Open Access

PARP-1 may be involved in angiogenesis in epithelial ovarian cancer

  • Authors:
    • Wei Wei
    • Yan Li
    • Shuqing Lv
    • Cancan Zhang
    • Yongjie Tian
  • View Affiliations

  • Published online on: October 5, 2016     https://doi.org/10.3892/ol.2016.5226
  • Pages: 4561-4567
  • Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Poly (ADP-ribose) polymerase 1 (PARP-1) is involved in DNA repair and has been implicated in chemoresistance. The present study investigated whether PARP‑1 promotes angiogenesis in ovarian cancer. PARP‑1 and vascular endothelial growth factor A (VEGF-A) expression and CD34+ microvascular density (MVD) were assessed using immunohistochemistry in 60 human epithelial ovarian cancer specimens. PARP‑1 was stably knocked‑down in SKOV3 cells using a specific small interfering RNA (siRNA); angiogenic capacity was assessed using the human umbilical vein endothelial cell (HUVEC) tubule formation assay; and PARP‑1 and VEGF‑A expression were examined by reverse transcription‑quantitative polymerase chain reaction, western blotting and ELISA. PARP‑1 was found to be expressed in 73.3% (44/60) of the human epithelial ovarian cancer specimens and was significantly associated with VEGF‑A, MVD, tumor size, histological grade and lymphatic metastasis (P<0.05). Compared with cells transfected with a negative control siRNA, knockdown of PARP‑1 significantly suppressed the ability of SKOV3 cell‑conditioned media to promote HUVEC tubule formation on Matrigel in vitro. Knockdown of PARP‑1 in SKOV3 cells also significantly reduced VEGF‑A mRNA and protein expression and secretion. In summary, PARP‑1 is overexpressed and may enhance angiogenesis in epithelial ovarian cancer by upregulating VEGF-A.
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December-2016
Volume 12 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Wei W, Li Y, Lv S, Zhang C and Tian Y: PARP-1 may be involved in angiogenesis in epithelial ovarian cancer. Oncol Lett 12: 4561-4567, 2016
APA
Wei, W., Li, Y., Lv, S., Zhang, C., & Tian, Y. (2016). PARP-1 may be involved in angiogenesis in epithelial ovarian cancer. Oncology Letters, 12, 4561-4567. https://doi.org/10.3892/ol.2016.5226
MLA
Wei, W., Li, Y., Lv, S., Zhang, C., Tian, Y."PARP-1 may be involved in angiogenesis in epithelial ovarian cancer". Oncology Letters 12.6 (2016): 4561-4567.
Chicago
Wei, W., Li, Y., Lv, S., Zhang, C., Tian, Y."PARP-1 may be involved in angiogenesis in epithelial ovarian cancer". Oncology Letters 12, no. 6 (2016): 4561-4567. https://doi.org/10.3892/ol.2016.5226