A novel mono-carbonyl analogue of curcumin induces apoptosis in ovarian carcinoma cells via endoplasmic reticulum stress and reactive oxygen species production

  • Authors:
    • Xie Zhang
    • Han-Qing Zhang
    • Guang-Hui Zhu
    • Yu-Huang Wang
    • Xi-Chong Yu
    • Xin-Bo Zhu
    • Guang Liang
    • Jian Xiao
    • Xiao-Kun Li
  • View Affiliations

  • Published online on: December 9, 2011     https://doi.org/10.3892/mmr.2011.700
  • Pages: 739-744
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Abstract

The aim of the present study was to investigate the apoptosis of human ovarian cancer cell lines, A2780 and CP70, induced by a novel curcumin analogue, B19. The proliferation of cells was detected with methyl thiazolyl tetrazolium (MTT) assay and apoptosis was examined by flow cytometry. Reactive oxygen species (ROS) were assessed by the fluorescent indicator DCF-DA. The protein expression of the endoplasmic reticulum (ER) stress pathways, GRP78, XBP-1, ATF-4 and CHOP, was examined with Western blotting. A growth inhibitory effect was observed after treatment with B19 in a dose-dependent manner and with more potential than curcumin. At 20 µM, B19 induced significant apoptosis in CP70 cells. Furthermore, B19 induced the ER stress response, while curcumin had no effect on ER stress. These results suggest that B19 has more effective antitumor properties than curcumin, and is associated with the activation of ER stress and ROS in ovarian cancer cells.

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March 2012
Volume 5 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhang X, Zhang H, Zhu G, Wang Y, Yu X, Zhu X, Liang G, Xiao J and Li X: A novel mono-carbonyl analogue of curcumin induces apoptosis in ovarian carcinoma cells via endoplasmic reticulum stress and reactive oxygen species production. Mol Med Rep 5: 739-744, 2012
APA
Zhang, X., Zhang, H., Zhu, G., Wang, Y., Yu, X., Zhu, X. ... Li, X. (2012). A novel mono-carbonyl analogue of curcumin induces apoptosis in ovarian carcinoma cells via endoplasmic reticulum stress and reactive oxygen species production. Molecular Medicine Reports, 5, 739-744. https://doi.org/10.3892/mmr.2011.700
MLA
Zhang, X., Zhang, H., Zhu, G., Wang, Y., Yu, X., Zhu, X., Liang, G., Xiao, J., Li, X."A novel mono-carbonyl analogue of curcumin induces apoptosis in ovarian carcinoma cells via endoplasmic reticulum stress and reactive oxygen species production". Molecular Medicine Reports 5.3 (2012): 739-744.
Chicago
Zhang, X., Zhang, H., Zhu, G., Wang, Y., Yu, X., Zhu, X., Liang, G., Xiao, J., Li, X."A novel mono-carbonyl analogue of curcumin induces apoptosis in ovarian carcinoma cells via endoplasmic reticulum stress and reactive oxygen species production". Molecular Medicine Reports 5, no. 3 (2012): 739-744. https://doi.org/10.3892/mmr.2011.700