Inflammatory myofibroblastic tumor in the head of the pancreas with anorexia and vomiting in a 69-year‑old man: A case report

Introduction

Inflammatory myofibroblastic tumor (IMT) is an uncommon type of mesenchymal tumor (1). The current World Health Organization (WHO) classification for this rare tumor entity is a fibroblastic sarcoma or myofibroblastoma, which is a distinctive neoplasm of intermediate biological potential that may be malignant or aggressive (2). The worldwide incidence of IMT is 0.04–0.7% (3,4) and clinical data has shown that IMTs have a 25% rate of local recurrence, and up to a 5% rate of distant metastasis (2).

IMT mostly occurs in visceral soft tissues, including the lungs, mesentery, omentum, retroperitoneum, pelvis and abdominal soft tissue (5). IMT mostly affects children and adolescents, while being scarcely observed in old people, and generally does not exhibit any gender preference (5). Pancreatic IMT is rare, however previous studies have shown that this type of occurrence may be more common in women (6,7). A total of 60% of pancreatic IMTs are located in the head of the pancreas, while 40% of cases are located in the body and tail (6,8). Surgery is the primary treatment for pancreatic IMT, and in rare cases this may be complemented by treatment with steroids and/or radiation (7,9). The prognosis of the disease is generally favorable, and regular follow-up is necessary.

In the current study, the case of a 69-year-old man who presented to the Shengjing Hospital of China Medical University (Shenyang, China) with symptoms of anorexia, nausea and vomiting caused by an IMT in the head of the pancreas, is reported. Surgical resection was conducted, and the patient had a regular follow-up 3 years later. Written informed consent was obtained from the patient.

Case report

A 69-year-old man was admitted to the Shengjing Hospital of China Medical University on January 9, 2013 with a 3-month history of anorexia, upper abdominal distension and vomiting. Nausea and vomiting frequently occurred following a meal. Undigested food and bile were occasionally present in the vomitus. Pain, fever, jaundice and melena were not reported, although the patient had experienced a weight loss of 10 kg. His medical history was unremarkable. Physical examination revealed absence of tenderness in the epigastric area. Succussion splash and Murphy's sign were negative, and the patient's bowel sounds were normal. Laboratory tests, including complete blood count, urinalysis, amylase test and lipase test, were normal. The levels of tumor markers, including α-fetoprotein, 2.20 ng/ml (normal range, 0.00–9.00 ng/ml), carcinoembryonic antigen (CEA), 2.87 ng/ml (normal range, 0.00–5.00 ng/ml), carbohydrate antigen (CA)19-9, 15.26 U/ml (normal range, 0.00–37.00 U/ml) and CA72-4, 1.30 U/ml (normal range, 0.00–6.90 U/ml), were negative. Enhanced abdominal computed tomography (CT; SOMATOM Definition AS; Siemens Healthcare, Erlangen, Germany) examination revealed a cystic-solid tumor located between the pancreas and the duodenum (Fig. 1A). No evidence of distant organ or lymph node metastasis was observed. Endoscopy revealed a anabrotic protrusion lesion located between the duodenal bulb and the descendant duodenum (Fig. 1B). Endoscopic ultrasound (PENTAX EG-2970K and PENTAX EG-3870UTK; Pentax, Tokyo, Japan) examination revealed a 23.0×19.0-mm protrusive low-echo mass, which had unclear boundaries with the adjacent pancreas, located between the duodenal bulb and the descendant duodenum (Fig. 1C). The endoscopic biopsy of the mass was inconclusive, since it demonstrated a fibrous lesion containing inflammatory cells without any evidence of malignancy. Since malignant pancreatic head enlargement with duodenum metastasis was suspected, the patient underwent an operation in order to establish a diagnosis by operative pathological biopsy. During the operation, a large, hard mass with a diameter of ~4.0×3.0 cm, which was located in the head of the pancreas and metastasized into the duodenum, was identified. The gross specimen was fleshy, with a white cut surface. Necrosis and hemorrhage were observed (Fig. 2). Histopathologically, the tumor cells were arranged mainly in a fusiform pattern with an inflammatory infiltrate of lymphocytes and eosinophils (Fig. 3). Samples were stained with hematoxylin and eosin (Sigma-Aldrich, St. Louis, MO, USA) and visualized using a microscope (Eclipse E800; Olympus Corporation, Tokyo, Japan). Immunohistochemical staining was performed with the following antibodies: mouse monoclonal anti-human smooth muscle actin (SMA; cat. no. ZM-0003; dilution, 1:50; Beijing Zhongshan Jinqiao Biological Technology Co., Ltd., Beijing, China), mouse monoclonal anti-human desmin (cat. no. ZM-0091; dilution, 1:50; Beijing Zhongshan Jinqiao Biological Technology Co., Ltd.), mouse monoclonal anti-human β-catenin (cat. no. ZM-0442; dilution, 1:50; Beijing Zhongshan Jinqiao Biological Technology Co., Ltd.), mouse monoclonal anti-human cluster of differentiation (CD)34 (cat. no. ZM-0046; dilution, 1:50; Beijing Zhongshan Jinqiao Biological Technology Co., Ltd.), mouse monoclonal anti-human CD117 (cat. no. ZM-0437; dilution, 1:50; Beijing Zhongshan Jinqiao Biological Technology Co., Ltd.) and mouse monoclonal anti-human DOG-1 (cat. no. ZM-0371; dilution, 1:50; Beijing Zhongshan Jinqiao Biological Technology Co., Ltd.). Phosphate-buffered saline (Beijing Zhongshan Jinqiao Biological Technology Co., Ltd.) was used as the dilution buffer and tissue sections were incubated with the primary antibodies overnight at 4°C. The sections were then washed twice and incubated with horseradish peroxidase-conjugated goat anti-mouse IgG secondary antibodies (cat. no. SP-9002; ready to use; Beijing Zhongshan Jinqiao Biological Technology Co., Ltd.) for 60 min at room temperature. Staining was visualized using a microscope (Eclipse E800; Olympus Corporation). Immunohistochemically, the tumor was positive for SMA (Fig. 4A), desmin (Fig. 4B) and β-catenin (Fig. 4C), but negative for CD34 (Fig. 4D), CD117 (Fig. 4E) and DOG-1 antibody (Fig. 4F). A pathological diagnosis of IMT was established based on the histology and immunohistochemistry results. The postoperative course was uneventful, and the patient was discharged from hospital on January 30, 2013. No adjuvant treatment was administered following surgical excision of the tumor, and no evidence of a recurrent tumor was observed during the 3 year follow-up.

Figure 1. Enhanced abdominal CT, endoscopy and endoscopic ultrasound examination of the lesion. (A) Enhanced abdominal CT revealed an irregular low-density mass that extended into the head of the pancreas and duodenum (red circle). Tortuous and dilated lumens were observed in the mass (white arrow). (B) Endoscopy revealed an ulcerated, protruding lesion in the duodenal bulb and the descending duodenum. (C) Endoscopic ultrasound revealed a 23.0×19.0-mm unorganized mass with an ill-defined border that extended into the duodenal bulb and the descending duodenum. CT, computed tomography.

Figure 2. Photograph of the resected specimen revealing the macroscopic appearance of the fleshy, lobulated and circumscribed mass (4.0×3.0 cm) with mixed solid and cystic components, which was located in the head of the pancreas. Necrosis and hemorrhage were observed.

Figure 3. Photomicrograph of hematoxylin and eosin staining of the lesion. (A) A fusiform pattern with a variable amount of inflammatory infiltrate composed of lymphocytes and eosinophils in a myxoid matrix was observed. Magnification, ×100. (B) Nuclear pleomorphism and mitotic figures were not prominent, but occasional atypical cells with large nuclei and necrotic cystic components were observed. Magnification, ×200.

Figure 4. Photomicrograph of immunohistochemistry staining of the lesion. (A) Tumor cells were positive for smooth muscle actin. Smooth muscle of blood vessels acted as an internal positive control. Tumor cells were immunopositive for (B) desmin and (C) β-catenin, but immunonegative for (D) CD34. Endothelial cells of blood vessels were used as an internal positive control. Tumor cells were immunonegative for (E) CD117 and (F) DOG-1 antibody. Magnification, ×200. CD, cluster of differentiation.

Discussion

IMT, which is clinically rare, was considered to be a reactionary inflammatory lesion following trauma, surgery or infection, and was previously referred to as an inflammatory pseudotumor, plasma cell granuloma, xanthomatous pseudotumor, xanthoma or histiocytoma (10,11). Subsequent studies have identified that IMT is a neoplasm with frequent clonal alterations in the chromosome 2p23 and with the potential for local aggressiveness, recurrence, metastasis, malignant transformation and myofibroblastic differentiation (11–13). The current WHO classification for this rare tumor entity is a fibroblastic sarcoma or myofibroblastoma, which is a distinctive neoplasm of intermediate biological potential that may be malignant or aggressive (2,10,14). IMT principally affects young patients, and does not exhibit any gender preference. IMT may occur in the whole body, although the most common sites are the lungs, head, neck, extremities, intra-abdominal and retroperitoneal soft tissues (10,11,15). To the best of our knowledge, a rare case of a pancreatic IMT that caused intestinal obstruction in an old man has been previously published (8). Despite being previously regarded as a reactive lesion, IMT is currently considered to be a neoplastic process (10,11). Therefore, its correct diagnosis is critical for offering the patients the appropriate disease management and subsequent treatment.

The clinical presentation of IMT varies depending on its anatomic location and whether it involves vital organs (16). For patients with IMT, pancreatic involvement is often inconclusive, since patients may generally present with nonspecific symptoms such as abdominal pain, weight loss and jaundice (11). In rare cases, IMT may present with anemia, fatigue, sudden diabetes or a palpable mass that resembles pancreatic carcinoma, periampullary carcinoma, lymphoma and endocrine tumors (11). The radiological features of IMT are nonspecific, and exhibit variable characteristics (6). Ultrasound and CT examinations reveal a solid, or occasionally cystic-solid, mass in the pancreas, which is usually sized between 5.0 and 10.0 cm and may be well-demarcated or metastatic (7).

Due to its nonspecific symptoms and imaging findings, definitive diagnosis of IMT relies on histological evaluations (6). However, it is often difficult to obtain enough tissue using a transendoscopic biopsy to establish a definitive histological diagnosis (17). In the present patient, a gastroscopic biopsy of the lesion was inconclusive, and surgical resection of the tissues aided to obtain the final diagnosis of IMT.

The histology results of the present case revealed the presence of an independent cell type between smooth muscle cells and fibroblasts, in addition to mixed spindle cells scattered among a large number of inflammatory cells, mostly lymphocytes, plasma cells, organized cells, eosinophils and neutrophils (14). It has been previously reported that three histological patterns may coexist variably within the same tumor, namely myxoid/vascular, compact spindle cell and fibrous hypocellular (15,18–20). Immunohistochemically, the majority of cases of IMT express SMA, desmin and cytokeratin (15,17,21). Anaplastic lymphoma kinase (ALK) immunostaining is detected in 40–60% of cases, and involves rearrangements or translocations of the ALK receptor tyrosine kinase on the chromosome 2p23 (16,21). In previous studies, the histological features of IMT did not correlate with its clinical behavior (2). In addition, the risk of malignant transformation or metastasis has been described in certain cases, and remains low (<5%) (2). In the present case, the tumor exhibited a compact spindle cell histological pattern, and there were no obvious signs of malignancy in the histology. Immunohistochemical staining demonstrated the tumor to be SMA+, desmin+, β-catenin+, CD34−, CD117− and DOG-1−. During a regular 3 year follow-up, the patient was observed to be healthy.

Currently, despite the difficulty of obtaining a definitive diagnosis based on biopsies and radiological examinations, surgery with complete excision remains the primary therapeutic option for IMT, although no real consensus regarding the treatment of IMT exists (7,9). The low risk of malignant transformation or metastasis has been described in previous cases of IMT, but if the excision is incomplete, the risk increases from <5 to 25% (2). Due to the possibility of malignancy and relapse, a number of authors have reported that a simple tumor excision is uncertain (8). Wide excision may be appropriate if the patient's physical condition is adequate (7,9). However, whether radical resection for such intermediate, low-grade lesions is appropriate requires further clinical study. In the present report, the case of an elderly, infirm man who had IMT of the head of the pancreas with unclear boundaries with the adjacent duodenum, was presented. During surgery, no evidence of blood vessels, lymph node or other abdominal organs metastasis (except adjacent duodenum) was observed. In consequence, a duodenopancreatectomy was performed. For similar patients, a surgical approach on a case-by-case basis may be considered in the future. For non-excisable lesions, several medical treatments, including chemotherapy, radiation therapy, nonsteroidal anti-inflammatory drugs, corticosteroids and antitumor necrosis factor-binding antibodies, have been previously administered to palliate or shrink these IMTs to a resectable size and configuration (6,7,9). However, the medical treatment is not clearly defined due to a lack of strong statistical evidence, and palliative treatment programs cannot be recommended as a standard regimen (22,w). Based on the recent advances in ALK inhibitors and other ALK target genes, these therapies may be more effective, and thus may be offered to patients with IMT instead (16). Whether surgical resection or drug therapy is the selected treatment, the prognosis of the disease is favorable, with distant metastases occurring rarely, and a 5-year survival rate of 91% (10,11,15). However, close, long-term follow-up is necessary due to the probability of malignancy, distant metastasis and recrudescence exhibited by IMT (15).

In conclusion, in this study the rare case of pancreatic head IMT in a 69-year-old man, with aggressive behavior and metastasis to the duodenum was presented. However, the patient's clinical symptoms and imaging findings were nonspecific and diagnosis relied on histopathological examination and immunohistochemistry. Complete surgical resection is the preferred treatment for IMT. The prognosis of the disease is generally favorable, and regular follow-up is necessary.

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