Novel recombinant protein FlaA N/C increases tumor radiosensitivity via NF‑κB signaling in murine breast cancer cells

Xu,Ying; Wu,Dongming; Fan,Yuanchun; Li,Peigeng; Du,Hongfei; Shi,Jiao; Wang,Dan; Zhou,Xiaoping

doi:10.3892/ol.2016.4957

Novel recombinant protein FlaA N/C increases tumor radiosensitivity via NF‑κB signaling in murine breast cancer cells

Authors:
- Ying Xu
- Dongming Wu
- Yuanchun Fan
- Peigeng Li
- Hongfei Du
- Jiao Shi
- Dan Wang
- Xiaoping Zhou
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Affiliations: Clinical Laboratory, The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan 610041, P.R. China
Published online on: August 5, 2016 https://doi.org/10.3892/ol.2016.4957
Pages: 2632-2640
Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The recombinant protein flagellin A (FlaA) N/C, derived from the flagellin protein of Legionella pneumophila, has been shown to increase the expression of cytoprotective cytokines, activate the nuclear factor‑κB (NF‑κB) signaling pathway, and increase the survival of mice following total body irradiation. Determi ning whether FlaA N/C has a sensitizing effect on tumor radiation or a direct tumoricidal effect is critical for its application as an effective radiation protection agent. The present study investigated the molecular mechanism underlying the tumor radiosensitivity of FlaA N/C. FlaA N/C was found to increase tumor apoptosis and autophagy, regulate the cell cycle and increase radiosensitivity in 4T1 tumor cells. Furthermore, FlaA N/C was found to promote radiosensitivity by activating NF‑κB signaling. Finally, the present study analyzed FlaA N/C‑enhanced radiosensitivity in animal models, and FlaA N/C was found to significantly prolong the survival period of mice after total body radiation. This indicates that FlaA N/C might be a novel radiation sensitizer in tumor radiation therapy.

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