Gene expression profiling of lens tumors, liver and spleen in α-crystallin/SV40 T antigen transgenic mice treated with Juzen-taiho-to
- Authors:
- Hua-Chuan Zheng
- Akira Noguchi
- Keiji Kikuchi
- Toshihiko Ando
- Takafumi Nakamura
- Yasuo Takano
View Affiliations
Affiliations: Cancer Research Center, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, P.R. China, Kanagawa Cancer Center Research Institute, Yokohama, Kanagawa 241‑0815, Japan, Ajinomoto Co., Inc., Chuo-ku, Tokyo 104‑8315, Japan, Department of Obstetrics and Gynecology, Kawasaki Medical School, Kurasiki, Okayama 701-0192, Japan
- Published online on: December 10, 2013 https://doi.org/10.3892/mmr.2013.1854
-
Pages:
547-552
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Abstract
The autogenic lens tumors induced by the Simian vacuolating virus 40 (SV40) T antigen in α-crystallin/SV40 T antigen transgenic (TG) mice, provide a tool to screen anti‑tumor reagents in vivo and to clarify the underlying mechanisms. Juzen-taiho-to, a Chinese medicine composed of 10 herbs, was frequently used as an alternative medicine for cancer patients by clinicians and occasionally it was demonstrated to have beneficial effects on the prognosis and general condition of cancer patients. However, it was not scientifically verified. In the present study, the anti-tumor effects and underlying mechanisms of Juzen-taiho-to in the TG mice model was examined using cDNA microarray analysis and the results were confirmed by real-time PCR. The TG mice demonstrated a higher cumulative survival rate after treatment with the drug compared with the control group (P<0.05). Gene chip profiles demonstrated that cell functions involving the membrane, glycoprotein, cell membrane, signal and ionic channel for the lens tumor, the cell cycle, DNA replication, homeobox, mitosis and cell division for the spleen and the acetylation, mitochondrion, ribosomal protein, ribonucleoprotein for the liver, were altered by the administration of Juzen‑taiho-to. The important canonical pathways were those of the mitogen-activated protein kinase (MAPK), the cell cycle and the ribosome for the altered genes of the lens tumor, spleen and liver after drug administration, respectively. From real-time PCR, in the eyeball, epidermal growth factor receptor (Egfr), Rasgrf1 and heat shock protein 1B (Hspa1b) mRNAs were found to be significantly lower in treated lenses than in those not exposed to the drug, while Rps25 mRNA demonstrated the opposite association in the liver. It was suggested that Juzen-taiho-to may prolong the survival time of SV40 T antigen TG mice by improving their nutritional condition, inhibiting the MAPK pathway and strengthening the immune system without causing hepatic toxicity.
View References
|
1
|
Reddy VB, Thimmappaya B, Dhar R, et al:
The genome of simian virus 40. Science. 200:494–502. 1978.
View Article : Google Scholar : PubMed/NCBI
|
|
2
|
Ahuja D, Sáenz-Robles MT and Pipas JM:
SV40 large T antigen targets multiple cellular pathways to elicit
cellular transformation. Oncogene. 24:7729–7745. 2005. View Article : Google Scholar : PubMed/NCBI
|
|
3
|
Poulin DL and DeCaprio JA: Is there a role
for SV40 in human cancer? J Clin Oncol. 24:4356–4365. 2006.
View Article : Google Scholar : PubMed/NCBI
|
|
4
|
Kroczynska B, Cutrone R, Bocchetta M, et
al: Crocidolite asbestos and SV40 are cocarcinogens in human
mesothelial cells and in causing mesothelioma in hamsters. Proc
Natl Acad Sci USA. 103:14128–14133. 2006. View Article : Google Scholar : PubMed/NCBI
|
|
5
|
Shah KV: SV40 and human cancer: a review
of recent data. Int J Cancer. 120:215–223. 2007. View Article : Google Scholar : PubMed/NCBI
|
|
6
|
Mutti L, Carbone M, Giordano GG and
Giordano A: Simian virus 40 and human cancer. Monaldi Arch Chest
Dis. 53:198–201. 1998.PubMed/NCBI
|
|
7
|
Matker CM, Rizzo P, Pass HI, et al: The
biological activities of simian virus 40 large-T antigen and its
possible oncogenic effects in humans. Monaldi Arch Chest Dis.
53:193–197. 1998.PubMed/NCBI
|
|
8
|
Zheng HC, Nakamura T, Zheng Y, et al: SV40
T antigen disrupted the cell metabolism and the balance between
proliferation and apoptosis in lens tumors of transgenic mice. J
Cancer Res Clin Oncol. 135:1521–1532. 2009. View Article : Google Scholar : PubMed/NCBI
|
|
9
|
Saiki I: A Kampo medicine
“Juzen-taiho-to”--prevention of malignant progression and
metastasis of tumor cells and the mechanism of action. Biol Pharm
Bull. 23:677–688. 2000.
|
|
10
|
Satoh H, Ishikawa H, Ohtsuka M and
Sekizawa K: Japanese herbal medicine in patients with advanced lung
cancer: prolongation of survival. J Altern Complement Med.
8:107–108. 2002. View Article : Google Scholar : PubMed/NCBI
|
|
11
|
Kim EK and Choi EJ: Pathological roles of
MAPK signaling pathways in human diseases. Biochim Biophys Acta.
1802:396–405. 2010. View Article : Google Scholar : PubMed/NCBI
|
|
12
|
Niwa K, Hashimoto M, Morishita S, et al:
Preventive effects of Juzen-taiho-to on N-methyl-N-nitrosourea and
estradiol-17B-induced endometrial carcinogenesis in mice.
Carcinogenesis. 22:587–591. 2001. View Article : Google Scholar : PubMed/NCBI
|
|
13
|
Onishi Y, Yamaura T, Tauchi K, et al:
Expression of the anti-metastatic effect induced by Juzen-taiho-to
is based on the content of Shimotsu-to constituents. Biol Pharm
Bull. 21:761–765. 1998. View Article : Google Scholar : PubMed/NCBI
|
|
14
|
Satyanarayana A and Kaldis P: Mammalian
cell-cycle regulation: several Cdks, numerous cyclins and diverse
compensatory mechanisms. Oncogene. 28:2925–2939. 2009. View Article : Google Scholar : PubMed/NCBI
|
|
15
|
Csikász-Nagy A, Palmisano A and Zámborszky
J: Molecular network dynamics of cell cycle control: transitions to
start and finish. Methods Mol Biol. 761:277–291. 2011.PubMed/NCBI
|
|
16
|
Tsuchiya M, Kono H, Matsuda M, Fujii H and
Rusyn I: Protective effect of Juzen-taiho-to on
hepatocarcinogenesis is mediated through the inhibition of Kupffer
cell-induced oxidative stress. Int J Cancer. 123:2503–2511. 2008.
View Article : Google Scholar : PubMed/NCBI
|
|
17
|
Kamiyama H, Takano S, Ishikawa E, et al:
Anti-angiogenic and immunomodulatory effect of the herbal medicine
‘Juzen-taiho-to’ on malignant glioma. Biol Pharm Bull. 28:2111–116.
2005.PubMed/NCBI
|
|
18
|
Iijima K, Sun S, Cyong JC and Jyonouchi H:
Juzen-taiho-to, a Japanese herbal medicine, modulates type 1 and
type 2 T cell responses in old BALB/c mice. Am J Chin Med.
27:191–203. 1999. View Article : Google Scholar : PubMed/NCBI
|
|
19
|
Rodnina MV and Wintermeyer W: The ribosome
as a molecular machine: the mechanism of tRNA-mRNA movement in
translocation. Biochem Soc Trans. 39:658–662. 2011. View Article : Google Scholar : PubMed/NCBI
|
|
20
|
Cuadrado A and Nebreda AR: Mechanisms and
functions of p38 MAPK signaling. Biochem J. 429:403–417. 2010.
View Article : Google Scholar : PubMed/NCBI
|
