Chloroform fraction of Scutellaria barbata D. Don promotes apoptosis and suppresses proliferation in human colon cancer cells

  • Authors:
    • Ling Zhang
    • Qiaoyan Cai
    • Jiumao Lin
    • Yi Fang
    • Youzhi Zhan
    • Aling Shen
    • Lihui Wei
    • Lili Wang
    • Jun Peng
  • View Affiliations

  • Published online on: December 16, 2013     https://doi.org/10.3892/mmr.2013.1864
  • Pages: 701-706
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Scutellaria barbata D. Don (SB) has long been used as a major component in numerous Chinese medical formulas to clinically treat various types of cancer. Previously, we reported that the extracts of SB were able to suppress colon cancer growth in vivo and in vitro, possibly by inducing cancer cell apoptosis and inhibiting cell proliferation and tumor angiogenesis. However, the anticancer mechanisms of its bioactive ingredients remain largely unclear. In the present study, using three human colon cancer cell lines SW620, HT-29 and HCT-8, the antitumor effect of different solvent fractions of SB were evaluated and the potential underlying molecular mechanisms were investigated. Using an 3-(4, 5-dimethylthiazol-2-yl)‑2,5-diphenyltetrazolium bromide assay, it was revealed that the chloroform fraction of SB (ECSB) exhibited the most potent inhibitory effect on the growth of all three colon cancer cell lines and SW620 cells exhibited the most sensitive response to ECSB treatment (IC50=65 µg/ml). In addition, by performing fluorescence-activated cell sorting, transmission electron microscopy and colony formation assays, it was observed that ECSB significantly induced apoptosis and inhibited proliferation in SW620 cells in a dose‑dependent manner. Furthermore, ECSB treatment resulted in the upregulation of the pro-apoptotic Bax/Bcl-2 ratio and a decrease in the expression of the pro‑proliferative cyclin D1 and cyclin‑dependent kinase 4. The results from the present study may provide a scientific foundation for the development of novel anticancer agents from the bioactive ingredients in the ECSB.
View Figures
View References

Related Articles

Journal Cover

2014-February
Volume 9 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhang L, Cai Q, Lin J, Fang Y, Zhan Y, Shen A, Wei L, Wang L and Peng J: Chloroform fraction of Scutellaria barbata D. Don promotes apoptosis and suppresses proliferation in human colon cancer cells. Mol Med Rep 9: 701-706, 2014
APA
Zhang, L., Cai, Q., Lin, J., Fang, Y., Zhan, Y., Shen, A. ... Peng, J. (2014). Chloroform fraction of Scutellaria barbata D. Don promotes apoptosis and suppresses proliferation in human colon cancer cells. Molecular Medicine Reports, 9, 701-706. https://doi.org/10.3892/mmr.2013.1864
MLA
Zhang, L., Cai, Q., Lin, J., Fang, Y., Zhan, Y., Shen, A., Wei, L., Wang, L., Peng, J."Chloroform fraction of Scutellaria barbata D. Don promotes apoptosis and suppresses proliferation in human colon cancer cells". Molecular Medicine Reports 9.2 (2014): 701-706.
Chicago
Zhang, L., Cai, Q., Lin, J., Fang, Y., Zhan, Y., Shen, A., Wei, L., Wang, L., Peng, J."Chloroform fraction of Scutellaria barbata D. Don promotes apoptosis and suppresses proliferation in human colon cancer cells". Molecular Medicine Reports 9, no. 2 (2014): 701-706. https://doi.org/10.3892/mmr.2013.1864