosa Andrews (Paeoniaceae), is a traditional Chinese medicine widely used for the treatment of various diseases. In the present study, we examined the antiproliferative effect and apoptosis-inducing activity of paeonol and the crude total glycosides (CTG) extracted from moutan cortex against DLD-1 human colon cancer cells in vitro. Cell viability was measured using the WST-8 assay. Apoptosis was detected by Hoechst 33258 fluorescence staining and flow cytometry. Paeonol and the CTG significantly reduced cell viability in a dose- and time-dependent manner. The induction of apoptosis in DLD-1 cells was characterized by morphological changes and an increased percentage of hypodiploid cells. After treatment for 48 h with paeonol (400 µg/ml) or CTG (200 µg/ml), the ratio of apoptotic cells reached 34.79 and 48.12%, respectively. The findings obtained indicate that paeonol and the CTG extracted from moutan cortex have a significant growth-inhibitory effect on human colon cancer cells. This effect may be related to the induction of apoptosis.

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January 2010
Volume 3 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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APA
Xing, G., Zhang, Z., Liu, J., Hu, H., & Sugiura, N. (2010). Antitumor effect of extracts from moutan cortex on DLD-1 human colon cancer cells in vitro . Molecular Medicine Reports, 3, 57-61. https://doi.org/10.3892/mmr_00000218
MLA
Xing, G., Zhang, Z., Liu, J., Hu, H., Sugiura, N."Antitumor effect of extracts from moutan cortex on DLD-1 human colon cancer cells in vitro ". Molecular Medicine Reports 3.1 (2010): 57-61.
Chicago
Xing, G., Zhang, Z., Liu, J., Hu, H., Sugiura, N."Antitumor effect of extracts from moutan cortex on DLD-1 human colon cancer cells in vitro ". Molecular Medicine Reports 3, no. 1 (2010): 57-61. https://doi.org/10.3892/mmr_00000218