Effect of Chinese herbal medicine extracts on cell-mediated immunity in a rat model of tuberculosis induced by multiple drug-resistant bacilli

  • Authors:
    • Jun Lu
    • Song Ye
    • Rui Qin
    • Yun Deng
    • Chao-Pin Li
  • View Affiliations

  • Published online on: May 27, 2013     https://doi.org/10.3892/mmr.2013.1491
  • Pages: 227-232
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Abstract

Drug-resistant Mycobacterium tuberculosis poses a major threat to public health. The present study aimed to investigate the effects of Radix Ranunculi Ternati, Radix Sophorae Flavescentis, Prunella Vulgaris L. and Stellera Chamaejasme L. extracts on cell‑mediated immunity in a rat model of tuberculosis (TB) induced by multiple drug-resistant bacilli. The bacterium was isolated from patients infected with pulmonary tuberculosis. The immunological response in humans following infection with Mycobacterium tuberculosis involves a number of cytokines, including IFN-γ and IFN-α, which are important for killing intracellular micro-organisms. T helper type 2 (Th2) cells express numerous cytokines, including IL-4 and IL-10, which mainly participate in humoral immunity and induce the phagocytosis of extracellular bacteria and parasites. In the present study, rats were infected with multiple drug-resistant TB (MDR-TB) in order to establish an MDR-TB model. Peripheral blood mononuclear cells (PBMCs) were isolated and cultivated, and the serum levels of IFN-γ, IL-4, IL-10 and IL-12 were examined by ELISA. The mRNA expression levels of certain cytokines in PBMCs were additionally detected using RT-PCR. The serum levels of IFN-γ in the Radix Ranunculi Ternati, Radix Sophorae Flavescentis, Prunella Vulgaris L. and Stellera Chamaejasme L. groups were 2.01±0.73, 1.92±0.56, 1.98±0.67 and 1.94±0.59 pg/ml; IL-4 levels were 6.01±1.46, 6.12±1.35, 6.47±1.46 and 6.15±1.44 pg/ml; IL-10 levels were 12.09±3.07, 12.45±4.01, 12.13±3.43 and 12.54±3.78 pg/ml; and IL-12 levels were 2.99±0.89, 2.75±0.84, 3.02±0.86 and 2.89±0.75 pg/ml, respectively. These differences were significant compared with the model group (P<0.05). RT-PCR analysis revealed a significant increase in the levels of IFN-γ and IL-12, and a significant decrease in the mRNA levels of IL-4 and IL-10 (P<0.05). These results indicated that the extracts of Radix Ranunculi Ternati, Radix Sophorae Flavescentis, Prunella Vulgaris L. and Stellera Chamaejasme L. are capable of enhancing cell‑mediated immunity in rats by upregulating the levels of genetic transcription. This may explain the observed therapeutic effect of Chinese herbal medicines in the treatment of MDR-TB.

References

1 

Telenti A and Iseman M: Drug-resistant tuberculosis: what do we do now? Drugs. 59:171–179. 2000. View Article : Google Scholar : PubMed/NCBI

2 

Du Toit LC, Pillay V and Danckwerts MP: Tuberculosis chemotherapy: current drug delivery approaches. Respir Res. 7:1182006.PubMed/NCBI

3 

Zhang ZL, Wu XJ, Wang L and Zhang HX: Study on immunocompetence of active constituent of Radix Ranunculi Ternati. Zhong Hua Zhong Yi Yao Xue Hui She. 22:120–122. 2007.(In Chinese).

4 

Fu XR, Li JC and Zhang MZ: Advances in modern research on prunella spike. Zhong Yi Yan Jiu Bian Ji Bu. 18:60–62. 2005.(In Chinese).

5 

Ji RL, Xia SH and Li F: Oxymatrine inhibits MMP-2 expression and reduces cell invasion in human pancreatic carcinoma cell line SW1990. Shi Jie Hua Ren Xiao Hua Za Zhi Bian Ji Bu. 19:19–24. 2011.(In Chinese).

6 

Wu HD and Wang L: Research of antituberculotic. Medical Review. 13:475–478. 2007.

7 

Lu J, Ye S, Deng Y, et al: Effects of four extracts of Chinese herbal medicines on cellular immunity in rats induced by multiple drugs resistant bacillus tuberculosis among pneumoconiosis patients complicated with tuberculosis. Zhong Hua Wei Sheng Wu Xue He Mian Yi Xue Za Zhi Bian Ji Bu. 31:893–897. 2011.(In Chinese).

8 

Bai J, Sun HF and Chen XF: Studies on the anti-mycobacterium tuberculosis activity of 4 Chinese medicinal herbs. Shi Zhen Guo Yi Guo Yao Bian Ji Bu. 18:77–78. 2007.

9 

Liu MX, Sun FJ, Wang CX, et al: Effects of the extract of Coptidis Decoction for detoxification in MDR model rats on cell apoptosis and correlative study. Zhong Yao Cai Bian Ji Bu. 32:1270–1272. 2009.(In Chinese).

10 

Li JG, Gao AL, Liu XH and Li NN: The effect of crewels pills on protection and pathological changes degree of internal organs for mouse suffering MDR-TB. He Nan Zhong Yi Xue Yuan Xue Bao Bian Ji Bu. 23:28–29. 2008.(In Chinese).

11 

Guo XM, Hong YH and Zhou ZL: Advances in studies on chemical constituents from plants of Ranunculus. Zhong Cao Yao Bian Ji Bu. 32:748–750. 2001.(In Chinese).

12 

Wang AW, Wang M, Yuan JR, et al: The study on antitumor effects in vitro of different extracts in Radix Ranunculi Ternati. Tian Ran Chan Wu Yan Jiu Yu Kai Fa Bian Ji Bu. 16:529–531. 2004.(In Chinese).

13 

Liu XL, Zhang Y and Liu XL: Anti-coxsackievirus B3 effects of Sophora flavescens alkaloids in vitro. Shen Yang Yao Ke Da Xue Xue Bao Bian Ji Bu. 23:724–730. 2006.(In Chinese).

14 

Liu D, Jiang YP and Cheng ZL: Sheltering effect on acute and chronic liver injury of mice due to drug combination of glycyrrhizin with matrine to carbon tetrachloride. 30:581–582. 2007.

15 

Feng L, Jia XB, Cheng Y and Li X: Advances in chemical constituents of Prunella vulgaris and antitumor mechanisms. Zhong Hua Zhong Yi Yao Xue Hui She. 23:428–434. 2008.(In Chinese).

16 

Zhang MZ and Wang XQ: Effects of extract from Prunella vulgaris combined with chemotherapeutic agents on proliferation of lymphoma cells. Zhong Liu Bian Ji Bu. 299:961–964. 2009.(In Chinese).

17 

Fang X, Chang RC, Yuen WH, et al: Immune modulatory effects of Prunella vulgaris L. Int J Mol Med. 15:491–495. 2005.PubMed/NCBI

18 

Yang K, Wang YS, Wang LP, et al: Brief review and clinical analysis of pharmacological action of Stellera chamaejasme L. Yi Xue Xin Xi Bian Ji Bu. 23:2496–2498. 2010.(In Chinese).

19 

Wu SZ and Song JF: Mycobacterium tuberculosis pathogenesis and immunity. Zhong Hua Jie He He Hu Xi Za Zhi Bian Ji Bu. 26:101–103. 2003.(In Chinese).

20 

Cole ST, Brosch R, Parkhill J, et al: Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature. 393:537–544. 1998.

21 

Cooper AM, Dalton DK, Stewart TA, et al: Disseminated tuberculosis in interferon-γ gene-disrupted mice. J Exp Med. 178:2243–2247. 1993.

22 

Zhan L, Dai HC, Yang ZP, et al: The study of expression of SHSP and GLS in peripheral blood lymphocytes of drug resistant tuberculosis patients with ternatolide. Zhong Guo Zhong Yao Za Zhi She. 27:677–679. 2002.(In Chinese).

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July 2013
Volume 8 Issue 1

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APA
Lu, J., Ye, S., Qin, R., Deng, Y., & Li, C. (2013). Effect of Chinese herbal medicine extracts on cell-mediated immunity in a rat model of tuberculosis induced by multiple drug-resistant bacilli. Molecular Medicine Reports, 8, 227-232. https://doi.org/10.3892/mmr.2013.1491
MLA
Lu, J., Ye, S., Qin, R., Deng, Y., Li, C."Effect of Chinese herbal medicine extracts on cell-mediated immunity in a rat model of tuberculosis induced by multiple drug-resistant bacilli". Molecular Medicine Reports 8.1 (2013): 227-232.
Chicago
Lu, J., Ye, S., Qin, R., Deng, Y., Li, C."Effect of Chinese herbal medicine extracts on cell-mediated immunity in a rat model of tuberculosis induced by multiple drug-resistant bacilli". Molecular Medicine Reports 8, no. 1 (2013): 227-232. https://doi.org/10.3892/mmr.2013.1491