Effects of IL-1β on the proliferation and apoptosis of gastric epithelial cells and acid secretion from isolated rabbit parietal cells

Guo,Tao; Qian,Jia-Ming; Zhao,Yu-Qing; Li,Xiao-Bo; Zhang,Jian-Zhong

doi:10.3892/mmr.2012.1165

Effects of IL-1β on the proliferation and apoptosis of gastric epithelial cells and acid secretion from isolated rabbit parietal cells

Authors:
- Tao Guo
- Jia-Ming Qian
- Yu-Qing Zhao
- Xiao-Bo Li
- Jian-Zhong Zhang
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Affiliations: Department of Gastroenterology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, P.R. China, Institute of Hepatology, People's Hospital, Beijing University, Beijing 100044, P.R. China , Department of Diagnosis, Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, P.R. China
Published online on: November 2, 2012 https://doi.org/10.3892/mmr.2012.1165
Pages: 299-305

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Abstract

The aim of the present study was to explore the effects of IL-1β on the proliferation and apoptosis of gastric epithelial cells and acid secretion from isolated rabbit parietal cells. The mechanisms by which these effects are mediated were also investigated. Parietal cells were isolated from rabbit gastric mucosa by elutriation. The AGS human gastric cancer cell line, the GES-1 human gastric epithelial cell line and parietal cells were treated with interleukin (IL)-1β in the presence or absence of Helicobacter pylori (H. pylori) for the times indicated. MTT assay and flow cytometry (FCM) were used to determine the levels of proliferation and apoptosis. The expression levels of cyclooxygenase-2 (COX-2) mRNA and protein were examined by RT-PCR and FCM. Acid secretion by parietal cells was examined using 14C-aminopyrine (14C‑AP) accumulation. H+/K+ATPase α subunit mRNA expression was assessed by RT-PCR. The results demonstrated that IL-1β (10 ng/ml) stimulated cellular proliferation and inhibited H. pylori-induced apoptosis in GES-1 and AGS cell lines. IL-1β (10 ng/ml) upregulated the mRNA and protein expression of COX‑2 in GES-1 and AGS cells. Acid secretion stimulated by histamine was identified as significantly inhibited and mRNA expression of H+/K+ATPase α subunit was downregulated by treatment with IL-1β (10 ng/ml) for 30 min and 16 h compared with the control in isolated rabbit parietal cells. The present study demonstrates that IL-1β plays a significant role in H. pylori-induced gastric carcinogenesis through 2 main mechanisms: i) IL-1β may interfere in gastric epithelial cell growth by upregulating COX-2 expression; ii) IL-1β may inhibit the acid secretion from parietal cells by downregulating H+/K+ATPase expression.

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