Deregulation of miR‑193b affects the growth of colon cancer cells via transforming growth factor‑β and regulation of the SMAD3 pathway
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- Published online on: February 22, 2017 https://doi.org/10.3892/ol.2017.5763
- Pages: 2557-2562
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Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
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Abstract
MicroRNA‑193b (miRNA‑193b) is often differentially expressed and is an important regulator of gene expression in colon cancer. The aim of the present study was to determine whether miRNA‑193b affects cell growth in colon cancer and to investigate the potential underlying mechanisms. Patients with colorectal cancer (CRC; n=20) and healthy volunteers (n=10) were enrolled from the Department of Gastrointestinal Surgery Center, First Affiliated Hospital of Sun Yat‑Sen University (Guangzhou, China). Western blot analysis was used to evaluate the protein expression of SMAD3 and transforming growth factor‑β (TGF‑β) in the patient samples. It was determined that miRNA‑193b expression was markedly elevated in the CRC tissue samples. Furthermore, silencing of miRNA‑193bin SW620 CRC cells by specific inhibitors significantly reduced the cell proliferation and induced apoptosis. In addition, the downregulation of miRNA‑193b significantly activated the protein expression of SMAD3 and TGF‑β, and promoted caspase‑3 activity in SW620 cells. The results of the present study suggested that the deregulation of miRNA‑193b may affect cell growth in colon cancer via the TGF‑β and SMAD3 signaling pathways.
