Upregulation of H19 promotes invasion and induces epithelial-to-mesenchymal transition in esophageal cancer

Huang,Cheng; Cao,Lihua; Qiu,Limin; Dai,Xiaoli; Ma,Linwei; Zhou,Yingting; Li,Huifen; Gao,Min; Li,Weiyong; Zhang,Qing; Han,Koulan; Lv,Hongzhen

doi:10.3892/ol.2015.3165

Upregulation of H19 promotes invasion and induces epithelial-to-mesenchymal transition in esophageal cancer

Authors:
- Cheng Huang
- Lihua Cao
- Limin Qiu
- Xiaoli Dai
- Linwei Ma
- Yingting Zhou
- Huifen Li
- Min Gao
- Weiyong Li
- Qing Zhang
- Koulan Han
- Hongzhen Lv
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Affiliations: Department of Clinical Medicine, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224005, P.R. China, Department of Dermatology, Yancheng City No. 1 People's Hospital, The Fourth Affiliated Hospital of Nantong Medical College, Yancheng, Jiangsu 224005, P.R. China, Department of Thoracic Surgery, Yancheng City No. 1 People's Hospital, The Fourth Affiliated Hospital of Nantong Medical College, Yancheng, Jiangsu 224005, P.R. China, Department of Pathology, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224005, P.R. China
Published online on: April 29, 2015 https://doi.org/10.3892/ol.2015.3165
Pages: 291-296

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Abstract

Long non-coding RNAs (lncRNAs) have previously been reported to be involved in cancer invasion, proliferation and apoptosis. However, the association between the lncRNA, H19, and esophageal cancer (EC) has remained elusive. In the present study, reverse transcription quantitative‑polymerase chain reaction revealed that the expression of H19 was significantly increased and associated with tumor depth and metastasis in 133 EC samples. Furthermore, MTT and Transwell assays revealed that overexpression of H19 in vitro promoted the proliferation and invasion of EC cell lines, whereas knockdown of H19 inhibited the proliferation and invasion of EC cell lines. In addition, it was identified that an upregulation of H19 induced epithelial‑to‑mesenchymal transition, while the opposite effect was observed following the downregulation of H19. In conclusion, H19 has a significant role in the development of EC and may serve as a potential prognostic marker and therapeutic target for EC.

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