Open Access

Antitumor effects and the underlying mechanism of licochalcone A combined with 5-fluorouracil in gastric cancer cells

  • Authors:
    • Xiaolin Lin
    • Lei Tian
    • Lisha Wang
    • Wenyan Li
    • Qi Xu
    • Xiuying Xiao
  • View Affiliations

  • Published online on: January 18, 2017     https://doi.org/10.3892/ol.2017.5614
  • Pages: 1695-1701
  • Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Licochalcone A (LCA) is a flavonoid extracted from licorice root that has antiparasitic, antibacterial and antitumor properties. Previous studies have revealed that LCA may be a novel treatment for gastric cancer. The present study further assessed the potential antitumor effects of LCA alone or in combination with 5‑fluorouracil (5‑FU), and the underlying mechanisms responsible for those effects in gastric cancer cells. The effects of LCA alone or in combination with 5‑FU on SGC7901 and MKN‑45 gastric cancer cell lines were studied using Cell Counting Kit‑8, cell cycle, apoptosis and western blot analyses of cell check points and apoptosis‑associated proteins. The results revealed that LCA inhibited cell proliferation, blocked cell cycle progression at the G2/M transition and induced apoptosis. Western blot analysis demonstrated that LCA treatment increased the levels of tumor proteins 21 and 27, as well as mouse double minute 2 homolog in gastric cancer cells. In addition, LCA treatment increased the expression levels of Bax, cleaved‑poly ADP ribose polymerase, tumor protein 53 and caspase 3, and decreased the expression levels of Bcl‑2. Therefore, the present study demonstrated that LCA alone or in combination with 5‑FU may have significant anticancer effects on gastric cancer cells, and may be a novel therapeutic for the treatment of gastric cancer in the future.
View Figures
View References

Related Articles

Journal Cover

March-2017
Volume 13 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Lin X, Tian L, Wang L, Li W, Xu Q and Xiao X: Antitumor effects and the underlying mechanism of licochalcone A combined with 5-fluorouracil in gastric cancer cells. Oncol Lett 13: 1695-1701, 2017
APA
Lin, X., Tian, L., Wang, L., Li, W., Xu, Q., & Xiao, X. (2017). Antitumor effects and the underlying mechanism of licochalcone A combined with 5-fluorouracil in gastric cancer cells. Oncology Letters, 13, 1695-1701. https://doi.org/10.3892/ol.2017.5614
MLA
Lin, X., Tian, L., Wang, L., Li, W., Xu, Q., Xiao, X."Antitumor effects and the underlying mechanism of licochalcone A combined with 5-fluorouracil in gastric cancer cells". Oncology Letters 13.3 (2017): 1695-1701.
Chicago
Lin, X., Tian, L., Wang, L., Li, W., Xu, Q., Xiao, X."Antitumor effects and the underlying mechanism of licochalcone A combined with 5-fluorouracil in gastric cancer cells". Oncology Letters 13, no. 3 (2017): 1695-1701. https://doi.org/10.3892/ol.2017.5614