20(S)-Ginsenoside Rg3-induced apoptosis in HT-29 colon cancer cells is associated with AMPK signaling pathway

  • Authors:
    • Hai-Dan Yuan
    • Hai-Yan Quan
    • Ya Zhang
    • Sung Hoon Kim
    • Sung-Hyun Chung
  • View Affiliations

  • Published online on: July 8, 2010     https://doi.org/10.3892/mmr.2010.328
  • Pages: 825-831
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Abstract

20(S)-ginsenoside Rg3 [20(S)-Rg3)], one of the main constituents isolated from Panax ginseng, has been shown to have an anti-cancer effect and to induce apoptosis by interfering with several signaling pathways. However, the molecular mechanisms of AMP-activated protein kinase (AMPK) associated with apoptosis in HT-29 colon cancer cells remain unclear. In the present study, we investigated whether 20(S)-Rg3 exerts an anti-proliferative effect and induces apoptosis by modulating the AMPK signaling pathway in HT-29 cells. 20(S)-Rg3-treated cells displayed several apoptotic features, including DNA fragmentation, proteolytic cleavage of poly (ADP-ribose) polymerase (PARP) and morphological changes. 20(S)-Rg3 down-regulated the expression of anti-apoptotic protein B-cell CLL/lymphoma 2 (Bcl2), up-regulated the expression of pro-apoptotic protein of p53 and Bcl-2-associated X protein (Bax), and caused the release of mitochondrial cytochrome c, PARP, caspase-9 and caspase-3. However, 20(S)-Rg3-induced apoptosis was completely abolished in the presence of compound C (AMPK inhibitor) or small interfering RNA for AMPK (siAMPK). In addition, STO-609 (CaMKKβ inhibitor) attenuated 20(S)-Rg3-induced AMPK activation and apoptosis. These results suggest that 20(S)-Rg3-induced apoptosis in HT-29 cells is mediated via the AMPK signaling pathway, and that 20(S)-Rg3 is capable of inducing apoptosis in colon cancer.

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September 2010
Volume 3 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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APA
Yuan, H., Quan, H., Zhang, Y., Kim, S.H., & Chung, S. (2010). 20(S)-Ginsenoside Rg3-induced apoptosis in HT-29 colon cancer cells is associated with AMPK signaling pathway. Molecular Medicine Reports, 3, 825-831. https://doi.org/10.3892/mmr.2010.328
MLA
Yuan, H., Quan, H., Zhang, Y., Kim, S. H., Chung, S."20(S)-Ginsenoside Rg3-induced apoptosis in HT-29 colon cancer cells is associated with AMPK signaling pathway". Molecular Medicine Reports 3.5 (2010): 825-831.
Chicago
Yuan, H., Quan, H., Zhang, Y., Kim, S. H., Chung, S."20(S)-Ginsenoside Rg3-induced apoptosis in HT-29 colon cancer cells is associated with AMPK signaling pathway". Molecular Medicine Reports 3, no. 5 (2010): 825-831. https://doi.org/10.3892/mmr.2010.328