Effects of Hedera helix L. extracts on rat prostate cancer cell proliferation and motility

Gumushan‑Aktas,Hatice; Altun,Seyhan

doi:10.3892/ol.2016.4941

Effects of Hedera helix L. extracts on rat prostate cancer cell proliferation and motility

Authors:
- Hatice Gumushan‑Aktas
- Seyhan Altun
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Affiliations: Department of Biology, Faculty of Arts and Science, Harran University, Osmanbey Campus, 63300 Sanliurfa, Turkey, Department of Biology, Faculty of Science, Istanbul University, 34134 Istanbul, Turkey
Published online on: August 3, 2016 https://doi.org/10.3892/ol.2016.4941
Pages: 2985-2991

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Abstract

Hedera helix L., a member of Araliaceae family, has antiproliferative, cytotoxic, antimicrobial, antifungal, antiprotozoal and anti‑inflammatory effects, and is used in cosmetics. The aim of the present study was to investigate the effect of treatment with extracts of leaves and unripened fruits of H. helix on rat prostate cancer cell lines with markedly different metastatic potentials: Mat‑LyLu cells (strongly metastatic) and AT‑2 cells (weakly metastatic). The effects of the extracts on cell kinetics and migration were determined. Tetrodotoxin was used to block the voltage‑gated sodium channels (VGSCs) associated specifically with Mat‑LyLu cells. Cell proliferation was detected spectrophotometrically using the 3-(4,5-dimethyl‑2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. The mitotic index was determined using the Feulgen staining method. Lateral motility was quantified by wound‑healing assays. The results of the present study demonstrated that cell kinetics (proliferation and mitotic activity) and motility were inhibited by ethanolic leaf extract of H. helix. The ethanolic extract of H. helix fruit suppressed Mat‑LyLu cell migration, with no effect on proliferation. The opposite effects were observed in AT‑2 cells; migration was not affected but proliferation was inhibited. In conclusion, the ethanolic fruit extract of H. helix may inhibit the cell migration of Mat‑LyLu cells by blocking VGSCs. However, the effect of ethanolic leaf extract of H. helix treatment on the lateral motility of the cancer cells is unclear.

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