Melittin inhibits the invasion of MCF‑7 cells by downregulating CD147 and MMP‑9 expression

Wang,Jianjun; Li,Fengyu; Tan,Jiang; Peng,Xuewei; Sun,Lili; Wang,Ping; Jia,Shengnan; Yu,Qingmiao; Huo,Hongliang; Zhao,Hongyan

doi:10.3892/ol.2016.5516

Melittin inhibits the invasion of MCF‑7 cells by downregulating CD147 and MMP‑9 expression

Authors:
- Jianjun Wang
- Fengyu Li
- Jiang Tan
- Xuewei Peng
- Lili Sun
- Ping Wang
- Shengnan Jia
- Qingmiao Yu
- Hongliang Huo
- Hongyan Zhao
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Affiliations: Laboratory of Molecular and Cellular Physiology, School of Life Science, Northeast Normal University, Changchun, Jilin 130024, P.R. China
Published online on: December 20, 2016 https://doi.org/10.3892/ol.2016.5516
Pages: 599-604
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Tumor invasion and metastasis are the critical steps in determining the aggressive phenotype of human cancers. Melittin, a major component of bee venom, has been reported to induce apoptosis in several cancer cells. However, the mechanisms of melittin involvement in cancer invasion and metastasis remain unclear. Our previous study indicated that melittin inhibits cyclophilin A (CypA), a ubiquitously distributed peptidylprolyl cis‑trans isomerase, in macrophage cells. In the present study, the Transwell assay results showed that melittin may downregulate the invasion level of MCF‑7 cells in a dose‑dependent manner. Additionally, it was also found, using flow cytometry and reverse transcription‑polymerase chain reaction, that melittin decreased the expression of cluster of differentiation (CD)147 and matrix metallopeptidase‑9 (MMP‑9), whereas CypA upregulated the expression of CD147 and MMP‑9. Overall, the present study indicated that melittin decreased the invasion level of MCF‑7 cells by downregulating CD147 and MMP‑9 by inhibiting CypA expression. The results of the present study provide an evidence for melittin in anticancer therapy and mechanisms.

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