Osteopontin improves adhesion and migration of human primary renal cortical epithelial cells during wound healing

Wu,Jinfeng; Wang,Zuolin

doi:10.3892/ol.2016.5219

Osteopontin improves adhesion and migration of human primary renal cortical epithelial cells during wound healing

Authors:
- Jinfeng Wu
- Zuolin Wang
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Affiliations: Laboratory of Oral Biomedical Science and Translational Medicine, School of Stomatology, Tongji University, Shanghai 200072, P.R. China
Published online on: October 4, 2016 https://doi.org/10.3892/ol.2016.5219
Pages: 4556-4560
Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the effect of osteopontin (OPN) on adhesion and migration in human primary renal cortical epithelial cells during wound healing and Transwell assays. MTT assay was used to examine the cell viability and western blot analysis was used to examine the expression of cytoskeletal proteins and cell adhesion molecules. The results showed that overexpression of OPN had positive effects on the viability, proliferation, adhesion and migration of the human primary renal cortical epithelial cells. In addition, the integrity of the cell membrane and cytoskeleton of the epithelial cells was negatively affected by knockdown of OPN expression. The Transwell migration and a wound healing assays performed using OPN‑knockdown cells suggested that OPN had a significant impact on cell migration (P=0.0421) and wound healing (P=0.0333). Therefore, OPN may be a potential target for the therapeutic modulation of skin repair to improve the healing rate and quality of wound healing.

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